Chalc_00060 : CDS information

Location

Organism	Streptomyces bikiniensis
Strain	NRRL 2737
Entry name	Chalcomycin
Contig	AY509120
Start / Stop / Direction	7,696 / 6,239 / - [in whole cluster] 7,696 / 6,239 / - [in contig]
Location	complement(6239..7696) [in whole cluster] complement(6239..7696) [in contig]
Type	CDS
Length	1,458 bp (485 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative SAM-dependent NDP-hexose C-4 deaminase
Product (GenBank)	putative D-chalcose pathway component
Gene
Gene (GenBank)	chmCV
EC number
Keyword	D-chalcose
Note
Note (GenBank)	predicted to participate with ChmCIV in production of 4-deoxy in D-chalcose pathway; similar to EryCV, NbmJ and DesII
Reference	ACC Q5SF97 PmId [15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004) comment Chalcomycin biosynthesis gene clusterに関する文献。 chmCV(485a.a.): D-chalcose pathway chmCV 自体の機能実験は行われておらず、erythromycinやpikromycin生合成経路におけるeryCVやdesIIとのホモログであることから、chmCIVと共にNDP-chalcose生産のためのC-4におけるdeoxygenationへの関与が予測されている。詳細な機能は不明の模様。
Related Reference	ACC Q9ZGH1 NITE Pikro_00150 PmId [19746907] Characterization and mechanistic studies of DesII: a radical S-adenosyl-L-methionine enzyme involved in the biosynthesis of TDP-D-desosamine. (J Am Chem Soc. , 2009) [20121093] Stoichiometry of the redox neutral deamination and oxidative dehydrogenation reactions catalyzed by the radical SAM enzyme DesII. (J Am Chem Soc. , 2010) [23329328] EPR-kinetic isotope effect study of the mechanism of radical-mediated dehydrogenation of an alcohol by the radical SAM enzyme DesII. (Proc Natl Acad Sci U S A. , 2013) comment 5th(Q9ZGH1) 76%, 0.0 Streptomyces venezuelae_desII [DoBISCUIT]SAM-dependent NDP-hexose C-4 deaminase/C-3 dehydrogenase [PMID: 19746907](2009) desosamine生合成において、DesIIはdTDP-4-amino-4,6-dideoxy-D-glucose → dTDP-3-keto-4,6-dideoxy-D-glucose への変換を担う。 DesIIはradical S-adenosyl-L-methionine(SAM) enzyme superfamilyのメンバーである。いくつかのhexoseのC-3, C-4での反応を触媒でき、C-4でのamino基はredox neutral deamination reactionを、C-4でのhydroxyl基の存在はC-3でのdehydrogenationを導く。C-4 hydroxyl基がaxialだと基質にならない。 DesII反応産物のHPLC解析、DesII reactionのsteady state kinetic parameters測定あり。 ↓この続報あり [PMID: 20121093](2010) [PMID: 23329328](2013)

Sequence

>putative SAM-dependent NDP-hexose C-4 deaminase [putative D-chalcose pathway component]
MTSHASTAADPVALCARPGADLPAAVHTVHRALVSDGRVGVDEGPTVARRLVRLAERYGN
HPFTPLEEARRMLDVDRASFGRLLGLFGRVPELRHAVENGPAGKYWQNTLLPLERRGVFD
AALAHKPAFPYVVGLYPGPSCMFRCHFCVRVTGARYDPSALESGNAMFASVIDEIPAGNP
YAMYFSGGLEPLTNPGLGALSSRAAARGLRPTVYTNSFALTERTLDRQPGVWDLHAVRTS
LYGLNDEEYEETTGKRAAFGRVRANLRRFQQLRSERESPIRLGLSYIVLPGRVHRLLDLV
DFIADLNEAAPDRPVDFLNVREDYSGREDGRLFEAERAELQEGLLAFEEAVSRRTPTLNI
DYGYALHSLKTGADAELLRIRPGTMRRSAHPQVSVQVDLLGDVYLYREAGFPDLTGADRY
VAGRVGPGTSLTEVVERFVDDGRLIAPHAGDEYFMDGFDQVVAARLNQLEADVVAGWEDA
RGFLR

