Chalc_00060 Chalc_00060   Chalc_00080 Chalc_00080

Chalc_00070 : CDS information

close this sectionLocation

Organism
StrainNRRL 2737
Entry nameChalcomycin
Contig
Start / Stop / Direction10,271 / 7,761 / - [in whole cluster]
10,271 / 7,761 / - [in contig]
Locationcomplement(7761..10271) [in whole cluster]
complement(7761..10271) [in contig]
TypeCDS
Length2,511 bp (836 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.4 resistance
Productputative beta-glucosidase
Product (GenBank)putative beta-glucosidase
Gene
Gene (GenBank)chmR
EC number3.2.1.21
Keyword
  • extracellular reactivation
Note
Note (GenBank)
  • possible extracellular reactivator of chalcomycin; similar to EryBI, OleR, DesR, NbmF, ORF30 lankamycin cluster
Reference
ACC
PmId
[15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004)
comment
Chalcomycin biosynthesis gene clusterに関する文献。

chmR(836a.a.): beta-glucosidase, extracellular reactivator of chalcomycin

chmR 自体の機能実験は行われておらず、oleandomycin biosynthetic gene clusterのoleRとの相同性より、機能を推定している。
Related Reference
ACC
Q9ZGH5
NITE
Pikro_00110
PmId
[9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998)
[14674753] Beta-glucosylation as a part of self-resistance mechanism in methymycin/pikromycin producing strain Streptomyces venezuelae. (Biochemistry. , 2003)
comment
6th(Q9ZGH5) 59%, 0.0
Streptomyces venezuelae_desR
[Pikro_00110]beta-glucosidase

--
[PMID: 9770448](1998)
12員環骨格を持つMethymycin and Neomethymycinと、14員環骨格を持つNarbomycin and Pikromycinの生合成で共有されるclusterの同定。

DesR: beta-Glucosidase (involved in resistance mechanism)

細菌の自己防衛のためのdrug inactivation-reactivation cycleに関連することが示されている。
Ref23←titleのみ, pubmedにない。
Biosynthesis of Desosamine: Molecular Evidence Suggesting beta-Glucosylation as a Self-Resistance Mechanism in Methymycin/Neomethymycin Producing Strain, Streptomyces venezuelae. J. Am. Chem. Soc./1998

--
[PMID: 14674753](2003)abstract
desRの破壊により、生物学的に不活性なglucosylated methymycin/neomethymycin productsが蓄積。
S. venezuelaeによって産生されるglucosylated methymycin/neomethymycinの加水分解できるbeta-glucosidaseとして、精製したDesRの触媒機能を確認。

desRは分泌タンパクに特徴的な配列を持つので、細胞外に輸送され、そこでglucosylated productsを活性化するために加水分解すると考えられる。

ただし、主要な自己抵抗性システムはPikR1 and PikR2による23S rRNAの修飾であり、このglycosylation/deglycosylationはS. venezuelaeにおいて二次的な自己防衛メカニズムである。
ACC
O68843
PmId
[9680207] Two glycosyltransferases and a glycosidase are involved in oleandomycin modification during its biosynthesis by Streptomyces antibioticus. (Mol Microbiol. , 1998)
comment
10th(O68843) 57%, 0.0
Streptomyces antibioticus_oleR
Glycosidase OleR

oleRをStreptomyces lividansで発現させて、OleRが培養液に分泌されていることを示している。また、glycosidase activityを確認し、glycosylated oleandomycinからactive型のoleandomycinへと変換することを示している。

