Bena_00060 Bena_00060   Bena_00080 Bena_00080

Bena_00070 : CDS information

close this sectionLocation

Organism
StrainA2991200
Entry nameBenastatin
Contig
Start / Stop / Direction6,112 / 6,432 / + [in whole cluster]
6,112 / 6,432 / + [in contig]
Location6112..6432 [in whole cluster]
6112..6432 [in contig]
TypeCDS
Length321 bp (106 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.4 other modification
Productputative cyclase
Product (GenBank)BenE protein
Gene
Gene (GenBank)benE
EC number
Keyword
  • pentangular
Note
Note (GenBank)
Reference
ACC
PmId
[17439117] Molecular analysis of the benastatin biosynthetic pathway and genetic engineering of altered fatty acid-polyketide hybrids. (J Am Chem Soc. , 2007)
comment
Benastatin A and B生合成をコードする全遺伝子座のクローニング、シークエンス。
flanking regionsの削除とS. albus, S. lividansでの異種性発現によってclusterが同定されている。

BenE: cyclase I

配列解析のみ。
GrhQ, FdmDにとても似ている。
Related Reference
ACC
P39890
PmId
[8218177] ( , )
comment
Blast 14th, id54%, 2e-24
Streptomyces glaucescens_tcmI
Tetracenomycin polyketide synthesis protein tcmI

abstract:
Tetracenomycin (Tcm) F2 cyclaseはtcmI にコードされる。
gel-filtration chromatographyより、溶液中でhomotrimer形成と判明。

pH8.0以上で、TcmI はanthrone Tcm F2 → naphthacenone Tcm F1への環化を触媒。
これは4つある環の最後の1つのみの環化。
Km of 121 +/- 18.2 microM and Vmax of 704 +/- 62.3 nmol.min-1.mg-1

pH6.5以下では、Tcm F2 → 9-decarboxy Tcm F1(Tcm-C生合成経路のshunt産物)への環化を触媒。

close this sectionSequence

selected fasta
>putative cyclase [BenE protein]
MHRTLIVARMKPEDADKVADLFRWSDSTELPHLIGARQRTLFRFHDLYMHLVEADEDPTQ
KLYAAREHPLFKELHERLLPFITAYDPKWSEPKHAMALPFYTWQAD
selected fasta
>putative cyclase [BenE protein]
ATGCACCGCACTTTGATCGTGGCGCGGATGAAACCCGAAGACGCCGACAAGGTCGCCGAC
CTGTTCCGCTGGTCGGACAGCACGGAACTGCCGCATCTCATCGGCGCACGGCAACGCACG
CTCTTCCGCTTCCACGACCTGTACATGCACCTCGTGGAGGCCGACGAGGACCCCACGCAG
AAACTGTACGCGGCACGGGAGCACCCGCTCTTCAAGGAGCTCCACGAGAGGCTGCTGCCA
TTCATCACCGCGTACGACCCCAAGTGGTCCGAGCCCAAGCACGCCATGGCGCTGCCGTTC
TACACCTGGCAGGCCGACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006765 Polyketide synthesis cyclase (Family)
[2-103] PD012644
PD012644   Polyketide_synth_cyclase
[1-105] 2.8e-47 G3DSA:3.30.70.1090
G3DSA:3.30.70.1090   Cyclase_polyket
[10-105] 2.70000000000002e-36 PF04673
PF04673   Cyclase_polyket
IPR011008 Dimeric alpha-beta barrel (Domain)
[1-106] 1.70000295590053e-44 SSF54909
SSF54909   Dimer_A_B_barrel
SignalP No significant hit
TMHMM No significant hit
Bena_00060 Bena_00060   Bena_00080 Bena_00080
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