Chalc_00030 Chalc_00030   Chalc_00050 Chalc_00050

Chalc_00040 : CDS information

close this sectionLocation

Organism
StrainNRRL 2737
Entry nameChalcomycin
Contig
Start / Stop / Direction5,208 / 3,991 / - [in whole cluster]
5,208 / 3,991 / - [in contig]
Locationcomplement(3991..5208) [in whole cluster]
complement(3991..5208) [in contig]
TypeCDS
Length1,218 bp (405 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose C-4 aminotransferase
Product (GenBank)putative D-chalcose pathway component
Gene
Gene (GenBank)chmCIV
EC number2.6.1.-
Keyword
  • D-chalcose
Note
Note (GenBank)
  • predicted to participate with ChmCV in production of 4-deoxy in D-chalcose pathway; similar to EryCIV, OleN1
Reference
ACC
PmId
[15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004)
comment
Chalcomycin biosynthesis gene clusterに関する文献。

chmCIV(405a.a.): 3,4-DH; D-chalcose pathway

chmCIV自体の機能実験は行われておらず、erythromycinやpikromycin生合成経路におけるeryCIVやdesIとのホモログであることから、chmCVと共にNDP-chalcose生産のためのC-4におけるdeoxygenationへの関与が予測されている。
Related Reference
ACC
Q331S0
PmId
[17532307] Enzymatic synthesis of dTDP-4-amino-4,6-dideoxy-D-glucose using GerB (dTDP-4-keto-6-deoxy-D-glucose aminotransferase). (Carbohydr Res. , 2007)
comment
2nd(Q331S0) 93%, 0.0
Streptomyces sp. KCTC 0041BP_gerB
3,4-dehydratase-like protein

Streptomyces sp., GERI-155 = Streptomyces sp. KCTC 0041BP

5つの酵素がglucose-1-phosphate and TMPから大規模にdTDP-4-amino-4,6-dideoxy-D-glucoseを合成するために使用された。
GerB (dTDP-4-keto-6-deoxy-D-glucose aminotransferase)はdTDP-4-keto-6-deoxy-D-glucoseからのdTDP-4-amino-4,6-dideoxy-D-glucoseの酵素的合成において使用された。
ACC
Q9ZGH0
NITE
Pikro_00160
PmId
[17630700] Molecular architecture of DesI: a key enzyme in the biosynthesis of desosamine. (Biochemistry. , 2007)
comment
9th(Q9ZGH0) 64%, 1e-145
Streptomyces venezuelae_desI
[DoBISCUIT]putative NDP-hexose C-4 aminotransferase

PLP-dependent aminotransferase_DesIのX線結晶構造解析。
DesIをその反応産物であるdTDP-4-amino-4,6-dideoxyglucoseとの複合体で解析。
DesIと同様に産物のdideoxyglucosyl基とタンパクとの間の水素結合相互作用がないHelicobacter pylori_PseCとの構造比較から、転移する糖の向きの違いを説明している。

