Chalc_00130 Chalc_00130   Chalc_00150 Chalc_00150

Chalc_00140 : CDS information

close this sectionLocation

Organism
StrainNRRL 2737
Entry nameChalcomycin
Contig
Start / Stop / Direction17,290 / 16,523 / - [in whole cluster]
17,290 / 16,523 / - [in contig]
Locationcomplement(16523..17290) [in whole cluster]
complement(16523..17290) [in contig]
TypeCDS
Length768 bp (255 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative hexose C-3'' O-methyltransferase
Product (GenBank)putative 3'OH-methyltransferase in D-mycinose pathway
GenechmMII
chmF
Gene (GenBank)chmMII
EC number2.1.1.-
Keyword
  • D-mycinose
Note
Note (GenBank)
  • member of O-methyltransferase homology family, including TylF
Reference
ACC
PmId
[15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004)
comment
Chalcomycin biosynthesis gene clusterに関する文献。

論文中ではchmMIIでは無く、chmFとなっている。
chmF(255a.a.): 3-O-methyltransferase; D-mycinose pathway

chmF 自体の機能実験は行われていないが、TylFとの相同性より3-O-methyltransferaseとして、同じくmethyltransferaseとして機能すると推定しているchmEと共に、5-O-chalcosyl-20-O-(6-deoxyallosyl)-macrolactoneより5-O-chalcosyl-20-O-mycinosyl-macrolactoneを生じると提唱している。
Related Reference
ACC
Q49492
PmId
[8163162] A gene encoding mycinamicin III O-methyltransferase from Micromonospora griseorubida. (Gene. , 1994)
[19415708] Functional analysis of MycE and MycF, two O-methyltransferases involved in the biosynthesis of mycinamicin macrolide antibiotics. (Chembiochem. , 2009)
[20158522] ( , )
[25692963] ( , )
comment
BLAST id68%
Micromonospora griseorubida_mycF
Mycinamicin III 3''-O-methyltransferase

--
[PMID: 8163162](1994)
macrocin O-methyltransferase (tylF gene product)と相同性の高い遺伝子をacZ promoteを付けてE. coliで発現させたところ、IPTG誘導下でのみmycinamicin III (MIII) O-methyltransferase (MOMT) activity を確認し、その遺伝子をmycFとしている。

--
[PMID: 19415708](2009)
mycinamicin生合成における2つのO-methyltransferase、MycE と MycFの機能を解析している。
mycE, mycFそれぞれの遺伝子をHis-tagを付けてE. coliで発現させて精製し、各々の機能を調べている。

各酵素はS-adenosyl-L-methionine (SAM)-dependent deoxysugar methyltransferaseであることが確認された。MycEの最適反応には、MycFよりも明白にMg(2+)が必要。

MycE → aglycone付着6-deoxyalloseのC2''-OHをmethylation = javose
MycF → aglycone付着javoseのC3''-OHをmethylation = mycinose
ACC
Q9ALM9
NITE
Spino_00140
PmId
[20158237] Biosynthesis of spinosyn in Saccharopolyspora spinosa: synthesis of permethylated rhamnose and characterization of the functions of SpnH, SpnI, and SpnK. (J Am Chem Soc. , 2010)
comment
BLAST id61%
Saccharopolyspora spinosa_spnH
[Spino_00140]rhamnose 4-O-methyltransferase

rhamnose methyltransferasesとされるSpnH, SpnI, and SpnKの機能検証、そのpermethylation過程の役割を確認。
SpnH, SpnI, and SpnK がそれぞれrhamnose 4'-, 2'-, and 3'-O-methyltransferaseであることが確立された。
また、SpnI→SpnK→SpnHという順番で働くこともわかっている。

close this sectionSequence

selected fasta
>putative hexose C-3'' O-methyltransferase [putative 3'OH-methyltransferase in D-mycinose pathway]
MTVIAAEADLYLDLLKKVVTNMIYEDQTNVAGLITSSSYSAELRSVGEDFPRVAHSMVGL
KRLDNLQKCLEDVLRDGVPGDFAETGVWRGGACIFARGVFRAHGVRDRKVWVADSFQGFP
KTTEDDHQLDVDIDLGQYNDVLSIPVDVETVKGNFARYGLLDDQVRFLPGWFKDTMPTAP
IERLAVLRLDGDSYAATREVLTNLYHKVSDGGYVIVDDYCIPACRQAVHEFRDEHGITDE
IHQIDRQGSYWRRSS
selected fasta
>putative hexose C-3'' O-methyltransferase [putative 3'OH-methyltransferase in D-mycinose pathway]
ATGACTGTCATAGCTGCCGAAGCGGACCTGTATCTCGACCTTCTGAAGAAGGTCGTGACG
AACATGATTTACGAGGACCAGACCAATGTCGCCGGCCTCATCACCAGCAGCTCGTATTCC
GCGGAACTGCGCTCGGTGGGTGAGGACTTTCCGCGCGTGGCGCACTCGATGGTCGGTCTC
AAGCGGCTCGACAACCTGCAGAAGTGCCTGGAGGACGTGCTGCGCGACGGCGTGCCGGGG
GACTTCGCGGAGACCGGGGTCTGGCGGGGCGGGGCCTGCATCTTCGCCCGCGGCGTCTTC
CGGGCGCACGGCGTGCGGGACCGGAAGGTGTGGGTCGCGGACTCCTTCCAGGGTTTCCCC
AAGACCACCGAGGACGACCACCAGCTGGACGTCGACATCGACCTCGGCCAGTACAACGAC
GTCCTCAGCATCCCGGTCGACGTGGAGACCGTGAAGGGGAACTTCGCGCGCTACGGACTC
CTGGACGACCAGGTCCGCTTCCTCCCGGGCTGGTTCAAGGACACGATGCCCACCGCGCCC
ATCGAGAGGCTCGCGGTGCTGAGGCTCGACGGGGACTCGTACGCAGCCACCAGGGAGGTT
CTCACCAACCTCTACCACAAGGTGTCGGACGGCGGGTACGTCATCGTCGACGACTACTGC
ATTCCGGCGTGCCGGCAGGCCGTCCACGAGTTCCGGGACGAGCACGGCATCACGGACGAG
ATCCACCAGATCGATCGCCAGGGTTCCTACTGGAGACGCTCCAGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR008884 Macrocin-O-methyltransferase (Family)
[6-254] 4.89999999999996e-128 PF05711
PF05711   TylF
SignalP No significant hit
TMHMM No significant hit
Chalc_00130 Chalc_00130   Chalc_00150 Chalc_00150
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