Chalc_00080 : CDS information

Location

Organism	Streptomyces bikiniensis
Strain	NRRL 2737
Entry name	Chalcomycin
Contig	AY509120
Start / Stop / Direction	11,511 / 10,306 / - [in whole cluster] 11,511 / 10,306 / - [in contig]
Location	complement(10306..11511) [in whole cluster] complement(10306..11511) [in contig]
Type	CDS
Length	1,206 bp (401 aa)

Annotation

Category	3.3 modification reduction
Product	putative epoxidase putative cytochrome P450
Product (GenBank)	P450
Gene
Gene (GenBank)	chmPII
EC number
Keyword
Note
Note (GenBank)	putative macrolide C12,C13-epoxidase; similar to MycG, LnmZ
Reference	ACC Q5SFD6 PmId [15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004) comment Chalcomycin biosynthesis gene clusterに関する文献。 chmPII(401a.a.): P450; 12,13-epoxidase chmPII自体の機能実験は行われていないが、chalcomycin生産の最終段階の酸化反応（12,13-epoxidasion)にchmPI(C-8 hydroxylation)と共に関与しているとされている。
Related Reference	ACC Q331R6 PmId [21069297] Identification and characterization of gerPI and gerPII involved in epoxidation and hydroxylation of dihydrochalcolactone in Streptomyces species KCTC 0041BP. (Arch Microbiol. , 2011) comment 3rd(Q331R6) 91%, 0.0 Streptomyces sp. KCTC 0041BP_gerPI Cytochrome P-450 dihydrochalcomycin生合成clusterにある3つのCYP450のうち、gerPI, PII の特徴づけ。 gerPI の破壊は主に12,13-de-epoxydihydrochalcomycinを蓄積した。 gerPII の破壊は8-dehydroxy-12,13-de-epoxydihydrochalcomycinを蓄積した。よって、GerPII がC-8でのhydroxylationを、続いてGerPI がC(12)-C(13) positionでのepoxidationを触媒する。

Sequence

>putative epoxidase [P450]
MGLPLTSTKTAPVSYPFGRPEGLDLDEAYEQARKSEGLLWVHMPYGEPGWLVSRYDDARF
VLGDRRFSHAAEAENDAPRMRELRTPNGIIGMDAPDHTRLRGLVTKAFTPRRVEAMRPHV
RRMTASLLRDMTALGSPVDLVDHYAVPLPVAVICGLLGVPEEDRDLFRGWCEIAMSTSSL
TAEDHVRLAGELTGYLADLITARRAAPRDDLVSALVEARDAQGRLSQEELVDLIVFLLFA
GHETTASQISNFVLVLLEQPDQLALLRDRPDLLDNAVEELTRFVPLGSQAGFPRYATEDV
EVGGTLVRAGDPVLVQMNAANRDALRFRSPGVLDITRDDAGRHLGYGHGPHHCLGASLAR
LELQEALRTLLDELPGLHLAQPVEWKTEMVVRGPRTMLVGW

>putative epoxidase [P450]
ATGGGGCTCCCCTTGACCAGCACGAAGACCGCACCCGTCTCCTATCCCTTCGGCCGCCCG
GAGGGACTGGACCTGGACGAGGCGTACGAGCAGGCGCGGAAGAGCGAAGGACTCCTCTGG
GTCCACATGCCGTACGGGGAGCCGGGCTGGCTCGTCTCCCGTTACGACGACGCGCGTTTC
GTCCTCGGCGACCGGCGGTTCAGCCACGCCGCGGAGGCGGAGAACGACGCGCCGCGCATG
CGGGAGCTGCGGACGCCCAACGGCATCATCGGCATGGACGCGCCGGACCACACCAGGCTG
CGCGGGCTGGTCACCAAGGCGTTCACCCCGCGCCGCGTGGAGGCCATGCGGCCGCACGTC
CGAAGGATGACGGCGTCGCTGCTCCGGGACATGACGGCGCTCGGATCGCCCGTCGACCTC
GTGGACCACTACGCCGTCCCCCTTCCCGTGGCGGTCATCTGCGGCCTGCTGGGCGTGCCC
GAGGAGGACCGTGACCTGTTCCGCGGCTGGTGCGAGATAGCGATGTCCACCAGCTCGCTC
ACCGCCGAGGACCATGTGCGGCTGGCCGGCGAGCTGACCGGCTATCTGGCGGACCTGATC
ACGGCCCGCCGCGCCGCGCCGCGAGACGATCTCGTCTCGGCGCTCGTCGAGGCCCGTGAC
GCCCAGGGCCGGCTGTCGCAGGAGGAGTTGGTCGACCTGATCGTCTTCCTCCTGTTCGCC
GGGCACGAGACCACGGCCAGCCAGATCAGCAACTTCGTCCTGGTCCTGCTCGAACAGCCG
GACCAGCTGGCCCTCCTCCGGGACCGTCCTGACCTGCTGGACAACGCGGTGGAAGAGCTG
ACGCGGTTCGTTCCGCTCGGCAGCCAGGCGGGATTCCCGCGCTACGCCACCGAGGACGTC
GAGGTGGGCGGGACGCTGGTGCGGGCGGGGGATCCGGTGCTGGTCCAGATGAACGCCGCC
AACCGGGACGCCCTGCGCTTCCGCTCGCCCGGCGTGCTCGACATCACCCGCGACGACGCC
GGCCGGCACCTGGGGTACGGACACGGTCCGCATCACTGCCTCGGCGCGTCCCTCGCGCGG
CTGGAGCTCCAGGAGGCGCTGCGGACCCTGCTCGACGAACTCCCCGGGCTGCACCTCGCC
CAGCCCGTCGAGTGGAAGACGGAGATGGTGGTGCGGGGCCCGCGCACGATGCTCGTGGGC
TGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PR00385 P450 G3DSA:1.10.630.10 Cyt_P450 SSF48264 Cytochrome_P450 PF00067 p450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450
SignalP	No significant hit
TMHMM	No significant hit

Chalc_00070 Chalc_00090