Avil_00240 Avil_00240   Avil_00260 Avil_00260

Avil_00250 : CDS information

close this sectionLocation

Organism
StrainTü57
Entry nameAvilamycin
Contig
Start / Stop / Direction25,011 / 25,610 / + [in whole cluster]
25,011 / 25,610 / + [in contig]
Location25011..25610 [in whole cluster]
25011..25610 [in contig]
TypeCDS
Length600 bp (199 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductD-fucose C-4 O-methyltransferase
Product (GenBank)putative methyltransferase
Gene
Gene (GenBank)aviG5
EC number2.1.1.-
Keyword
  • 4-O-methyl-D-fucose
Note
Note (GenBank)
  • AviG5
Reference
ACC
PmId
[11410376] Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics. (Chem Biol. , 2001)
[15489167] Novel avilamycin derivatives with improved polarity generated by targeted gene disruption. (Chem Biol. , 2004)
comment
[PMID:11410376]
avilamycin A生合成遺伝子クラスターの報告。

AviG5:Putative methyltransferase from Aeropyrum pernix K1 [Proposed function:Modification (methylation)]

AviG2,AviG3.AviG5,AviG6はavilamycin AのD-fucose moiety, D-mannose moiety, methyleurekanate moietyでメチル基を取り込むと推定している。
このORFの機能解析はされていない。


[PMID:15489167]
AviG2, AviG5, AviG6の機能解析の報告。

AviG5はS-adenosylmethionine-dependent methyltransferasesで保存されているmotif I,motif IIを保存していた。
TLC, HPLC-UV, HPLC/ESI-MSにて、aviG5のみの欠損株は1つのメチル基の欠如を、aviG4とaviG5の欠損株は2つのメチル基の欠如を、aviG4とaviG2とaviG5の欠損株は3つのメチル基の欠如を確認した。
また、NMR解析(HMQC,HMBC)では、AviG5がD-fucose moietyのposition 4のhydroxyl groupをメチル化することを明らかにした。

close this sectionSequence

selected fasta
>D-fucose C-4 O-methyltransferase [putative methyltransferase]
MNDLEIRRRVRELEPWVNDFVYNGVRYATASTRDYLLSQPPQERADAFFAAFPGAKRVLE
LGALEGADTLAMSGQPGVEILALEGREENLRRAEFVMEVHGVTNVELRLADVESMEFAGL
GHFDATLCAGLLYHVQRPWELLADIGSVSDCLYLSTHYWGSSDGMTDLDGTRSTPSAKTT
PSPRPAASASTCAGWTGRR
selected fasta
>D-fucose C-4 O-methyltransferase [putative methyltransferase]
GTGAACGATCTTGAGATTCGGCGTCGGGTACGTGAACTCGAGCCGTGGGTGAACGACTTT
GTGTACAACGGGGTCCGGTACGCCACCGCCTCCACGCGCGACTACCTGCTGAGCCAGCCG
CCCCAGGAGCGGGCCGACGCGTTCTTCGCGGCGTTCCCGGGGGCCAAGCGCGTCCTGGAG
CTCGGCGCGCTGGAGGGCGCGGACACCCTGGCGATGTCGGGACAGCCCGGCGTCGAGATC
CTCGCCCTGGAGGGCCGCGAAGAGAACCTGCGCCGCGCCGAGTTCGTCATGGAGGTGCAC
GGCGTCACCAACGTGGAACTCCGGCTCGCCGACGTGGAGTCGATGGAGTTCGCCGGGCTC
GGCCACTTCGACGCCACGCTCTGCGCAGGGCTCCTCTACCACGTGCAGCGGCCCTGGGAG
CTGCTCGCCGACATCGGCTCCGTCTCCGACTGCCTCTACCTCTCCACCCACTACTGGGGC
AGCTCGGACGGAATGACCGACCTCGACGGTACGCGGTCCACCCCGTCCGCGAAGACCACC
CCGAGCCCCAGGCCCGCGGCCTCAGCGTCGACGTGCGCTGGCTGGACCGGCCGTCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP
[1-30] 0.101 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Avil_00240 Avil_00240   Avil_00260 Avil_00260
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