Avil_00300 Avil_00300   Avil_00320 Avil_00320

Avil_00310 : CDS information

close this sectionLocation

Organism
StrainTü57
Entry nameAvilamycin
Contig
Start / Stop / Direction30,514 / 31,233 / + [in whole cluster]
30,514 / 31,233 / + [in contig]
Location30514..31233 [in whole cluster]
30514..31233 [in contig]
TypeCDS
Length720 bp (239 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductD-mannose C-2 O-methyltransferase
Product (GenBank)putative methyltransferase
Gene
Gene (GenBank)aviG6
EC number2.1.1.-
Keyword
  • 2,6-di-O-methyl-D-mannose
Note
Note (GenBank)
  • AviG6
Reference
ACC
PmId
[11410376] Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics. (Chem Biol. , 2001)
[15489167] Novel avilamycin derivatives with improved polarity generated by targeted gene disruption. (Chem Biol. , 2004)
comment
[PMID:11410376]
avilamycin A生合成遺伝子クラスターの報告。

AviG6:L-isoaspartate O-methyltransferase from A. pernix K1 [Proposed function:Modification (methylation)]

AviG2,AviG3.AviG5,AviG6はavilamycin AのD-fucose moiety, D-mannose moiety, methyleurekanate moietyでメチル基を取り込むと推定している。


[PMID:15489167]
AviG2, AviG5, AviG6の機能解析の報告。

AviG6はS-adenosylmethionine-dependent methyltransferasesで保存されているmotif Iを保存していた。
TLC, HPLC-UV, HPLC/ESI-MSにて、aviG4とaviG6の欠損株の培養上清は2つのメチル基の欠如を確認した。
また、NMR解析(HMQC,HMBC)では、AviG6がD-mannose moietyのposition 2のhydroxyl groupをメチル化することを明らかにした。

close this sectionSequence

selected fasta
>D-mannose C-2 O-methyltransferase [putative methyltransferase]
MPVPQLNDWYDQLTVEIIERVCAPDSGCLDIGAGGGEILTHMRRAAPRGRHFAVEPLPQY
AEQLRRDFPEVTVWQAAAAATPGRDSFVHVVSNPGYSGLRRRPYDRADETLEEIAVDTVR
LDDVVPEDARVDLVKVDVEGGEVGALRGAAELLRRQSPVVVFEHGGDHAMRDYGTTSDDL
WALLVDDLGYTLYTLPGWLAAEPGLDRAALAAELRTQWYFVADRGPLSARSPRGEVHST
selected fasta
>D-mannose C-2 O-methyltransferase [putative methyltransferase]
ATGCCCGTCCCCCAGCTCAACGACTGGTACGACCAGTTGACCGTCGAGATCATCGAACGG
GTCTGCGCCCCCGACTCCGGCTGCCTGGACATCGGTGCCGGCGGCGGCGAGATCCTCACG
CACATGCGCCGGGCCGCCCCGCGGGGCCGGCACTTCGCGGTCGAGCCGCTGCCGCAGTAC
GCCGAACAGCTGCGCCGCGACTTCCCCGAGGTCACGGTATGGCAGGCCGCCGCGGCCGCC
ACCCCTGGCCGGGACAGCTTCGTCCACGTGGTCTCCAACCCCGGCTACAGCGGCCTGCGC
CGCCGTCCGTACGACCGCGCCGACGAGACCCTGGAAGAGATCGCGGTGGACACCGTACGT
CTGGACGACGTCGTCCCCGAGGACGCCCGCGTGGACCTGGTCAAGGTGGACGTGGAGGGC
GGCGAGGTGGGCGCCCTGCGCGGGGCGGCCGAGCTGCTGCGCCGGCAGTCCCCCGTGGTG
GTCTTCGAACACGGCGGCGACCACGCGATGCGCGACTACGGCACCACCAGCGACGACCTG
TGGGCGCTGCTGGTCGACGACCTCGGCTACACGCTGTACACCCTGCCGGGCTGGCTGGCG
GCGGAGCCGGGCCTCGACCGGGCCGCTCTCGCCGCCGAGCTGCGCACGCAGTGGTACTTC
GTCGCCGATCGCGGGCCCCTGTCCGCCCGTTCCCCCCGAGGAGAAGTGCATTCGACATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006342 Methyltransferase FkbM (Domain)
[28-164] 9.5e-30 TIGR01444
TIGR01444   FkbM_fam
[30-192] 9.10000000000001e-22 PF05050
PF05050   Methyltransf_21
SignalP
[1-28] 0.066 Signal
Eukaryota   
TMHMM No significant hit
Avil_00300 Avil_00300   Avil_00320 Avil_00320
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