Chalc_00020 : CDS information

Location

Organism	Streptomyces bikiniensis
Strain	NRRL 2737
Entry name	Chalcomycin
Contig	AY509120
Start / Stop / Direction	1,963 / 1,121 / - [in whole cluster] 1,963 / 1,121 / - [in contig]
Location	complement(1121..1963) [in whole cluster] complement(1121..1963) [in contig]
Type	CDS
Length	843 bp (280 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	conserved hypothetical protein
Gene
Gene (GenBank)	ORF2
EC number
Keyword
Note	It is supposed that this ORF is not involved in chalcomycin biosynthesis.
Note (GenBank)	similar to Streptomyces coelicolor SCO0451
Reference	ACC Q5SFA1 PmId [15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004) comment Chalcomycin biosynthesis gene clusterに関する文献。 ORF2(280a.a.):hypothetical protein ORF2自体の機能実験は無し。

Sequence

>hypothetical protein [conserved hypothetical protein]
MFRRRPADSSEDLLVRLGSLTARARELAETQRSRVELAVALQRGMLPSDLPSLPGARLAV
RYEPANHGLNVGGDWYDAFPLPGGRIGMSIGDVQGHNIEAAAFMGQVRVALRALASVTAD
PGELLGRTNDLLVSLGADLFATCTFLRLDPAAGTLECARAGHIPHIWATADGRSGIDDSE
GGPPLGVLRGADYPATRHRLTADGVFVLLTDGVVEGPSLHIDEGLDRVTRLAGITAVAGL
DVDALATAVMRLAATVGHEDDAAVLVVGHDGGPPRPATGE

>hypothetical protein [conserved hypothetical protein]
ATGTTCCGCAGACGCCCGGCCGACAGCAGCGAGGACCTTCTGGTCCGGCTCGGGTCGCTG
ACCGCCAGGGCGCGGGAGCTGGCCGAGACGCAGCGGTCCCGTGTCGAGCTCGCCGTGGCC
CTGCAGCGCGGCATGCTGCCCTCGGACCTGCCCAGTCTTCCGGGTGCCCGGCTGGCCGTC
CGGTACGAGCCCGCCAACCACGGGCTCAACGTCGGCGGCGACTGGTACGACGCCTTCCCC
CTGCCTGGCGGGCGGATCGGCATGTCCATCGGCGACGTCCAGGGACACAACATCGAGGCC
GCCGCCTTCATGGGACAGGTCCGCGTGGCACTGCGCGCGCTGGCCTCCGTCACCGCGGAC
CCGGGAGAGCTGCTCGGCCGCACCAACGACCTGCTCGTCTCCCTCGGCGCCGACCTCTTC
GCCACCTGCACCTTCCTGCGGCTCGACCCCGCCGCCGGCACCCTGGAATGCGCCCGGGCC
GGCCACATCCCCCACATCTGGGCCACCGCCGACGGCCGCTCCGGCATCGACGACAGCGAG
GGCGGGCCTCCTCTCGGCGTGCTGCGCGGCGCCGACTACCCGGCCACGCGCCACCGGCTC
ACCGCCGACGGCGTCTTCGTCCTCCTGACCGACGGGGTCGTGGAGGGGCCCTCGCTCCAC
ATCGACGAGGGTCTCGACCGGGTGACGAGACTCGCCGGGATCACGGCCGTCGCCGGGCTG
GACGTCGACGCGCTCGCCACCGCCGTCATGCGACTGGCGGCCACGGTGGGGCACGAGGAC
GACGCGGCCGTCCTCGTCGTCGGCCACGACGGCGGTCCGCCCCGGCCGGCGACCGGGGAG
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001932 Protein phosphatase 2C-like (Domain) SM00331 PP2C_SIG PF07228 SpoIIE
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Chalc_00010 Chalc_00030