Avil_00475 : CDS information

Location

Organism	Streptomyces viridochromogenes
Strain	Tü57
Entry name	Avilamycin
Contig	AF333038
Start / Stop / Direction	46,609 / 47,556 / + [in whole cluster] 46,609 / 47,556 / + [in contig]
Location	46609..47556 [in whole cluster] 46609..47556 [in contig]
Type	CDS
Length	948 bp (315 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative GDP-mannose 4,6-dehydratase putative GDP-D-mannose dehydratase
Product (GenBank)
Gene	aviE3 gmd
Gene (GenBank)
EC number	4.2.1.47
Keyword
Note
Note (GenBank)
Reference	PmId [11410376] Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics. (Chem Biol. , 2001) comment avilamycin A生合成遺伝子クラスターの報告。 AviE3: dGDP-mannose-4,6-dehydratase from S. noursei ATCC 11455 [Proposed function:Sugar biosynthesis (4-O-methyl-D-fucose)] AviE3はGDP-D-fucose biosynthesisの最初の中間体としてGDP-mannose→GDP-4-keto-6-deoxy-D-mannoseの反応に関与するかもしれないと推定している。このORFの機能解析はされていない。
Related Reference	ACC Q9L4X4 NITE Nysta_00040 PmId [17905880] Analysis of the mycosamine biosynthesis and attachment genes in the nystatin biosynthetic gene cluster of Streptomyces noursei ATCC 11455. (Appl Environ Microbiol. , 2007) comment Streptomyces noursei NysDIII_nysDIII Nystatinのmycosamine moiety生合成遺伝子 NysDIII がGDP-mannose→GDP-4-keto-6-deoxy-D-mannoseへ変換することを反応産物のHPLCで確認している。

Sequence

>putative GDP-mannose 4,6-dehydratase
MVRGQGRPYARDGRPLHPDIRVVSGDLLDQTSLIAAVEQSCPTEIYNLGALSFVPVSWKQ
PTVAAEVTGKGVLRMLEAIRITAGLSSSRTTSSGVPRFYQASTSEMFGKVRESPQSEATP
FHPRSPYGVAKAFGHYMVQNYRESYGMFAVSGILFNHESPIRGPEFVTRKVSLGVAAVKL
GLMDKLRLGNLEAERDWGFAGDYVRGMTMMLAQDEPEDLVLGTGITHRVRDLVDFAFAHV
GLDWRDHVEVDSRLLRPAEVELLCADLSRARQKLGWKPEVSFEELVAMMVDSDLRLLTDS
EDAAGEAVLEQAGLW

>putative GDP-mannose 4,6-dehydratase
ATGGTGCGCGGGCAGGGCCGGCCGTATGCCCGCGACGGTCGGCCCCTGCACCCGGACATC
CGAGTGGTGAGCGGCGACTTGCTGGACCAGACGAGCCTGATCGCTGCTGTCGAGCAGTCC
TGCCCCACCGAGATCTACAACCTCGGCGCCCTGTCGTTCGTCCCGGTCTCCTGGAAGCAG
CCCACCGTTGCCGCGGAGGTGACCGGCAAGGGTGTGCTGCGGATGCTGGAGGCCATCCGC
ATCACGGCGGGGCTCAGCAGTTCCCGGACCACCAGCAGCGGCGTCCCGCGCTTCTATCAG
GCCTCCACCTCGGAGATGTTCGGAAAGGTGCGGGAGTCCCCGCAGAGCGAGGCCACGCCC
TTCCACCCGCGCAGCCCCTACGGGGTGGCCAAGGCGTTCGGGCACTACATGGTGCAGAAC
TACCGCGAGTCGTACGGGATGTTCGCGGTCAGCGGGATCCTGTTCAACCACGAGTCACCG
ATCCGGGGCCCGGAGTTCGTCACGCGCAAGGTGTCGCTCGGGGTGGCCGCCGTGAAGCTG
GGCCTGATGGACAAGCTCCGGCTGGGCAACCTGGAGGCGGAACGCGACTGGGGCTTCGCA
GGGGACTACGTGCGCGGCATGACGATGATGCTCGCCCAGGACGAGCCGGAGGACCTCGTC
CTCGGCACCGGGATCACCCACCGGGTGCGCGATCTGGTGGATTTCGCCTTCGCGCACGTC
GGGCTGGACTGGCGCGACCACGTGGAGGTGGACTCCCGGCTGCTGCGCCCGGCGGAGGTG
GAACTGCTCTGCGCCGACCTGAGCCGCGCTCGGCAGAAGCTCGGCTGGAAGCCCGAGGTC
TCGTTCGAGGAACTGGTCGCCATGATGGTCGACAGCGACCTGCGCCTGCTCACCGACAGT
GAGGACGCCGCCGGCGAGGCCGTTCTCGAACAGGCCGGTCTGTGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 no description
SignalP	No significant hit
TMHMM	No significant hit

Avil_00460 Avil_00480