Avil_00270 : CDS information

Location

Organism	Streptomyces viridochromogenes
Strain	Tü57
Entry name	Avilamycin
Contig	AF333038
Start / Stop / Direction	26,490 / 27,782 / + [in whole cluster] 26,490 / 27,782 / + [in contig]
Location	26490..27782 [in whole cluster] 26490..27782 [in contig]
Type	CDS
Length	1,293 bp (430 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative glycosyltransferase
Product (GenBank)	putative glycosyltransferase
Gene
Gene (GenBank)	aviGT1
EC number
Keyword
Note
Note (GenBank)	AviGT1
Reference	ACC Q93KW3 PmId [11410376] Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics. (Chem Biol. , 2001) comment avilamycin A生合成遺伝子クラスターの報告。 AviGT1: Glycosyltransferase from S. nogalater [Proposed function:Biosynthesis of the heptasaccharide chain] このORFについて機能解析はされていない。 AviGT1は、antibiotic biosynthesisに関与することが知られるglycosyltransferasesに似ている。
Related Reference	ACC Q194P5 NITE Mith_00300 PmId [9733697] Identification of two genes from Streptomyces argillaceus encoding glycosyltransferases involved in transfer of a disaccharide during biosynthesis of the antitumor drug mithramycin. (J Bacteriol. , 1998) comment Streptomyces argillaceus Glycosyltransferase_mtmGI mtmGI,mtmGIIの機能解析 mtmGI,mtmGIIの不活性化株はmithramycinを合成出来なかった。両株の培養上清から検出した化合物の解析より、mtmGI,mtmGIIがtetracyclic mithramycin前駆体のC-12a-OやmithramycinoneのC-2-Oへsugur moietyの異なる数をattachするmithramycin生合成中間体のグリコシル化を行うことを示した。

Sequence

>putative glycosyltransferase [putative glycosyltransferase]
MRIMFTASNWAGHYMCMVPLAWALRAAGHEIRVACPPSQERGVAATGLMPVPVLDAPDMM
ESARLAFYVQAMYTPPQSGPRPLPLHPFTGQPMQSLNDFDAGDLRDFWKKSIDAVQRSYD
NAVSFGDHWRPDLVVHDIMAVEGALVAALRGVPSVYVSPGFIGTVETEPGLDLVSADPLS
CFEKYGVEWGRDRIQYAVDPSPDVAVPPLGDALRLPMRYVPYNGAQGADPWQLGPIRGRR
VCIVWGNSASGIFGADVPALRHAIDAAVRQGAEVVLTAPQEQVEELGALPAGVRVLRNCP
LELILPYCDLLVHHGSANCYMNGIVAGIPQLSLALNYDTLICGRRIDPAGATLTLSGLEA
TAEKVEEALRRVLFDHRYRRAAEKLRDGVERAPSPAAVAGLLTRLVADGGLSAKDVAEVV
DNATEARRSA

>putative glycosyltransferase [putative glycosyltransferase]
ATGAGGATTATGTTCACCGCCTCGAACTGGGCGGGACATTACATGTGCATGGTGCCCCTG
GCCTGGGCGCTGCGCGCCGCGGGACACGAGATCCGGGTGGCGTGCCCGCCGTCGCAGGAA
CGCGGGGTCGCCGCGACCGGCCTGATGCCGGTCCCGGTGCTCGACGCGCCGGACATGATG
GAAAGCGCCCGGCTGGCGTTCTATGTGCAGGCCATGTACACACCGCCGCAGTCCGGTCCG
CGGCCGCTGCCGCTGCACCCGTTCACCGGGCAGCCGATGCAGTCGCTCAACGACTTCGAC
GCCGGCGACCTGCGCGACTTCTGGAAGAAGTCGATCGACGCGGTGCAGCGCAGCTATGAC
AACGCGGTGAGCTTCGGCGACCACTGGCGGCCCGACCTGGTGGTGCACGACATCATGGCC
GTCGAGGGCGCACTGGTCGCCGCGCTCCGGGGCGTGCCGAGCGTCTATGTGTCGCCCGGG
TTCATCGGCACCGTGGAGACCGAGCCGGGGCTCGACCTGGTCTCGGCGGACCCGCTGTCG
TGCTTCGAGAAGTACGGCGTCGAGTGGGGCCGCGACCGGATCCAGTACGCGGTGGACCCC
TCACCGGACGTGGCGGTGCCGCCGCTGGGCGACGCGCTGCGGCTGCCCATGCGCTACGTG
CCCTACAACGGCGCCCAGGGCGCGGACCCGTGGCAGCTGGGGCCGATCCGGGGCAGACGC
GTGTGCATCGTCTGGGGCAACTCGGCCTCGGGCATCTTCGGCGCCGACGTACCGGCGCTG
CGGCACGCCATCGACGCGGCGGTGCGGCAGGGCGCGGAGGTGGTGCTCACGGCGCCGCAG
GAACAGGTGGAGGAGCTGGGAGCGCTGCCGGCCGGCGTCCGGGTGCTGCGCAACTGCCCG
CTGGAGCTCATCCTCCCGTACTGCGACCTGCTGGTGCACCACGGCAGCGCCAACTGCTAC
ATGAACGGCATCGTGGCGGGGATTCCGCAGCTGTCCCTCGCCCTCAACTACGACACGCTG
ATCTGCGGCCGCCGTATCGATCCCGCCGGCGCCACCCTGACCCTGTCCGGCCTTGAGGCC
ACGGCCGAGAAGGTCGAGGAGGCGCTGCGCCGGGTGCTGTTCGACCACCGCTACCGCCGG
GCGGCGGAGAAACTGCGGGACGGCGTCGAGCGGGCACCTTCGCCCGCCGCGGTCGCCGGG
CTGCTGACCCGCCTCGTGGCGGACGGCGGGCTGTCGGCGAAGGACGTCGCCGAGGTCGTG
GACAACGCCACCGAGGCTCGGAGGAGCGCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002213 UDP-glucuronosyl/UDP-glucosyltransferase (Family) PF00201 UDPGT IPR004276 Glycosyl transferase, family 28 (Domain) PF03033 Glyco_transf_28 IPR010610 Domain of unknown function DUF1205 (Domain) PF06722 DUF1205
SignalP	Eukaryota Bacteria, Gram-positive Bacteria, Gram-negative
TMHMM	No significant hit

Avil_00260 Avil_00280