Chalc_00140 Chalc_00140   Chalc_00160 Chalc_00160

Chalc_00150 : CDS information

close this sectionLocation

Organism
StrainNRRL 2737
Entry nameChalcomycin
Contig
Start / Stop / Direction17,551 / 18,813 / + [in whole cluster]
17,551 / 18,813 / + [in contig]
Location17551..18813 [in whole cluster]
17551..18813 [in contig]
TypeCDS
Length1,263 bp (420 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productcytochrome P450
Product (GenBank)P450
Gene
Gene (GenBank)chmHI
EC number
Keyword
  • C-20 hydroxylate
Note
Note (GenBank)
  • putative macrolide C20-hydroxylase; similar to TylF
Reference
ACC
PmId
[15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004)
[15489173] Production of hybrid 16-membered macrolides by expressing combinations of polyketide synthase genes in engineered Streptomyces fradiae hosts. (Chem Biol. , 2004)
comment
[PMID:15561847](2004)
Chalcomycin biosynthesis gene clusterに関する文献。

chmHI(420a.a.): P450; C20 hydroxylase

chmHI 自体の機能実験は行われていないが、TylHIとの相同性よりP450として、chmHIIにエンコードされているferredoxinと共にmacrolactoneのC-20のhydroxylationに関与するとしている。

--
[PMID:15489173](2004)
cluster論文と同じ著者ら。

内容から判断してchmH=chmHI
chmHに関する引用論文は上記cluster論文。

tylosin PKSは不活化されているがPKS後修飾genesは保存のあるS.fradiae由来株で、chalcomycinとspiramycinのPKS hybridを発現して得た化合物はC-4にmethoxy基あり。これがあると、C-14のmethyl基をhydroxylationするTylHの働きが非効率。しかし、TylH homologであるChmHは効率的なhydroxylationをもたらすことができる。
Related Reference
ACC
B3XWK3
PmId
[18640605] Increase in pladienolide D production rate using a Streptomyces strain overexpressing a cytochrome P450 gene. (J Biosci Bioeng. , 2008)
comment
<Cytochrome P450 monooxygenase (psmA), Streptomyces sp. A-1544, BLAST 12; id=48%>

Streptomyces sp. A-1544にpladienolide B 16-hydroxylaseをエンコードするpsmAを過剰発現させると、pladienolide B より pladienolide Dへの変換が15倍高くなることを示している。また、この変換にはpsmAの下流のferredoxinをエンコードするpsmBの産物が必要であることが示されている。

