Chalc_00100 Chalc_00100   Chalc_00120 Chalc_00120

Chalc_00110 : CDS information

close this sectionLocation

Organism
StrainNRRL 2737
Entry nameChalcomycin
Contig
Start / Stop / Direction14,602 / 13,631 / - [in whole cluster]
14,602 / 13,631 / - [in contig]
Locationcomplement(13631..14602) [in whole cluster]
complement(13631..14602) [in contig]
TypeCDS
Length972 bp (323 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative dTDP-glucose 4,6-dehydratase
Product (GenBank)putative TDP-glucose 4,6-dehydratase
Gene
Gene (GenBank)chmAII
EC number4.2.1.46
Keyword
  • 6-deoxy-D-allose (D-mycinose)
  • D-chalcose
Note
Note (GenBank)
  • similar to Streptomyces avermitilis SAV946 AveBII, ORF4 of the tylosin cluster
Reference
ACC
PmId
[15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004)
comment
Chalcomycin biosynthesis gene clusterに関する文献。

chmAII(323a.a.): TDP-glucose 4,6-DH

chmAII自体の機能実験は行われていないが、D-chalocose, D-mycinoseを生産する際の共通の中間体であるNDP-4-keto-6-deoxy-D-glucoseを生産する際にNDP-glucose → NDP-4-keto-6-deoxy-D-glucoseの反応を司るとの記載がある。
Related Reference
ACC
Q54116
PmId
[7883182] Cloning of the genes encoding thymidine diphosphoglucose 4,6-dehydratase and thymidine diphospho-4-keto-6-deoxyglucose 3,5-epimerase from the erythromycin-producing Saccharopolyspora erythraea. (Gene. , 1995)
comment
<dTDP-glucose 4,6-dehydratase (gdh), Saccharopolyspora erythraea, BLAST 11; id=66%>

Saccharopolyspora erythraeaより、diphospho (TDP)-glucose 4,6-dehydratase (gdh) の遺伝子をクローニングして、E. coliで発現させて活性を確認している。

close this sectionSequence

selected fasta
>putative dTDP-glucose 4,6-dehydratase [putative TDP-glucose 4,6-dehydratase]
MRVLVTGAAGFIGSHFVRQLLSGSYPELAGAHVLSLDKLTYAGNTENLAEVAGHPRHTFL
HGDICDPPTVAQALRGTDLVVHFAAESHVDRSITDSAAFVTTNVLGTQTLLRSALEAGVS
RFVHVSTDEVYGSIPEGSSTEADPLDPNSPYAASKASSDLLALAFHRTHGLDVRVTRCSN
NYGPHQHPEKVVPLFVTHLLEGLRLPLYGDGLHRRDWLHVDDHCRGIAMVAARGRAGEVY
NIGGGTELSNVDLTRRLLGVFGADWSVVDRVPDRAAHDRRYCVDTRKITEELGWAPRVAF
DRGLAETVDWYRDNGTWWKALTP
selected fasta
>putative dTDP-glucose 4,6-dehydratase [putative TDP-glucose 4,6-dehydratase]
ATGCGCGTCCTGGTGACCGGGGCAGCGGGCTTCATCGGCTCGCACTTCGTCAGACAGCTC
CTGTCGGGCTCCTATCCGGAGCTGGCCGGCGCCCACGTCCTCTCGCTGGACAAGCTCACG
TACGCCGGGAACACCGAGAACCTCGCCGAGGTCGCCGGCCACCCCCGTCACACCTTCCTG
CACGGCGACATCTGCGACCCGCCCACGGTGGCCCAGGCCCTGCGCGGCACCGACCTGGTG
GTGCACTTCGCCGCCGAGTCGCACGTGGACCGCTCGATCACGGACTCCGCCGCCTTCGTC
ACCACCAACGTGCTGGGGACGCAGACCCTGCTGCGGTCGGCGCTCGAAGCGGGCGTCAGC
CGCTTCGTCCACGTCTCCACCGACGAGGTGTACGGATCGATCCCCGAGGGCTCCTCCACC
GAGGCCGATCCGCTGGACCCGAACTCCCCCTACGCGGCGTCCAAGGCCTCCTCCGACCTC
CTGGCGCTCGCCTTCCACCGTACCCACGGCCTCGACGTCCGGGTGACGCGCTGCTCCAAC
AACTACGGCCCCCACCAGCACCCCGAGAAGGTCGTCCCGCTCTTCGTGACCCATCTGCTG
GAGGGCCTGCGCCTTCCCCTGTACGGGGACGGCCTCCACCGGCGCGACTGGCTGCACGTG
GACGACCACTGCCGGGGCATCGCCATGGTCGCCGCCCGGGGGCGGGCCGGAGAGGTGTAC
AACATCGGCGGCGGGACGGAACTGTCCAACGTCGACCTCACCCGTCGTCTTCTCGGCGTC
TTCGGTGCCGACTGGTCCGTGGTGGACCGGGTGCCGGACCGGGCCGCGCACGACCGTCGC
TACTGCGTGGACACCCGGAAGATCACCGAGGAGCTGGGATGGGCTCCCCGCGTCGCCTTC
GATCGCGGGCTCGCGGAGACCGTCGACTGGTACCGGGACAACGGCACGTGGTGGAAGGCG
CTGACACCGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001509 NAD-dependent epimerase/dehydratase (Domain)
[3-243] 5.69999999999998e-68 PF01370
PF01370   Epimerase
IPR005888 dTDP-glucose 4,6-dehydratase (Family)
[2-321] 4.30000000000003e-126 TIGR01181
TIGR01181   DTDP_gluc_dehyt
IPR016040 NAD(P)-binding domain (Domain)
[1-179] 1.20000000000001e-65 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-22] 0.753 Signal
Eukaryota   
TMHMM No significant hit
Chalc_00100 Chalc_00100   Chalc_00120 Chalc_00120
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