Chalc_00090 : CDS information

Location

Organism	Streptomyces bikiniensis
Strain	NRRL 2737
Entry name	Chalcomycin
Contig	AY509120
Start / Stop / Direction	12,772 / 11,549 / - [in whole cluster] 12,772 / 11,549 / - [in contig]
Location	complement(11549..12772) [in whole cluster] complement(11549..12772) [in contig]
Type	CDS
Length	1,224 bp (407 aa)

Annotation

Category	3.3 modification reduction
Product	putative cytochrome P450
Product (GenBank)	P450
Gene
Gene (GenBank)	chmPI
EC number
Keyword	C-8 hydroxylate
Note
Note (GenBank)	putative macrolide C8-hydroxylase; similar to OleP
Reference	ACC Q5SFD5 PmId [15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004) comment Chalcomycin biosynthesis gene clusterに関する文献。 chmPI(407a.a.): P450; C-8 hydroxylase chmPI自体の機能実験は行われていないが、chalcomycin生産の最終段階において、C-8 hydroxylationに関与しているとされている。
Related Reference	ACC Q331R5 PmId [21069297] Identification and characterization of gerPI and gerPII involved in epoxidation and hydroxylation of dihydrochalcolactone in Streptomyces species KCTC 0041BP. (Arch Microbiol. , 2011) comment 3rd(Q331R5) 93%, 0.0 Streptomyces sp. KCTC 0041BP_gerPII Cytochrome P-450 dihydrochalcomycin生合成clusterにある3つのCYP450のうち、gerPI, PII の特徴づけ。 gerPI の破壊は主に12,13-de-epoxydihydrochalcomycinを蓄積した。 gerPII の破壊は8-dehydroxy-12,13-de-epoxydihydrochalcomycinを蓄積した。よって、GerPII がC-8でのhydroxylationを、続いてGerPI がC(12)-C(13) positionでのepoxidationを触媒する。

Sequence

>putative cytochrome P450 [P450]
MTSSCPVHRTRPYPFSPPEGIALDPLYSRLREHEPVSRIRMPYGGEAWLLTRHADVRAVL
GDPRFSMEAGAGRDVPRPTEYPLPAGGLISMDPPGHTRLRRLAGKAFTARRVEELRPRVS
RFTDELLDGMVARDEPSGEVMEDLALPVSISVICGLLGVSYDDRHLFRDFSEALVSSSLG
PAEVQRATEDFSAYMADLVADRRARPTDDFLSTMVQARDEGDRLSEAELLMMGSGLLISG
YETTATQIGNFVLLLLDDRRLYERLVTEPDLVPSAVEELLRFTPLSTLDGFARIALEDVE
IGGTLIRAGEAVITSIASANLDDTAFPGAASLDLARAQNPHLGFGHGAHYCMGAPLARLE
LQVVLSTLVERLPELRLSVPASELRWRAGSLLRTPEAVPVTWGGSAA

>putative cytochrome P450 [P450]
ATGACGTCTAGCTGTCCGGTCCACCGGACTCGCCCGTACCCCTTCTCCCCGCCCGAGGGC
ATCGCGCTGGATCCGCTCTACAGCCGTCTGCGGGAGCACGAGCCCGTGTCCCGGATCCGC
ATGCCGTACGGCGGTGAGGCCTGGCTGCTGACCCGACACGCCGACGTGAGGGCGGTCCTG
GGAGACCCGCGGTTCAGCATGGAGGCCGGGGCCGGACGTGACGTGCCCCGGCCCACCGAG
TACCCGCTGCCCGCCGGCGGTCTCATCTCCATGGACCCGCCCGGACACACCAGGCTCCGG
CGGCTCGCCGGGAAGGCGTTCACCGCCCGCCGGGTGGAGGAACTGCGCCCGCGGGTCTCA
CGGTTCACCGATGAACTGCTCGACGGCATGGTCGCGCGCGACGAGCCGTCCGGCGAGGTC
ATGGAAGACCTGGCGCTGCCCGTGTCGATCTCCGTGATCTGCGGACTGCTGGGCGTCTCC
TACGACGACCGCCACCTCTTCCGGGACTTCTCCGAGGCGCTGGTGTCCTCCTCCCTGGGG
CCCGCCGAAGTGCAGCGGGCGACCGAGGACTTCTCCGCCTACATGGCCGACCTCGTGGCC
GATCGCCGCGCCCGGCCCACCGACGACTTCCTCAGCACCATGGTCCAGGCCCGTGACGAA
GGCGACCGGCTCTCCGAGGCCGAGCTCCTCATGATGGGCTCCGGCCTGCTGATCAGCGGA
TACGAGACGACCGCCACCCAGATCGGCAATTTCGTCCTCCTCCTGCTCGACGACCGCCGC
CTCTACGAGCGCCTGGTGACCGAACCTGACCTGGTTCCCTCCGCCGTCGAGGAGCTGCTG
CGCTTCACCCCGCTGAGCACCCTGGACGGGTTCGCCCGCATCGCCTTGGAGGACGTCGAG
ATCGGCGGCACGCTCATCCGCGCGGGCGAGGCGGTCATCACCTCGATCGCGTCCGCGAAC
CTCGACGACACGGCGTTTCCCGGTGCCGCCTCCCTCGACCTGGCCCGTGCCCAGAACCCG
CACCTCGGCTTCGGTCACGGCGCCCACTACTGCATGGGCGCCCCCCTGGCCCGGCTCGAA
CTGCAGGTCGTGCTGAGCACCCTGGTCGAGCGCCTGCCGGAGCTCCGGCTGTCCGTCCCC
GCGAGTGAGCTTCGCTGGCGGGCCGGCTCCCTCCTGCGCACCCCCGAGGCGGTCCCCGTC
ACCTGGGGCGGCTCGGCCGCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PR00385 P450 PF00067 p450 G3DSA:1.10.630.10 Cyt_P450 SSF48264 Cytochrome_P450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	No significant hit
TMHMM	No significant hit

Chalc_00080 Chalc_00100