Chalc_00190 : CDS information

Location

Organism	Streptomyces bikiniensis
Strain	NRRL 2737
Entry name	Chalcomycin
Contig	AY509120
Start / Stop / Direction	21,329 / 22,585 / + [in whole cluster] 21,329 / 22,585 / + [in contig]
Location	21329..22585 [in whole cluster] 21329..22585 [in contig]
Type	CDS
Length	1,257 bp (418 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative glycosyltransferase
Product (GenBank)	putative D-allose glycosyltransferase
Gene
Gene (GenBank)	chmN
EC number
Keyword	6-deoxy-D-allose (D-mycinose)
Note
Note (GenBank)	similar to TylN, MycD
Reference	ACC Q5SFA4 PmId [15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004) comment Chalcomycin biosynthesis gene clusterに関する文献。 chmN(418a.a.): 6-deoxy-D-allosyltransferase chmN自体の機能実験は行われていないが、6-deoxy-D-alloseをmacrolactoneに結合させるのに必要であると記載されている。
Related Reference	ACC Q331Q5 PmId [20069384] Biosynthesis of dihydrochalcomycin: characterization of a deoxyallosyltransferase (gerGTI). (Mol Cells. , 2010) comment 2nd(Q331Q5) 95%. 0.0 Streptomyces sp. KCTC 0041BP_gerTI 6-deoxy-D-allosyltransferase [PMID:20069384](2010)abstract おそらくgerTI = gerT1 = gerGTI 著者が同じなのに年代も前後して定まっていない。 gerGTIの破壊、その相補による産物測定。 gerGTIは、GerGTIIの後に働くdeoxyallosyltransferaseをコードすることが中間体から示されている。
Related Reference	ACC O70023 PmId [9651492] Characterization and targeted disruption of a glycosyltransferase gene in the tylosin producer, Streptomyces fradiae. (Gene. , 1998) comment 4th(O70023) 67%, 1e-154 Streptomyces fradiae_tylN Deoxyallosyl-transferase tylN破壊株において、demycinosyl-tylosinが生産された。一方、tylosin biosynthesisに関与する他のglycosyltransferase activitiesに影響は無かったため、tylNはdeoxyallosyl transferaseをコードしていると示している。

Sequence

>putative glycosyltransferase [putative D-allose glycosyltransferase]
MRVTLLSVGSRGDVQPFVALGIGLKARGHDVTLAAPATLRPLVERAGLTYRLSPGDPDGF
FTMPEVVEALRRGPSFKNMLAGMPEAPESYTQQVVDAIHDAAEGADLIVNAPLTLAAAYG
HPPAPWASVSWWPNSMTSAFPAVESGQRHLGPLTSLYNRYTHRRAARDEWEWRRPEIDGY
RRRLGLRPFGDESPFLRLGHDRPYLFPFSPSVLPKPRDWPRQSHVTGYWFWDQHGEPPAE
LESFLEDGEPPVALTFGSTWSLHRQEEALQAALDAVRGVGRRLVMVDGPDSDLPDDVLRL
HQVDYATLFPRMAAVIHHGGAGTTAEVLRAGVPQVIVPVFADHPFWAARLSRTGVAARPV
PFARFSREALAQSVRQAVTDPAMAGRARRLGERVSKERGVDTACDILEKWAETARATA

>putative glycosyltransferase [putative D-allose glycosyltransferase]
ATGCGCGTGACCCTGCTGAGCGTCGGATCCCGAGGCGACGTCCAGCCGTTCGTCGCCCTC
GGCATCGGCCTCAAGGCCCGCGGCCACGACGTCACCCTGGCCGCCCCCGCCACGCTGCGG
CCACTGGTCGAGCGCGCGGGACTGACGTACAGGCTGTCCCCCGGGGATCCCGACGGATTC
TTCACCATGCCCGAGGTCGTCGAAGCGCTGCGGCGCGGCCCCTCGTTCAAGAACATGCTC
GCGGGGATGCCCGAGGCGCCCGAGAGCTACACACAGCAGGTCGTCGACGCGATCCACGAC
GCCGCCGAGGGCGCCGACCTCATAGTGAACGCGCCCCTCACCCTGGCCGCCGCGTACGGG
CACCCGCCCGCCCCGTGGGCCTCGGTGTCCTGGTGGCCCAACAGCATGACCTCGGCCTTC
CCGGCCGTCGAATCCGGGCAGCGCCACCTCGGACCGCTGACGTCCCTGTACAACCGCTAC
ACCCATCGCAGGGCGGCACGCGACGAGTGGGAGTGGCGACGCCCCGAGATCGACGGCTAC
CGCCGACGCCTCGGCCTCCGGCCCTTCGGCGACGAGTCCCCGTTCCTCCGACTGGGGCAC
GACCGCCCGTACCTGTTCCCCTTCAGCCCCAGCGTGCTGCCCAAGCCGCGGGACTGGCCG
CGCCAGAGCCACGTCACCGGCTACTGGTTCTGGGACCAGCACGGGGAGCCGCCCGCCGAG
CTGGAGTCGTTCCTGGAGGACGGGGAGCCCCCGGTGGCGCTCACCTTCGGCAGCACCTGG
TCACTCCACCGGCAGGAGGAGGCCCTCCAGGCCGCCCTCGACGCCGTCCGTGGCGTCGGA
CGCCGACTGGTCATGGTCGACGGACCGGACAGCGACCTGCCCGACGACGTGCTCCGCCTG
CACCAGGTGGACTACGCCACCCTCTTCCCCAGGATGGCCGCGGTGATCCACCACGGCGGC
GCCGGCACGACCGCCGAGGTCCTCCGGGCCGGAGTGCCCCAGGTCATCGTGCCGGTCTTC
GCCGATCACCCCTTCTGGGCGGCTCGACTGTCCAGAACAGGCGTCGCCGCCCGGCCGGTC
CCCTTCGCCCGCTTCAGCCGAGAGGCACTGGCGCAGAGCGTCCGCCAGGCGGTCACCGAT
CCCGCGATGGCGGGCCGGGCCAGGCGACTGGGCGAACGGGTCTCCAAGGAACGGGGAGTG
GACACCGCCTGCGACATCCTCGAGAAGTGGGCGGAGACGGCACGCGCCACGGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002213 UDP-glucuronosyl/UDP-glucosyltransferase (Family) PF00201 UDPGT IPR004276 Glycosyl transferase, family 28 (Domain) PF03033 Glyco_transf_28
SignalP	Eukaryota
TMHMM	No significant hit

Chalc_00180 Chalc_00200