close this sectionSequence

selected fasta
>putative beta-glucosidase [putative beta-glucosidase]
MRTATSAERTSVSMLFGTTRTGRRIRRTVGSALAALCVGGLLTAPSAAGAPAAEPGTARV
RGLVAKMTLDEKISFVHWTTGPVGGPTMTGIGYLPGVPRLGIPELRTADGPVGIRLLGGT
ATAMPTPVALAATFDERLAEEYGTVLGREGRALGQDIVLGPMTNVIRVPHAGRNFETYSE
DPLLSSRMAAHEVRGIQNQGLMATVKHFAANNQEYQRETIDAVVDEQTLQEVELPAFRSA
VRAGAASVMCSYNKVNGAHACGNEHLLQEVLREQWDFRGWVVSDWLATHATGDITRGLDQ
ELGVELTLGQPVPESKYFSSALRAAVRDGSVPEATLDRSVVRILGQMERFGLLDGKATER
PQRDPEAGRAAARTIAENGGVLLRNERRTLPLTGEDATDIAVIGNSAKHPKVTGNGSAHV
IPDRATAPVDALARRAGEKARVVHEPGEDLVGVPVPESSLTPAFTSGKQLDPSGQGVFYE
GRLTVPADGDYKIAFTAVGGVANLQIAGQSAVLGTEAFGTVTTTMRLTRGTHAVTMNGWA
FEQTPLSVELSWVTPEAARDDFDRAVAAAAEARTAVVFAHDDSAEGVDRSSLSLPGRQDE
LIAAITKVNPRTIVVLNTGSSVLMPWLRETAAVLEMWYPGQEGAEATAALLFGDANPSGR
LTQTFPATETGHPMAGDPHRYPGVDGKETYSEGLDVGYRWYDRTGVAPLFPFGYGLSYTT
FAYSDLSVARTARGLEATVTVRNTGDRAGRETVQVYLGASPDTQAPQALRKLAGFTKVTL
RPGEQRRVTVPVDEQQLRYWDTAAGTWKPGTGRRAVHVGPSAAETSLTTSVTVPSR
selected fasta
>putative beta-glucosidase [putative beta-glucosidase]
ATGAGGACGGCAACCTCGGCAGAAAGGACGTCCGTCAGCATGCTGTTCGGCACGACGAGG
ACGGGTCGCCGCATCCGGCGCACCGTCGGATCCGCGCTCGCGGCCCTGTGCGTCGGCGGT
CTGCTCACCGCCCCCTCCGCCGCCGGTGCTCCGGCCGCCGAGCCCGGGACGGCGCGGGTG
CGCGGGCTGGTGGCGAAGATGACCCTCGACGAGAAGATCTCGTTCGTCCACTGGACCACC
GGGCCCGTGGGCGGGCCGACCATGACCGGCATCGGCTATCTGCCGGGCGTGCCCCGGCTC
GGCATCCCCGAGCTGCGCACCGCCGACGGCCCGGTCGGCATCCGTCTGCTCGGCGGTACC
GCCACCGCCATGCCCACCCCCGTGGCGCTCGCCGCCACCTTCGACGAACGCCTCGCCGAG
GAGTACGGCACCGTCCTGGGCCGGGAGGGACGGGCCCTCGGACAGGACATCGTCCTCGGT
CCCATGACCAACGTCATCCGGGTGCCGCACGCGGGGCGGAACTTCGAGACCTACAGCGAG
GATCCTCTGCTGTCGTCCCGGATGGCCGCCCACGAGGTCCGCGGCATCCAGAACCAGGGC
CTGATGGCCACGGTCAAGCACTTCGCCGCGAACAACCAGGAGTACCAGCGGGAGACCATC
GACGCCGTGGTCGACGAGCAGACGCTCCAGGAGGTGGAGCTGCCGGCCTTCCGCAGCGCC
GTGCGGGCGGGCGCCGCCTCGGTGATGTGCTCGTACAACAAGGTCAACGGCGCGCACGCA
TGCGGGAACGAGCACCTGTTGCAGGAGGTGCTGCGCGAACAGTGGGACTTCCGCGGCTGG
GTGGTGTCCGACTGGCTGGCGACCCACGCCACCGGCGACATCACCAGGGGCCTCGACCAG
GAACTGGGCGTCGAGCTGACCCTGGGGCAGCCGGTGCCGGAATCGAAGTACTTCTCCTCC
GCGCTCAGGGCCGCCGTGCGGGACGGCAGCGTCCCCGAGGCGACGCTCGATCGGTCCGTC
GTCCGCATCCTCGGCCAGATGGAGCGCTTCGGCCTGCTCGACGGCAAGGCGACCGAGCGC
CCGCAGCGTGATCCCGAGGCCGGCCGCGCGGCGGCCCGCACCATCGCCGAGAACGGCGGG
GTGCTGCTGCGCAACGAGCGCCGGACGCTGCCCCTGACCGGTGAGGACGCCACGGACATC
GCCGTCATCGGCAACAGCGCCAAGCACCCCAAGGTCACCGGCAACGGCAGCGCGCACGTC
ATACCCGACCGGGCGACCGCACCCGTCGACGCCCTCGCACGGCGCGCCGGCGAGAAGGCC
