Chalc_00010 : CDS information

Location

Organism	Streptomyces bikiniensis
Strain	NRRL 2737
Entry name	Chalcomycin
Contig	AY509120
Start / Stop / Direction	1,009 / 2 / - [in whole cluster] 1,009 / 2 / - [in contig]
Location	complement(2..1009) [in whole cluster] complement(2..1009) [in contig]
Type	CDS
Length	1,008 bp (335 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein(fragment)
Product (GenBank)	conserved hypothetical protein
Gene
Gene (GenBank)	ORF1
EC number
Keyword
Note	It is supposed that this ORF is not involved in chalcomycin biosynthesis.
Note (GenBank)	similar to Streptomyces coelicolor SCO0450
Reference	ACC Q5SFA2 PmId [15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004) comment Chalcomycin biosynthesis gene clusterに関する文献。 ORF1(343a.a.):membrane protein ORF1自体の機能実験は無し。（論文のTable 2. ではアミノ酸サイズが343となっているが、OCSSでは335 a.a.で、途中の配列まで。UniProt登録のQ5SFA2も配列は同じく途中まで）

Sequence

>hypothetical protein(fragment) [conserved hypothetical protein]
MADVLDIRQYRSPAATALMSLAVAVLYYAAGRLGLMGRLVVEGVVVTPIWPPTGVAVAAL
LLLGARVWPGIALGSFLVIASLTTPGPTTVVTVVSNTVAPLCAFLLLTRAGFRLDMARLR
DGLSLVFLGGFGAMLISATAGVGLQVAKSSLDTTEFWPVWLAWWVGDTMGVLLVAPLLLV
LAGPAGRFRVRRWKEAALLGLATLILMPMAVLSPVGMLFLVFPLLIWAALRFQLTGSMLC
ALFASVLATFEANAGRGGFLHLSDVEIMAKLQAFNGSAALTALLLASVITEQRATRRSVR
RACRELAEVLEHLAAGEPAPGPPGTAESRAAPSGGP

>hypothetical protein(fragment) [conserved hypothetical protein]
ATGGCAGACGTGTTGGATATTCGGCAGTACCGGTCCCCCGCGGCGACGGCGCTGATGTCG
CTGGCCGTGGCGGTGCTCTACTACGCGGCCGGACGACTCGGCCTGATGGGCCGCCTCGTC
GTCGAAGGCGTGGTGGTCACCCCCATCTGGCCTCCCACCGGCGTCGCCGTCGCCGCCCTG
CTGCTGCTCGGCGCGCGCGTCTGGCCCGGGATCGCCCTCGGCTCCTTCCTCGTCATCGCC
TCCCTCACCACGCCGGGGCCCACCACCGTGGTCACCGTGGTCAGCAACACCGTCGCGCCG
CTCTGCGCCTTCCTGCTGCTGACACGGGCCGGCTTCCGGCTCGACATGGCACGGCTGCGG
GACGGACTCTCCCTGGTCTTCCTCGGCGGGTTCGGCGCCATGCTGATCAGCGCGACCGCC
GGGGTCGGACTGCAGGTGGCGAAGAGCTCCCTGGACACGACCGAGTTCTGGCCCGTCTGG
CTGGCCTGGTGGGTGGGCGACACGATGGGGGTGCTGCTCGTCGCCCCGCTCCTCCTCGTC
CTCGCGGGGCCGGCCGGACGGTTCCGCGTACGGCGCTGGAAGGAGGCGGCCCTGCTGGGT
CTCGCGACCCTGATCCTCATGCCCATGGCCGTGCTCAGCCCGGTGGGCATGCTCTTCCTC
GTCTTCCCCCTGCTGATCTGGGCGGCGCTCCGCTTCCAGCTCACCGGAAGCATGCTCTGC
GCGCTCTTCGCCTCCGTGCTCGCCACCTTCGAGGCGAACGCCGGACGGGGAGGGTTCCTC
CACCTCTCGGACGTCGAGATCATGGCCAAGCTCCAGGCGTTCAACGGTTCCGCCGCACTG
ACCGCCCTGCTTCTCGCCTCCGTCATCACGGAGCAGCGTGCGACCCGCCGCTCGGTACGC
CGCGCCTGCCGGGAACTGGCGGAGGTCCTGGAGCATCTCGCCGCGGGCGAGCCCGCACCC
GGCCCACCGGGCACCGCCGAGAGCCGGGCAGCCCCGTCCGGCGGGCCC

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR007895 MASE1 (Domain) PF05231 MASE1
SignalP	Eukaryota
TMHMM	Transmembrane 8

first Chalc_00020