close this sectionSequence

selected fasta
>putative NDP-hexose C-4 aminotransferase [putative D-chalcose pathway component]
MTMKRNLGDLAVFGGPSAFLQPLHVGRPNVGDRARFLDRVNWALDHQWLTNGGPLAREFE
QRVAELAGVRYCVSTCNATVALQLLARAADLSGEVIMPSLTFPATAQAFRWLGLTPVFVD
IDPATNCLDPDLIEAAITPRTSAVVPVHLWGRPCAVDRLAKVAADHGLRLFYDAAHGLGC
TSEGQPIGGFGQAEVFSFHATKVVNAFEGGAVVTDDERLAQRVRAMHNFGFTQGRVSTET
GTNGKMTEAAAAMGLTSLDAFEETVRRNEANHDDYRSELAGLAGLKVMEFDRAERNNYHY
LVVEVDAAVTGVHRDLLDEVLRAENIMCQRYFSPGCHEMEPYRSERPVSLPHTERLARKV
LALPTGPTVSREDIRRVCDIVRLTLERGHEVTRRADERVPSTTAP
selected fasta
>putative NDP-hexose C-4 aminotransferase [putative D-chalcose pathway component]
ATGACCATGAAACGCAATCTCGGTGATCTCGCCGTGTTCGGCGGCCCGTCGGCGTTCCTG
CAACCACTTCACGTGGGGCGGCCCAACGTGGGCGACCGAGCGCGGTTCCTGGACCGTGTG
AACTGGGCGCTGGACCACCAGTGGCTGACCAACGGCGGGCCGCTGGCCCGGGAGTTCGAG
CAGCGCGTGGCGGAACTCGCCGGCGTGCGCTACTGCGTGTCGACCTGCAACGCGACCGTC
GCCCTCCAGTTGCTGGCCCGCGCCGCCGATCTGTCGGGCGAGGTCATCATGCCGTCGCTG
ACCTTCCCGGCGACCGCCCAGGCGTTCCGCTGGCTGGGTCTGACCCCGGTCTTCGTCGAC
ATCGACCCGGCCACCAACTGCCTGGACCCGGATCTGATCGAGGCGGCGATCACCCCCCGT
ACGAGTGCGGTCGTGCCGGTCCATCTGTGGGGTCGGCCCTGCGCGGTCGACCGGCTGGCG
AAGGTCGCGGCCGATCACGGTCTCAGGCTCTTCTACGATGCCGCGCACGGACTCGGCTGC
ACGTCGGAAGGGCAGCCGATCGGCGGCTTCGGACAAGCCGAGGTGTTCAGCTTCCACGCC
ACCAAGGTGGTCAACGCCTTCGAGGGCGGCGCGGTGGTGACCGATGACGAGAGGCTGGCC
CAGCGGGTCCGCGCGATGCACAACTTCGGATTCACCCAGGGGCGGGTGTCCACGGAGACC
GGCACCAACGGGAAGATGACCGAAGCCGCCGCCGCGATGGGACTCACCTCGCTCGACGCC
TTCGAGGAGACGGTTCGCCGGAACGAGGCCAACCACGACGACTACCGGTCGGAACTCGCC
GGGCTCGCCGGGCTGAAGGTCATGGAGTTCGACCGTGCCGAGCGCAACAACTACCACTAT
CTGGTCGTGGAGGTCGACGCCGCGGTGACCGGGGTCCACCGGGACCTGCTGGACGAGGTG
CTGAGGGCGGAGAACATCATGTGCCAGCGGTACTTCTCCCCCGGATGCCACGAGATGGAG
CCCTACCGCTCGGAGCGGCCGGTCTCCCTGCCCCACACCGAACGGCTCGCGCGGAAGGTC
CTCGCCCTGCCCACCGGCCCCACCGTCTCTCGCGAGGACATCCGTCGGGTCTGCGACATC
GTGCGGCTCACTCTGGAGCGGGGACACGAGGTGACCCGACGGGCCGACGAGCGGGTGCCC
TCCACGACGGCTCCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000653 DegT/DnrJ/EryC1/StrS aminotransferase (Family)
[2-389] PIRSF000390
PIRSF000390   PLP_StrS
[34-382] 2.50000000000001e-106 PF01041
PF01041   DegT_DnrJ_EryC1
IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain)
[23-259] 2.09999999999996e-69 G3DSA:3.40.640.10
G3DSA:3.40.640.10   PyrdxlP-dep_Trfase_major_sub1
IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain)
[262-387] 1.3e-28 G3DSA:3.90.1150.10
G3DSA:3.90.1150.10   PyrdxlP-dep_Trfase_major_sub2
IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain)
[21-386] 1.90000694315261e-90 SSF53383
SSF53383   PyrdxlP-dep_Trfase_major
SignalP No significant hit
TMHMM No significant hit
Chalc_00030 Chalc_00030   Chalc_00050 Chalc_00050
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