close this sectionSequence

selected fasta
>cytochrome P450 [P450]
MLDVDHLLPIAFRVRKSMKSSKVVHSRPAEAGVAWPVARTCPFTLPDQYAEKRKNEPICR
AQVWDDSRTWLITKHEHVRALLADPRVTVDPAKLPRLSPSDGDGGGFRSLLTMDPPDHNA
LRRMLISEFSVHRVREMRPGIERTVHGLLDGILERRGPVDLVAELALPMSTLVICQLLGV
PYEDREFFQERSEQATRVGGSQESLTALLELRDYLDRLVTAKIETPGDDLLCRLIASRLH
TGEMRHAEIVDNAVLLLAAGHETSAAMVALGILTLLRHPGALAELRGDGTLMPQTVDELL
RFHSIADGLRRAVTEDIELGGITLRAGDGLIVSLASANRDESAFASPDGFDPHHPASRHV
AFGYGPHQCLGQNLARLELEVTLGAVVERIPTLRLAGDADALRVKQDSTIFGLHELPVEW
selected fasta
>cytochrome P450 [P450]
ATGTTGGACGTAGATCACCTTCTCCCGATTGCATTCAGGGTGAGGAAATCCATGAAATCT
TCAAAAGTCGTTCACAGCCGTCCTGCGGAAGCGGGCGTCGCATGGCCCGTCGCGCGAACC
TGCCCCTTTACGCTCCCTGATCAGTACGCAGAGAAGCGCAAGAACGAGCCCATATGCCGG
GCTCAGGTCTGGGACGACTCCAGAACCTGGCTCATCACCAAGCACGAGCACGTACGAGCC
CTTCTCGCCGACCCCCGGGTCACCGTCGACCCGGCCAAGCTGCCCAGGCTCTCCCCCTCC
GACGGCGACGGCGGCGGCTTCCGGTCCCTGCTGACCATGGACCCCCCGGACCACAACGCC
CTCCGCCGCATGCTCATATCCGAGTTCAGCGTGCACCGGGTCCGGGAGATGCGCCCGGGC
ATCGAGCGCACCGTGCACGGGCTGCTGGACGGGATCCTCGAACGGCGGGGGCCGGTGGAC
CTGGTGGCCGAACTCGCGCTGCCGATGTCCACCCTGGTGATCTGTCAGCTCCTCGGAGTG
CCCTACGAAGACCGCGAGTTCTTCCAGGAACGCAGCGAACAGGCCACCCGGGTGGGCGGG
AGCCAGGAATCGCTGACCGCGCTCCTGGAACTACGGGACTACCTGGACCGGTTGGTCACC
GCGAAGATCGAGACGCCGGGTGACGACCTGCTGTGCCGGCTCATCGCCAGTCGACTGCAC
ACCGGTGAGATGCGACACGCCGAGATCGTGGACAACGCCGTGCTGCTGCTCGCCGCCGGC
CACGAGACCAGTGCCGCCATGGTGGCACTGGGCATCCTGACACTGTTGCGGCACCCCGGC
GCCCTGGCGGAGTTGCGGGGCGACGGTACGCTGATGCCGCAGACGGTCGACGAACTCCTG
CGTTTCCACTCCATCGCGGACGGCCTTCGACGGGCGGTCACGGAGGACATCGAACTCGGC
GGCATCACGCTGCGCGCCGGAGACGGCCTCATCGTCTCGCTGGCCTCCGCCAACCGCGAC
GAGAGCGCCTTCGCCTCCCCGGACGGCTTCGACCCGCACCATCCGGCGAGCCGGCACGTC
GCCTTCGGCTACGGCCCCCACCAGTGCCTGGGCCAGAACCTGGCCCGGCTGGAGCTGGAG
GTCACCCTGGGCGCGGTGGTGGAGCGCATTCCCACGCTCAGGCTGGCCGGCGACGCCGAC
GCACTGCGCGTCAAACAGGATTCGACCATCTTCGGGCTGCACGAGCTGCCGGTCGAGTGG
TGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[259-276] 2.59999946718995e-08 PR00385 [294-305] 2.59999946718995e-08 PR00385 [360-369] 2.59999946718995e-08 PR00385 [369-380] 2.59999946718995e-08 PR00385
PR00385   P450
[229-389] 3.09999999999998e-29 PF00067
PF00067   p450
[18-420] 7.40000780398351e-96 SSF48264
SSF48264   Cytochrome_P450
[39-420] 2.99999999999998e-104 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
IPR002397 Cytochrome P450, B-class (Family)
[113-124] 7.30000590915205e-54 PR00359 [160-176] 7.30000590915205e-54 PR00359 [177-192] 7.30000590915205e-54 PR00359 [213-235] 7.30000590915205e-54 PR00359 [294-305] 7.30000590915205e-54 PR00359 [311-338] 7.30000590915205e-54 PR00359 [339-354] 7.30000590915205e-54 PR00359 [360-369] 7.30000590915205e-54 PR00359 [369-380] 7.30000590915205e-54 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[362-371] PS00086
PS00086   CYTOCHROME_P450
SignalP
[1-31] 0.433 Signal
Bacteria, Gram-positive   
[1-31] 0.254 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Chalc_00140 Chalc_00140   Chalc_00160 Chalc_00160
Page top