Chalc_00030 : CDS information

Location

Organism	Streptomyces bikiniensis
Strain	NRRL 2737
Entry name	Chalcomycin
Contig	AY509120
Start / Stop / Direction	3,889 / 3,008 / - [in whole cluster] 3,889 / 3,008 / - [in contig]
Location	complement(3008..3889) [in whole cluster] complement(3008..3889) [in contig]
Type	CDS
Length	882 bp (293 aa)

Annotation

Category	5.1 general function
Product	putative oxidoreductase
Product (GenBank)	conserved hypothetical protein
Gene
Gene (GenBank)	ORF3
EC number
Keyword
Note	It is supposed that this ORF is not involved in chalcomycin biosynthesis.
Note (GenBank)	putative oxidoreductase; similar to Streptomyces coelicolor SCO0299, Mycobacterium leprae ML0458, and Deinococcus radiodurans DR1890
Reference	ACC Q5SFA0 PmId [15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004) comment Chalcomycin biosynthesis gene clusterに関する文献。 ORF3(293a.a.):oxidoreductase ORF3自体の機能実験は無し。
Related Reference	ACC Q9X5G7 PmId [10223939] Genes specific for the biosynthesis of clavam metabolites antipodal to clavulanic acid are clustered with the gene for clavaminate synthase 1 in Streptomyces clavuligerus. (Antimicrob Agents Chemother. , 1999) comment 391st(Q9X5G7) 39%, 5e-38 Streptomyces clavuligerus ATCC 27064_cvm1 Putative aldo/keto reductase family 2 enzyme beta-lactamase inhibitorであるClavulanic acid 生合成に関与するclavaminate synthase 1 isoenzyme (CAS1)のシーケンス決定と特徴づけ。 cvm1はcas1のすぐ上流に存在しており、cvm1にapramycin resistance gene (apr) cassetteを変換して欠損株も作っている。cmvのアミノ酸配列はaldo and keto reductase family 2 enzymesに属するアミノ酸と相同性が見られた。 cvm1 mutant産物はHPLCによって確認され、clavam-2-carboxylate, clavaminic acid, 2-hydroxymethylclavamは検出されず、clavulanic acid産生のみが得られた。
Family/Domain	FD IPR020471 comment [IPR020471] Aldo/keto reductase subgroup (Family) function: oxidoreductase activity

Sequence

>putative oxidoreductase [conserved hypothetical protein]
MAQPGRTTFPLGGDLSVHRLGFGALRLTGPGYWGPPEDREAAISTARLAVDLGVSFIDTA
DSYGLGSNEELLAEALRPYPDGLVIATKAGQCRPRPDQWVPCGRPEYLRQQAELSLRRLR
LDRIDLFQLHRIDPKVPAAEQFGALGELQDEGKIRHIGLSKVTVREVEAAREHITVASVQ
NVYNLTDRRNDEVVEYCEKHEIAFIAWLPIARGALAAGGSPVADIATELEVSAGQVALAW
LLHRSPAVIPIPGTSSPAHLRENVAASGIALSDGQLARLNALGRTHDDATVPV

>putative oxidoreductase [conserved hypothetical protein]
ATGGCACAGCCCGGCAGGACCACCTTCCCTCTGGGAGGCGACCTCTCGGTACACCGGTTG
GGTTTCGGCGCCCTCCGGCTGACCGGTCCGGGGTACTGGGGCCCGCCGGAGGACCGGGAG
GCTGCGATCAGCACGGCCCGGCTGGCCGTGGACCTCGGTGTGTCCTTCATCGACACGGCC
GATTCCTACGGGCTGGGAAGCAACGAGGAGCTGCTGGCCGAAGCACTGCGCCCGTACCCC
GACGGCCTCGTGATCGCGACGAAGGCCGGACAGTGCCGGCCCCGTCCCGACCAGTGGGTG
CCGTGCGGCCGACCGGAGTACCTCCGCCAGCAGGCCGAGCTGAGCCTGCGCAGGCTGCGA
CTCGACCGCATCGACCTCTTCCAGCTGCACCGGATCGATCCGAAGGTGCCGGCGGCCGAG
CAGTTCGGTGCGCTCGGGGAACTGCAGGACGAAGGGAAGATCCGGCACATCGGGCTGTCC
AAGGTCACCGTCCGGGAAGTGGAGGCCGCCCGTGAGCACATCACCGTGGCCAGCGTGCAG
AACGTGTACAACCTGACCGACCGGCGCAACGACGAGGTGGTCGAGTACTGCGAGAAGCAC
GAGATCGCGTTCATCGCCTGGCTGCCGATCGCCCGCGGTGCGCTCGCCGCCGGGGGGAGC
CCGGTGGCCGACATCGCCACCGAGCTGGAGGTGTCGGCCGGTCAGGTGGCGCTCGCGTGG
CTGCTGCACCGCTCGCCGGCGGTCATTCCCATCCCCGGCACCAGCTCGCCCGCGCATCTG
AGGGAGAACGTCGCGGCGTCGGGCATCGCTCTCTCCGATGGCCAGCTGGCCCGGCTGAAC
GCGCTCGGACGCACGCACGACGACGCCACGGTGCCCGTGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR020471 Aldo/keto reductase subgroup (Family) PR00069 ALDKETRDTASE IPR023210 NADP-dependent oxidoreductase domain (Domain) G3DSA:3.20.20.100 Aldo/ket_red SSF51430 Aldo/ket_red PF00248 Aldo_ket_red
SignalP	No significant hit
TMHMM	No significant hit

Chalc_00020 Chalc_00040