Chalc_00180 : CDS information

Location

Organism	Streptomyces bikiniensis
Strain	NRRL 2737
Entry name	Chalcomycin
Contig	AY509120
Start / Stop / Direction	20,062 / 21,273 / + [in whole cluster] 20,062 / 21,273 / + [in contig]
Location	20062..21273 [in whole cluster] 20062..21273 [in contig]
Type	CDS
Length	1,212 bp (403 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative hexose C-2'' O-methyltransferase
Product (GenBank)	putative 2'OH-methyltransferase in D-mycinose pathway
Gene	chmMI chmE
Gene (GenBank)	chmMI
EC number	2.1.1.-
Keyword	D-mycinose
Note
Note (GenBank)	member of O-methyltransferase homology family, including TylD and MydI
Reference	ACC Q5SFA5 PmId [15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004) comment Chalcomycin biosynthesis gene clusterに関する文献。論文中ではchmMIでは無く、chmEとなっている。 chmE(403a.a.): 2-O-methyltransferase; D-mycinose pathway chmE 自体の機能実験は行われていないが、TylEとの相同性より2-O-methyltransferaseとして、同じく methyltransferaseとして機能すると推定しているchmFと共に、5-O-chalcosyl-20-O-(6-deoxyallosyl)-macrolactoneより5-O-chalcosyl-20-O-mycinosyl-macrolactoneを生じると提唱している。
Related Reference	ACC Q83WF2 PmId [19415708] Functional analysis of MycE and MycF, two O-methyltransferases involved in the biosynthesis of mycinamicin macrolide antibiotics. (Chembiochem. , 2009) comment <Methyltransferase (mycE), Micromonospora griseorubida, BLAST 6; id=65%> mycinamicin生合成における2つのO-methyltransferase、MycE と MycFの機能を解析している。 mycE, mycFそれぞれの遺伝子をHis-tagを付けてE. coliで発現させて精製し、各々の機能を調べている。各酵素はS-adenosyl-L-methionine (SAM)-dependent deoxysugar methyltransferaseであることが確認された。MycEの最適反応には、MycFよりも明白にMg(2+)が必要。 MycE → aglycone付着6-deoxyalloseのC2''-OHをmethylation = javose MycF → aglycone付着javoseのC3''-OHをmethylation = mycinose

Sequence

>putative hexose C-2'' O-methyltransferase [putative 2'OH-methyltransferase in D-mycinose pathway]
MVTHAPNSLISDIIRASGGHDADLKDLAARHDPADIVRVLLDEITSRCPAPVNDVPVLVE
LAVRAGDRLFPTYLYVLKGGPVRLAAKDEAFVAMRVEYELGELARELFGPVRENVTGVRG
TTLFPYVGDTASEGEEDSGAEHIGTHFLAAQQGSQTVLAGCHSHKPDLSELSSRYLTPKW
GSLHWFTPHYDRHFRSYRDQPVRVLEIGIGGYKHPEWGGGSLRMWKHFFHRGEIYGLDIV
DKSHFDAPRITTLRGDQSDPDHLRSIAEKYGPFDIVIDDGSHINDHIRTSFQALFPHVRP
GGLYVIEDLWTAYWSGFGGDEDPKRYSGTSLGLLKSLVDSIQHEELPEAGDHRPSYADQH
VVGMHLYHNLAFIEKGTNAEGGIPPWIPRDFETLVAVSSGGHA

>putative hexose C-2'' O-methyltransferase [putative 2'OH-methyltransferase in D-mycinose pathway]
GTGGTCACACACGCCCCGAACTCGCTGATCAGTGACATAATCCGCGCCTCCGGCGGGCAT
GACGCCGACCTCAAGGACCTGGCCGCCCGACACGATCCGGCCGACATCGTCCGCGTACTC
CTGGACGAGATCACCTCACGCTGCCCCGCTCCCGTGAACGACGTCCCCGTCCTCGTCGAG
CTGGCCGTCCGGGCGGGAGACCGCCTCTTCCCCACCTATCTGTACGTCCTCAAGGGCGGC
CCGGTGCGCCTCGCGGCCAAGGACGAGGCGTTCGTCGCCATGCGCGTCGAGTACGAGCTG
GGCGAACTGGCCCGCGAGCTGTTCGGACCGGTGCGGGAGAACGTCACCGGCGTCCGCGGA
ACGACTCTCTTCCCCTACGTCGGGGACACGGCGTCGGAAGGCGAGGAGGACTCGGGTGCC
GAGCACATCGGCACGCACTTCCTGGCCGCGCAGCAGGGCTCCCAGACCGTGCTCGCCGGC
TGCCATTCCCACAAGCCGGACCTCAGCGAGCTCTCCTCGCGCTACCTCACCCCGAAGTGG
GGCTCGCTGCACTGGTTCACCCCCCACTACGACCGCCACTTCAGGAGCTACCGGGACCAG
CCCGTACGCGTTCTGGAGATCGGCATCGGCGGCTACAAGCACCCCGAGTGGGGCGGCGGC
TCCCTGCGCATGTGGAAGCACTTCTTCCATCGCGGCGAGATCTACGGCCTGGACATCGTC
GACAAGTCGCACTTCGACGCGCCGCGCATCACGACCCTGCGCGGCGACCAGAGCGACCCC
GACCACCTGCGGTCGATCGCCGAGAAGTACGGACCGTTCGACATCGTCATCGACGACGGA
AGCCACATCAACGACCACATCCGGACCTCGTTCCAGGCACTGTTCCCGCATGTGCGGCCC
GGCGGCCTCTACGTGATCGAGGACCTGTGGACCGCCTACTGGTCCGGCTTCGGCGGCGAC
GAGGACCCGAAGCGGTACAGCGGGACGAGCCTGGGCCTGCTCAAGTCCCTCGTCGACTCG
ATCCAGCACGAGGAACTGCCGGAGGCCGGCGACCACCGTCCCAGTTACGCGGACCAGCAC
GTGGTCGGCATGCACCTCTACCACAACCTGGCGTTCATCGAGAAGGGCACCAACGCCGAG
GGCGGCATCCCCCCGTGGATCCCACGCGACTTCGAGACCCTCGTCGCCGTCTCCTCCGGG
GGCCACGCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	No significant hit
TMHMM	No significant hit

Chalc_00170 Chalc_00190