CGGGTCGTCCACGAGCCCGGTGAGGACCTGGTCGGCGTGCCCGTGCCCGAAAGCTCGCTC
ACCCCCGCCTTCACGAGCGGGAAGCAACTGGATCCCAGCGGCCAGGGCGTCTTCTACGAG
GGTCGGCTCACCGTCCCCGCCGACGGCGACTACAAGATCGCCTTCACCGCCGTCGGCGGG
GTCGCCAACCTGCAGATCGCGGGGCAGTCCGCTGTCCTCGGCACCGAGGCGTTCGGCACC
GTCACCACCACGATGCGGCTGACCCGCGGCACCCACGCGGTGACCATGAACGGCTGGGCC
TTCGAACAGACCCCGCTGTCGGTGGAGCTGAGCTGGGTCACTCCCGAGGCTGCCCGGGAC
GACTTCGACCGCGCCGTCGCGGCGGCGGCCGAGGCCCGCACCGCCGTCGTCTTCGCCCAC
GACGACAGTGCCGAGGGCGTGGACCGGTCCTCGCTGTCCCTGCCCGGCCGACAGGACGAG
CTGATCGCGGCGATCACCAAGGTCAACCCCCGCACGATCGTCGTCCTCAACACCGGTTCC
TCGGTGCTCATGCCCTGGCTGCGTGAGACGGCCGCCGTACTGGAGATGTGGTACCCGGGG
CAGGAGGGCGCGGAGGCCACCGCTGCCCTGCTGTTCGGCGACGCGAATCCCAGCGGCCGG
CTGACCCAGACCTTCCCGGCCACCGAGACCGGCCATCCCATGGCCGGTGACCCGCACCGC
TACCCGGGTGTCGACGGCAAGGAGACCTATTCCGAGGGACTGGATGTCGGCTACCGGTGG
TACGACCGGACCGGCGTGGCCCCGCTCTTCCCGTTCGGGTACGGCCTGTCGTACACCACC
TTCGCCTACAGCGACCTGTCCGTGGCGCGCACCGCCCGGGGGCTGGAGGCCACGGTCACC
GTCCGCAACACCGGTGACCGGGCGGGCAGGGAGACCGTCCAGGTCTACCTCGGGGCAAGT
CCGGACACCCAGGCCCCCCAGGCGCTGCGCAAACTCGCCGGATTCACCAAGGTCACCCTC
CGGCCGGGAGAACAGCGGCGGGTCACCGTCCCCGTCGACGAGCAGCAACTGCGCTACTGG
GACACCGCCGCCGGCACGTGGAAGCCGGGCACCGGCAGGAGGGCCGTACACGTGGGCCCG
TCCGCGGCCGAGACGTCCCTGACCACATCCGTCACGGTGCCGTCCCGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001764 Glycoside hydrolase, family 3, N-terminal (Domain)
[99-115] 2.19999900980708e-32 PR00133 [124-143] 2.19999900980708e-32 PR00133 [170-186] 2.19999900980708e-32 PR00133 [201-217] 2.19999900980708e-32 PR00133 [270-288] 2.19999900980708e-32 PR00133
PR00133   GLHYDRLASE3
[55-359] 9.60000000000003e-104 G3DSA:3.20.20.300
G3DSA:3.20.20.300   Glyco_hydro_3_N
[68-344] 9.80000000000012e-86 PF00933
PF00933   Glyco_hydro_3
IPR002772 Glycoside hydrolase family 3 C-terminal domain (Domain)
[380-721] 4.30000170645869e-50 SSF52279
SSF52279   Glyco_hydro_3_C
[367-449] 4.70000000000003e-62 G3DSA:3.40.50.1700 [564-721] 4.70000000000003e-62 G3DSA:3.40.50.1700
G3DSA:3.40.50.1700   G3DSA:3.40.50.1700
[381-698] 3.40000000000004e-59 PF01915
PF01915   Glyco_hydro_3_C
IPR017853 Glycoside hydrolase, superfamily (Domain)
[45-379] 2.7000136937899e-93 SSF51445
SSF51445   Glyco_hydro_cat
SignalP
[1-52] 1 Signal
Bacteria, Gram-positive   
[1-49] 1 Signal
Bacteria, Gram-negative   
[1-49] 0.973 Signal
Eukaryota   
TMHMM
[29-51] Transmembrane (i-o)
Transmembrane 1   
Chalc_00060 Chalc_00060   Chalc_00080 Chalc_00080
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