Chalc_00050 : CDS information

Location

Organism	Streptomyces bikiniensis
Strain	NRRL 2737
Entry name	Chalcomycin
Contig	AY509120
Start / Stop / Direction	6,118 / 5,339 / - [in whole cluster] 6,118 / 5,339 / - [in contig]
Location	complement(5339..6118) [in whole cluster] complement(5339..6118) [in contig]
Type	CDS
Length	780 bp (259 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose O-methyltransferase
Product (GenBank)	putative O-methyltransferase in D-chalcose pathway
Gene
Gene (GenBank)	chmCI
EC number	2.1.1.-
Keyword	D-chalcose
Note
Note (GenBank)	similar to SpnH
Reference	ACC Q5SF98 PmId [15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004) comment Chalcomycin biosynthesis gene clusterに関する文献。 chmCI(259a.a.): O-methyltransferase; D-chalcose pathway chmCI 自体の機能実験は行われておらず、spinosynのrhamnose precursorのO methylationに関与するspnHとの相同性の高さより、NDP-4,6-dideoxy-D-glucoseの3-O-methylationを触媒し、NDP-chalcoseを生じると予想している。
Related Reference	ACC Q9ALM9 NITE Spino_00140 PmId [20158237] Biosynthesis of spinosyn in Saccharopolyspora spinosa: synthesis of permethylated rhamnose and characterization of the functions of SpnH, SpnI, and SpnK. (J Am Chem Soc. , 2010) comment 6th(Q9ALM9) 59%, 6e-79 Saccharopolyspora spinosa_spnH [Spino_00140]rhamnose 4'-O-methyltransferase rhamnose methyltransferasesとされるSpnH, SpnI, and SpnKの機能検証、そのpermethylation過程の役割を確認。 SpnH, SpnI, and SpnK がそれぞれrhamnose 4'-, 2'-, and 3'-O-methyltransferaseであることが確立された。これらの共通した基質は、rhamnoseが付加されたaglyconeである。また、SpnI→SpnK→SpnHという順番で働くこともわかっている。
Related Reference	ACC Q9AJU0 NITE Ello_00200 PmId [11376004] Deoxysugar methylation during biosynthesis of the antitumor polyketide elloramycin by Streptomyces olivaceus. Characterization of three methyltransferase genes. (J Biol Chem. , 2001) comment 8th(Q9AJU0) 59%, 3e-76 Streptomyces olivaceus_elmMIII O-methyltransferase III(Rhamnose C4'-methyltransferase) Elloramycin biosynthesisにおいて、L-rhamnose permethylationに応答する3つ(elmMI, elmMII, and elmMIII)のmethyltransferaseを同定した文献。elloramycin biosynthesis gene cluster中に存在するelmMI, elmMII, and elmMIIIについてクローニングし、それぞれの遺伝子をStreptomyces albusにおいてL-rhamnose生合成系遺伝子と共に発現させて、その生産物を調べている。その結果、3つのそれぞれ異なるelloramycin-type合成物の生産を確認している。また、3つのmethyltransferaseそれぞれを単独で発現させて、in vitroでのmethylating activityを確認している。

Sequence

>putative NDP-hexose O-methyltransferase [putative O-methyltransferase in D-chalcose pathway]
MKNVVSNTIYGDHPTFPVGMNVLDKELADRMRPGAEGPDVERGTFDADHRAEGLDVPTVA
HTMIGLQRLDNIQRCVERVLQDDVPGDLIETGVWRGGACILMRALLKAHGVEDRTVWLAD
SFAGVPVTSEDSHPLDRAMEFHHLNWVLSCSEEQVRENFARYGLLDEQVRFLPGMFADTL
PTAPIDRLAVLRVDGDLYESTRDALVNLYPKLSVGGFVIVDDYLIPACKQAVHDYRSEHG
IDEPIETVDVTGVYWRREH

>putative NDP-hexose O-methyltransferase [putative O-methyltransferase in D-chalcose pathway]
ATGAAGAACGTCGTGTCGAACACCATCTACGGGGATCACCCGACCTTCCCGGTCGGGATG
AACGTCCTGGACAAGGAACTGGCGGACCGCATGCGTCCGGGGGCGGAGGGCCCGGACGTC
GAGCGCGGGACCTTCGACGCCGACCACCGGGCCGAAGGACTGGACGTGCCGACGGTCGCG
CACACCATGATCGGCCTGCAACGCCTGGACAACATCCAGAGGTGCGTGGAGAGGGTCCTC
CAGGACGATGTTCCCGGTGATCTCATCGAGACCGGCGTGTGGCGCGGCGGCGCCTGCATC
CTGATGCGCGCGCTCCTCAAGGCGCACGGTGTGGAGGACCGCACCGTGTGGCTCGCCGAC
TCGTTCGCCGGTGTCCCGGTGACGTCCGAGGACAGCCACCCGCTCGACCGGGCGATGGAG
TTCCACCACCTGAACTGGGTACTGAGCTGTTCCGAGGAGCAGGTGAGGGAGAACTTCGCC
CGCTACGGCCTGCTGGACGAGCAGGTCAGGTTCCTGCCCGGCATGTTCGCCGACACGCTG
CCGACCGCGCCGATCGACCGGCTCGCGGTGCTGCGGGTGGACGGCGACCTGTACGAGTCG
ACGAGGGACGCGCTGGTCAACCTCTACCCCAAGCTGTCCGTGGGCGGGTTCGTGATCGTG
GACGACTACCTGATCCCGGCGTGCAAGCAGGCCGTCCACGACTACCGGAGCGAACACGGC
ATCGACGAGCCGATCGAGACGGTGGACGTGACGGGCGTCTACTGGCGCCGCGAGCACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR008884 Macrocin-O-methyltransferase (Family) PF05711 TylF
SignalP	No significant hit
TMHMM	No significant hit

Chalc_00040 Chalc_00060