Chalc_00170 : CDS information

Location

Organism	Streptomyces bikiniensis
Strain	NRRL 2737
Entry name	Chalcomycin
Contig	AY509120
Start / Stop / Direction	19,049 / 20,029 / + [in whole cluster] 19,049 / 20,029 / + [in contig]
Location	19049..20029 [in whole cluster] 19049..20029 [in contig]
Type	CDS
Length	981 bp (326 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative dTDP-4-keto-6-deoxy-D-allose C-4 ketoreductase
Product (GenBank)	putative 4-ketoreductase in D-allose pathway
Gene
Gene (GenBank)	chmD
EC number
Keyword	6-deoxy-D-allose (D-mycinose)
Note
Note (GenBank)	similar to TylD and MydI
Reference	ACC Q5SFA6 PmId [15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004) comment Chalcomycin biosynthesis gene clusterに関する文献。 chmD(326a.a.): 4-ketoreductase; 6-deoxy-D-allose pathway chmD自体の機能実験は行われていないが、TylDとの相同性より4-ketoreductaseとしてNDP-6-deoxy-D-alloseの生成に関与しているとしている。
Related Reference	ACC Q331Q7 PmId [17053005] Biosynthesis of dTDP-6-deoxy-beta-D-allose, biochemical characterization of dTDP-4-keto-6-deoxyglucose reductase (GerKI) from Streptomyces sp. KCTC 0041BP. (Glycobiology. , 2007) comment <Hexose-4-ketoreductase (gerK1), Streptomyces sp. KCTC 0041BP, BLAST 2; id=93%> gerK1の遺伝子をE. coliで発現させて、その機能を調べている。 dTDP-4-keto-6-deoxy-D-alloseの4-keto carbonを、C3 positionのhydroxyl groupがaxial configurationの時のみreductionすることが示されている。 GerF (dTDP-4-keto-6-deoxyglucose 3-epimerase)存在下で以下の反応を司る。 dTDP-4-keto-6-deoxyglucose → dTDP-6-deoxy-b-D-allose

Sequence

>putative dTDP-4-keto-6-deoxy-D-allose C-4 ketoreductase [putative 4-ketoreductase in D-allose pathway]
MTADRWAGRTVLVTGALGFIGSHFVRQLEARGAEVLALYRTERPQLQAELAALDRVRLIR
TELRDESDVRGAFKYLAPSIDTVVHCAAMDGNAQFKLERSAEILDSNQRTISHLLNCVRD
FGVGEAVVMSSSELYCAPPTAAAHEDDDFRRSMRYTDNGYVLSKTYGEILARLHREQFGT
NVFLVRPGNVYGPGDGYDPSRGRVIPSMLAKADAGEEIEIWGDGSQTRSFIHVTDLVRAS
LRLLETGKYPEMNVAGAEQVSILELARMVMAVLGRPERIRLDPGRPVGAPSRLLDLTRMS
EVIDFEPQPLRTGLEETARWFRHHTR

>putative dTDP-4-keto-6-deoxy-D-allose C-4 ketoreductase [putative 4-ketoreductase in D-allose pathway]
GTGACCGCTGACCGCTGGGCCGGCCGCACGGTGCTCGTCACGGGAGCACTCGGGTTCATC
GGCTCCCACTTCGTCCGACAGCTGGAGGCGCGCGGAGCCGAGGTGCTCGCCCTCTACCGC
ACCGAACGGCCGCAATTACAGGCCGAGTTGGCCGCGCTCGACCGAGTACGCCTGATCAGG
ACGGAGCTGCGGGACGAGTCGGACGTGCGAGGGGCCTTCAAGTACCTGGCACCCTCCATC
GACACCGTCGTCCACTGCGCGGCCATGGACGGCAACGCGCAGTTCAAGCTGGAGCGCTCG
GCCGAGATCCTCGACAGCAACCAGCGGACCATCTCCCACCTGCTGAACTGCGTACGGGAC
TTCGGCGTCGGCGAGGCCGTGGTCATGAGCTCCTCCGAGCTGTACTGCGCGCCGCCCACC
GCGGCGGCCCACGAGGACGACGACTTCCGCCGATCCATGCGGTACACGGACAACGGCTAC
GTCCTGTCCAAGACCTACGGCGAGATCCTGGCCAGGCTCCACCGCGAGCAGTTCGGCACC
AACGTCTTCCTGGTGCGACCGGGCAACGTCTACGGGCCGGGAGACGGCTACGACCCCTCC
CGGGGCCGGGTGATCCCCAGCATGCTGGCCAAGGCCGACGCCGGCGAGGAGATAGAGATC
TGGGGGGACGGCAGTCAGACCCGGTCCTTCATCCACGTCACCGACCTGGTACGGGCCTCA
CTGCGCCTGCTGGAGACCGGCAAGTACCCCGAGATGAACGTGGCCGGCGCGGAACAGGTC
TCCATCCTGGAGCTCGCCCGGATGGTGATGGCCGTCCTGGGACGGCCCGAGCGCATCCGC
CTCGACCCCGGCCGCCCCGTCGGCGCCCCGAGCAGACTTCTGGATCTGACCAGGATGTCG
GAAGTGATCGACTTCGAGCCCCAGCCCCTGCGGACCGGGCTGGAAGAGACCGCTCGCTGG
TTCCGCCACCACACGCGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	Eukaryota Bacteria, Gram-positive
TMHMM	No significant hit

Chalc_00160 Chalc_00180