Chalc_00130 : CDS information

Location

Organism	Streptomyces bikiniensis
Strain	NRRL 2737
Entry name	Chalcomycin
Contig	AY509120
Start / Stop / Direction	16,459 / 15,869 / - [in whole cluster] 16,459 / 15,869 / - [in contig]
Location	complement(15869..16459) [in whole cluster] complement(15869..16459) [in contig]
Type	CDS
Length	591 bp (196 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	dTDP-4-keto-6-deoxy-D-glucose 3-epimerase
Product (GenBank)	putative 3-epimerase in D-allose pathway
Gene
Gene (GenBank)	chmJ
EC number	5.1.3.27
Keyword	6-deoxy-D-allose (D-mycinose)
Note
Note (GenBank)	similar to TylJ
Reference	ACC Q5SFD1 PmId [15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004) [23116432] Structural and functional studies on a 3'-epimerase involved in the biosynthesis of dTDP-6-deoxy-D-allose. (Biochemistry. , 2012) comment [PMID:15561847](2004) Chalcomycin biosynthesis gene clusterに関する文献。 chmJ(196a.a.): 3-epimerase; 6-deoxy-D-allose pathway chmJ 自体の機能実験は行われていないが、TylJとの相同性より機能を推定している模様。 --- [PMID:23116432](2012) abstract ChmJのcombined structural and functional study. ChmJがこれまで報告されていたようにdTDP-4-keto-6-deoxyglucose → dTDP-4-keto-6-deoxyalloseへの変換をする3'-epimeraseとして機能することを1H NMRを使って実証している。 dTDP-quinovoseと複合体を形成したChmJとapoenzymeの構造解析も実施。
Related Reference	ACC Q331R1 PmId [15049360] Cloning, expression, and biological function of a dTDP-deoxyglucose epimerase (gerF) gene from Streptomyces sp. GERI-155. (Biotechnol Lett. , 2004) [17053005] Biosynthesis of dTDP-6-deoxy-beta-D-allose, biochemical characterization of dTDP-4-keto-6-deoxyglucose reductase (GerKI) from Streptomyces sp. KCTC 0041BP. (Glycobiology. , 2007) comment 3rd(Q331R1) 95%, 1e-106 Streptomyces sp. KCTC 0041BP_gerF NDP-hexose-3-epimerase --- [PMID:15049360](2004) Streptomyces sp. GERI-155 = Streptomyces sp. KCTC 0041BP dTDP-deoxyglucose epimerase geneをコードすると思われるgerFをE.coliで発現、精製、機能確認。精製された酵素はdTDP-4-keto-6-deoxyglucoseからmaltolを産生し、よって発現されたタンパクがdTDP-4-keto-6-deoxyglucoseのC-3 and C-5 or C-3の異性化を触媒するdTDP-deoxyglucose epimeraseであることが確認された。 --- [PMID:17053005](2007) gerK1をクローニング、E.coliで過剰発現、機能確認。 GerK1はC-3でaxialなOH基を持つときのみ、dTDP-4-keto-6-deoxy-D-alloseの4-keto基で特異的な還元効果を示す。 GerFの存在下で、GerK1はdTDP-4-keto-6-deoxyglucose → dTDP-6-deoxy-beta-D-alloseへの変換を触媒した。このdTDP-6-deoxy-D-alloseの分離成功は、GerFがmycinose生合成経路において3,5-epimeraseではなく、3-epimeraseとして機能することを確認するのに十分な証拠を供給する。

Sequence

>dTDP-4-keto-6-deoxy-D-glucose 3-epimerase [putative 3-epimerase in D-allose pathway]
MHPLSIEGAWSQEPVIHSDHRGRSHEWFRGESFRQAFGHDFPVAQVNVAVSHRGALRGIH
YTEIPPGQAKYSVCVRGAGLDVVVDVRIGSPTFGRWEIVPMDAERNTAVYLTAGLGRAFL
SLTDDATLVYLCSSGYAPAREHSVNPLDPDLGIAWPDDIEPLLSDRDENAPTLATAERLG
LLPTYQAWQEQQQAQR

>dTDP-4-keto-6-deoxy-D-glucose 3-epimerase [putative 3-epimerase in D-allose pathway]
ATGCATCCACTCAGCATCGAGGGGGCCTGGTCGCAGGAACCCGTCATCCACTCCGACCAC
CGCGGGAGGTCCCACGAGTGGTTCCGGGGCGAGAGCTTCCGTCAGGCGTTCGGCCATGAC
TTCCCGGTGGCCCAGGTCAATGTCGCGGTGTCGCACCGCGGCGCGCTGCGCGGGATCCAC
TACACCGAGATCCCGCCAGGTCAGGCCAAGTACAGCGTGTGCGTGCGGGGAGCCGGCCTC
GACGTCGTCGTGGACGTCCGCATCGGTTCGCCGACCTTCGGGCGCTGGGAGATCGTGCCC
ATGGACGCGGAACGCAACACCGCCGTCTATCTGACCGCGGGACTGGGCCGTGCCTTCCTC
TCCCTCACCGACGACGCCACGCTCGTCTACCTCTGCTCCTCCGGATACGCTCCCGCACGC
GAGCACTCCGTCAATCCGCTCGACCCCGACCTCGGAATCGCGTGGCCGGACGACATCGAG
CCGCTGCTCTCCGACAGGGACGAGAACGCCCCGACCCTCGCGACGGCCGAACGGCTCGGT
CTGCTGCCGACCTATCAGGCCTGGCAGGAACAGCAACAGGCGCAGAGATAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000888 dTDP-4-dehydrorhamnose 3,5-epimerase-related (Family) PD001462 dTDP_sugar_isom PF00908 dTDP_sugar_isom IPR011051 RmlC-like cupin domain (Domain) SSF51182 RmlC_like_cupin IPR014710 RmlC-like jelly roll fold (Domain) G3DSA:2.60.120.10 RmlC-like_jellyroll
SignalP	No significant hit
TMHMM	No significant hit

Chalc_00120 Chalc_00140