>putative SAM-dependent NDP-hexose C-4 deaminase [putative D-chalcose pathway component]
ATGACCTCGCACGCCTCCACCGCGGCCGATCCGGTCGCCCTGTGCGCCCGCCCCGGCGCC
GACCTGCCGGCAGCGGTCCACACGGTGCACCGCGCCCTGGTCTCCGACGGCCGCGTCGGT
GTCGACGAGGGCCCGACGGTCGCGCGCCGGCTCGTGCGGCTGGCCGAGCGCTACGGGAAC
CACCCCTTCACGCCCCTGGAGGAGGCACGCCGGATGCTGGACGTGGACCGGGCCTCCTTC
GGCCGGCTCCTCGGCCTCTTCGGCCGTGTCCCGGAGCTGCGCCACGCCGTGGAGAACGGG
CCGGCGGGCAAGTACTGGCAGAACACCCTGCTGCCGCTGGAGCGACGGGGGGTCTTCGAC
GCGGCGCTCGCGCACAAGCCGGCCTTCCCCTATGTCGTGGGCCTGTACCCGGGGCCGAGC
TGCATGTTCCGCTGCCACTTCTGCGTCCGGGTGACCGGTGCGCGGTACGACCCGTCGGCG
CTGGAGTCCGGCAACGCGATGTTCGCGTCCGTCATCGACGAGATCCCGGCCGGCAACCCG
TACGCCATGTACTTCTCGGGCGGTCTGGAGCCCCTCACCAACCCGGGCCTGGGCGCGCTC
AGCAGCCGTGCGGCGGCTCGCGGGCTGCGGCCGACCGTGTACACGAACTCGTTCGCGCTC
ACCGAGCGGACGCTGGACAGGCAGCCCGGGGTGTGGGACCTGCACGCGGTGCGCACCTCG
CTGTACGGCTTGAACGACGAGGAGTACGAGGAGACCACGGGCAAGCGCGCCGCCTTCGGA
AGGGTCCGCGCCAACCTGCGGCGCTTCCAGCAGCTGCGGTCCGAGCGGGAGAGCCCGATC
CGGCTCGGGCTCAGCTACATCGTGCTGCCCGGGCGCGTCCACCGGCTGCTCGACCTCGTC
GACTTCATCGCCGACCTGAACGAGGCCGCGCCGGACCGGCCGGTGGACTTCCTGAACGTG
CGCGAGGACTACAGCGGCCGGGAGGACGGCAGGCTGTTCGAGGCCGAACGCGCCGAACTC
CAGGAGGGGCTGCTCGCGTTCGAGGAGGCGGTGAGCCGGCGGACCCCCACGCTGAACATC
GACTACGGCTACGCGTTGCACAGCCTGAAGACCGGCGCGGACGCCGAGCTGCTCAGGATC
AGGCCCGGAACCATGCGCCGGTCCGCGCATCCCCAGGTGTCCGTACAGGTGGACCTGCTG
GGTGACGTGTACCTGTACCGGGAGGCGGGGTTCCCGGATCTCACCGGAGCGGACCGGTAC
GTCGCGGGCCGGGTGGGGCCGGGCACCTCCCTGACGGAGGTCGTCGAGCGGTTCGTGGAC
GACGGCCGTCTGATAGCGCCGCACGCCGGTGACGAGTACTTCATGGACGGGTTCGACCAG
GTGGTGGCGGCGCGGCTCAACCAGCTGGAGGCCGATGTCGTGGCCGGCTGGGAGGACGCG
CGCGGCTTCCTGAGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR016863 Reductase, EryCV, predicted (Family) PIRSF027982 Reductase_EryCV_prd
SignalP	No significant hit
TMHMM	No significant hit

Chalc_00050 Chalc_00070