Avil_00250 Avil_00250   Avil_00270 Avil_00270

Avil_00260 : CDS information

close this sectionLocation

Organism
StrainTü57
Entry nameAvilamycin
Contig
Start / Stop / Direction25,761 / 26,432 / + [in whole cluster]
25,761 / 26,432 / + [in contig]
Location25761..26432 [in whole cluster]
25761..26432 [in contig]
TypeCDS
Length672 bp (223 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.3 uncharacterized protein (biosynthesis involved)
Producthypothetical protein
Product (GenBank)putative oxygenase
Gene
Gene (GenBank)aviO1
EC number
Keyword
Note
Note (GenBank)
  • AviO1
Reference
ACC
PmId
[11410376] Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics. (Chem Biol. , 2001)
[15640214] Genes involved in formation and attachment of a two-carbon chain as a component of eurekanate, a branched-chain sugar moiety of avilamycin A. (Appl Environ Microbiol. , 2005)
comment
[PMID:11410376]
Avilamycin生合成遺伝子クラスターの報告。

AviO1: L-proline 4-hydroxylase from Dactylosporangium sp. [Proposed function:C-O-C formation]

このORFの機能解析はされていない。


[PMID:15640214]
AviB1,AviB2,AviO1,AviO2,AviO3の機能解析の報告。

各欠損株を作製し、NMRにて構造解析を行っている。
aviO1, aviO2, aviO3は2-oxoglutarate-dependent enzymeをエンコードしていると推測され、avilamycin生合成においてC-C or O-C linkagesの形成に関与すると示唆された。
aviO1 or aviO3の不活性化はavilamycin生合成能が完全になくなり、培養液はantibiotic activityを全く示さなかった。したがって、avilamycin Aの生合成かあるいはその制御に関与していると推測された。機能は未知のままである。

close this sectionSequence

selected fasta
>hypothetical protein [putative oxygenase]
MTTATLDRAAAIERFRRDGFANAGPVLAPDAIARLKAGAERLITRFTDEGLRSDDYWNFP
VEGDERPVLYRVHNLEKQDWAPERDLLHREELAQLAAAFVDGPVVPTAYALVLKEPYRAA
EVPWHRDRVNVGPRTVCNLSICLDDAGPHNGCLEAVPGSHLLPDDAEVAKVRATGPVVPV
PVSQGDVVVHDVRLVHGSGPNANGSWRRTIVIEYADPAAPPAP
selected fasta
>hypothetical protein [putative oxygenase]
ATGACCACGGCGACACTGGACCGGGCCGCGGCGATCGAGCGGTTCCGACGGGACGGCTTC
GCGAACGCGGGCCCGGTTCTCGCCCCGGACGCGATCGCCCGTCTCAAGGCGGGCGCCGAA
CGGCTCATCACCCGCTTCACCGACGAGGGCCTGCGCTCCGACGACTACTGGAACTTCCCC
GTCGAGGGCGATGAGCGTCCCGTCCTCTACCGCGTGCACAACTTGGAGAAGCAGGACTGG
GCCCCGGAACGGGACCTGCTGCACCGCGAGGAACTGGCGCAGCTCGCCGCCGCGTTCGTG
GACGGCCCGGTCGTGCCCACCGCCTACGCGCTCGTCCTCAAGGAGCCGTACCGGGCCGCG
GAGGTGCCATGGCACCGTGACCGCGTCAACGTCGGCCCGCGCACGGTCTGCAATCTGAGC
ATCTGCCTGGACGACGCCGGACCGCACAACGGATGCCTGGAGGCGGTTCCGGGATCGCAC
CTGCTCCCCGACGACGCCGAGGTGGCGAAGGTGCGCGCGACCGGACCCGTGGTTCCGGTC
CCGGTCAGCCAGGGCGACGTGGTGGTGCACGACGTCCGGCTCGTCCACGGTTCGGGCCCG
AACGCCAACGGCAGTTGGCGGCGGACCATCGTCATCGAGTACGCCGACCCGGCCGCACCG
CCGGCGCCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR008775 Phytanoyl-CoA dioxygenase (Family)
[15-208] 1.2e-37 PF05721
PF05721   PhyH
SignalP No significant hit
TMHMM No significant hit
Avil_00250 Avil_00250   Avil_00270 Avil_00270
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