Chalc_00120 : CDS information

Location

Organism	Streptomyces bikiniensis
Strain	NRRL 2737
Entry name	Chalcomycin
Contig	AY509120
Start / Stop / Direction	15,562 / 14,645 / - [in whole cluster] 15,562 / 14,645 / - [in contig]
Location	complement(14645..15562) [in whole cluster] complement(14645..15562) [in contig]
Type	CDS
Length	918 bp (305 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative glucose-1-phosphate thymidylyltransferase putative dTDP-glucose synthase
Product (GenBank)	putative TDP-glucose synthase
Gene
Gene (GenBank)	chmAI
EC number	2.7.7.24
Keyword	6-deoxy-D-allose (D-mycinose) D-chalcose
Note
Note (GenBank)	similar to DesIII
Reference	ACC Q5SFD2 PmId [15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004) comment Chalcomycin biosynthesis gene clusterに関する文献。 chmAI(305a.a.): TDP-glucose synthase chmAI 自体の機能実験は行われていないが、D-chalocose, D-mycinoseを生産する際の共通の中間体であるNDP-4-keto-6-deoxy-D-glucoseを生産する際にglucose-1-phosphate → NDP-glucoseの反応を司るとの記載がある。
Related Reference	ACC Q331R2 PmId [15179042] Cloning and expression of the glucose-1-phosphate thymidylyltransferase gene (gerD) from Streptomyces sp. GERI-155. (Mol Cells. , 2004) comment 2nd(Q331R2) 97%, 1e-163 Streptomyces sp. KCTC 0041BP_gerD Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24) Streptomyces sp., GERI-155 = Streptomyces sp. KCTC 0041BP gerDをE.coliで発現、精製、機能確認。 GerDはglucose-1-phosphate thymidylyltransferase活性を持ち、reversible bimolecular group transfer reactionを触媒する。正反応はdTTP and alpha-D-glucose-1-phosphateの組み合わせで最高の活性を示す。逆反応はdTDP-D-glucose and pyrophosphateに高く特異的であった。
Related Reference	ACC Q9AGY4 PmId [12147463] Homologs of the Rml enzymes from Salmonella enterica are responsible for dTDP-beta-L-rhamnose biosynthesis in the gram-positive thermophile Aneurinibacillus thermoaerophilus DSM 10155. (Appl Environ Microbiol. , 2002) comment 36th(Q9AGY4) 59%, 4e-98 Aneurinibacillus thermoaerophilus_rmlA Glucose-1-phosphate thymidylyltransferase(EC 2.7.7.24) Aneurinibacillus thermoaerophilusにおいて、Glucose-1-phosphate thymidylyltransferase (rmlA)をE. coliで発現させ、活性等の諸性質について調べている。

Sequence

>putative glucose-1-phosphate thymidylyltransferase [putative TDP-glucose synthase]
MFETPLKRPGVRGIILAGGSATRLQPLTGALSKQQLPVYDKPMIYYPLSVLMLAGIQDIL
IISSHQHVETFQVMLGDGSRLGIQLDYAVQDEPRGVADAFLVGDKHIGNDRVALILGDNV
FHGPGFSTVLKHSLRRLDGCELFGYPSKSPERYGVAEIDEQGHLLSLEEKPSRPRSNLAV
TGLYFYDNDVVELAKDLKPSARGELEITDINLSYLEQGRARLTQLGRGFAWLDMGTHDSL
LQAGQYVQLLEQRQGVRIACLEEIALRMGFIDADTCYRLGEELSASSYGDYLMEVASGLG
ATRTG

>putative glucose-1-phosphate thymidylyltransferase [putative TDP-glucose synthase]
GTGTTCGAGACACCGCTGAAACGCCCCGGAGTGAGGGGGATAATCCTCGCCGGGGGAAGC
GCCACTCGACTGCAGCCGCTGACCGGTGCGCTTTCCAAACAGCAGTTGCCCGTTTATGAC
AAACCCATGATCTATTATCCGCTGTCGGTGCTGATGCTGGCGGGAATCCAGGACATCCTC
ATCATCTCCTCGCACCAGCACGTGGAGACGTTCCAGGTCATGCTCGGCGACGGCTCGCGC
CTCGGTATCCAGCTCGACTACGCGGTCCAGGACGAGCCGCGCGGTGTCGCCGACGCGTTC
CTGGTCGGCGACAAGCACATCGGGAACGACCGGGTGGCGCTCATTCTGGGCGACAACGTC
TTCCACGGCCCCGGATTCTCCACCGTCCTGAAGCACTCCCTGCGACGTCTCGACGGATGT
GAACTGTTCGGCTATCCGTCCAAGTCGCCCGAGCGCTACGGCGTCGCCGAGATCGACGAG
CAGGGGCACCTGTTGTCCCTGGAGGAGAAGCCGAGCCGTCCTCGCTCCAACCTCGCCGTC
ACCGGGCTGTACTTCTACGACAACGACGTGGTGGAGCTGGCGAAGGACCTCAAGCCCTCA
GCCCGAGGCGAGTTGGAGATCACCGACATCAACCTGTCCTATCTGGAGCAGGGCCGCGCC
CGGCTGACCCAGCTCGGACGTGGATTCGCCTGGCTCGACATGGGAACCCACGACTCCCTG
CTCCAGGCGGGTCAGTACGTCCAGCTCCTCGAGCAGCGCCAGGGCGTACGCATCGCCTGT
CTCGAGGAGATCGCGCTGCGCATGGGCTTCATCGACGCGGACACCTGTTACCGGCTGGGT
GAGGAGCTGAGCGCCTCCAGCTACGGGGACTACCTCATGGAGGTCGCCTCGGGACTCGGG
GCGACGCGCACCGGTTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005835 Nucleotidyl transferase (Domain) PF00483 NTP_transferase IPR005907 Glucose-1-phosphate thymidylyltransferase, short form (Family) TIGR01207 RmlA
SignalP	No significant hit
TMHMM	No significant hit

Chalc_00110 Chalc_00130