Avil_00460 : CDS information

Location

Organism	Streptomyces viridochromogenes
Strain	Tü57
Entry name	Avilamycin
Contig	AF333038
Start / Stop / Direction	45,278 / 46,327 / + [in whole cluster] 45,278 / 46,327 / + [in contig]
Location	45278..46327 [in whole cluster] 45278..46327 [in contig]
Type	CDS
Length	1,050 bp (349 aa)

Annotation

Category	3.2 modification methylation
Product	O-methyltransferase
Product (GenBank)
Gene	aviG4
Gene (GenBank)
EC number	2.1.1.-
Keyword	dichloroisoeverninic acid
Note
Note (GenBank)
Reference	PmId [11410376] Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics. (Chem Biol. , 2001) comment avilamycin A生合成遺伝子クラスターの報告。 AviG4:O-Demethylpuromycin O-methyltransferase from S. alboniger [Proposed function:Modification (methylation)] aviG4 knock out mutant、aviG4-aviH knock out double mutantを作製。検出された化合物の構造と、AviG4はO-demethylpuromycin methyltransferaseによく似ていることから、orsellinic acid moietyのmethylationに関与すると考察している。
Related Reference	ACC Q8KND2 NITE Calm_00650 PmId [18561189] Structural characterization of CalO2: a putative orsellinic acid P450 oxidase in the calicheamicin biosynthetic pathway. (Proteins. , 2009) comment Micromonospora echinospora CalO6_calO6 CalO1の機能解析論文。この本文中に、CalO6はin vitroでN-acetyl cysteaminyl orsellinic acid (SNAc-orsellinic acid)のC2 OH基を効率的にメチル化する(S. Singh & J. S. Thorson, unpublished work)、との記述がある。

Sequence

>O-methyltransferase
MAEKQAVAPPWTDLIRLVFGGMATQVVGLAVRLRLPDAIGEGERTADGLAADFESEPAAM
NRLLRGLAALGVLRESKPGVFALTPVGELRRADRSPSFHSLARMLTDPAVATAWQHLDHS
VRTGGPAFDHVFGRDFFAYLADDPDLSWLYNAAMSQGTGGIAGLVAAHQDFSGVRTVVDV
GGGDGTLLAAVLRAHPSLRGVLYDSAAGVAQAGEVLAAAGVADRAVEAGDFFAAVPEGGD
LYLLKSVVHGWEDERAARILAHCRRALPAHGRIVMVEHLLPDTVPADAVPTTYLNDLNLL
VNGNGLERTRGDFEQLCAAAGLTVEAVTPLAGTDLWLIEAVPASADPLG

>O-methyltransferase
ATGGCGGAGAAGCAGGCCGTGGCGCCCCCCTGGACGGACCTGATCCGGCTGGTGTTCGGC
GGAATGGCCACCCAGGTCGTGGGCCTGGCGGTGCGGCTGCGGCTGCCCGACGCGATCGGG
GAGGGCGAACGGACCGCCGACGGGCTGGCCGCCGACTTCGAGAGCGAACCCGCTGCCATG
AACCGGCTGTTGCGCGGCCTGGCCGCGCTCGGAGTGCTGCGCGAGTCCAAGCCCGGCGTG
TTCGCCCTGACACCGGTCGGGGAACTGCGTCGCGCCGACCGCTCGCCGTCCTTCCACTCA
CTGGCCCGGATGCTCACCGATCCCGCGGTCGCGACGGCCTGGCAGCACCTGGACCACAGC
GTCCGCACCGGCGGCCCGGCCTTCGACCACGTCTTCGGCCGGGACTTCTTCGCGTACCTG
GCGGACGACCCCGACCTGTCGTGGCTCTACAACGCGGCGATGAGCCAGGGCACCGGTGGG
ATCGCCGGCCTGGTCGCGGCGCACCAGGACTTCTCCGGCGTCCGCACCGTGGTGGACGTC
GGGGGCGGGGACGGGACGCTGCTGGCCGCGGTGCTGCGCGCGCACCCCTCGCTGCGCGGC
GTGCTGTACGACTCGGCGGCCGGGGTCGCGCAGGCCGGGGAGGTGCTGGCGGCGGCGGGC
GTCGCGGACCGCGCGGTGGAGGCCGGCGACTTCTTCGCCGCCGTACCCGAGGGCGGTGAC
CTCTACCTGCTCAAGAGCGTCGTCCATGGCTGGGAGGACGAGCGGGCCGCGAGGATTCTG
GCGCACTGCCGCCGGGCACTGCCGGCGCACGGCCGGATCGTCATGGTCGAGCACCTGCTG
CCCGACACGGTGCCCGCGGACGCCGTGCCGACGACCTACCTCAACGACCTCAACCTGCTG
GTCAACGGCAACGGGCTGGAGCGCACGCGTGGCGACTTCGAACAGCTCTGCGCGGCGGCG
GGCCTGACGGTCGAAGCGGTCACGCCGCTGGCCGGAACCGACCTGTGGCTGATCGAGGCC
GTGCCCGCTTCGGCTGACCCGCTCGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001077 O-methyltransferase, family 2 (Domain) PF00891 Methyltransf_2 IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 no description
SignalP	No significant hit
TMHMM	No significant hit

Avil_00450 Avil_00475