Chalc_00200 : CDS information

Location

Organism	Streptomyces bikiniensis
Strain	NRRL 2737
Entry name	Chalcomycin
Contig	AY509120
Start / Stop / Direction	23,495 / 22,653 / - [in whole cluster] 23,495 / 22,653 / - [in contig]
Location	complement(22653..23495) [in whole cluster] complement(22653..23495) [in contig]
Type	CDS
Length	843 bp (280 aa)

Annotation

Category	4.4 resistance
Product	putative 23S rRNA methyltransferase
Product (GenBank)	putative resistance determinant
Gene
Gene (GenBank)	chrB
EC number	2.1.1.-
Keyword
Note
Note (GenBank)	member of predicted 23S-rRNA G748-methyltransferase family; similar to TlrB
Reference	ACC Q5SFA3 PmId [15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004) comment Chalcomycin biosynthesis gene clusterに関する文献。 chrB(280a.a.): 23S-rRNA G748-methyltransferase chrB自体の機能実験は行われていないが、tlrBとの相同性により機能を推定している。
Related Reference	ACC Q9S1M6 PmId [10348045] Molecular analysis of tlrB, an antibiotic-resistance gene from tylosin-producing Streptomyces fradiae, and discovery of a novel resistance mechanism. (J Antibiot (Tokyo). , 1999) comment BLAST 6; id=59% Streptomyces fradiae_rlmAII(tlrB) 23S rRNA (guanine(748)-N(1))-methyltransferase(EC 2.1.1.188) TlrB遺伝子をE. coliにおいてmalEとfusionさせて発現させ、S-adenosyl-methionine (SAM)存在下でmacrolidesを不活化することを確認し、tylosin耐性に関与していることを示唆している。

Sequence

>putative 23S rRNA methyltransferase [putative resistance determinant]
MLNRIVRYLACPHCGASLAQGDRALFCPAGHSFDIAKQGYVNLLPRATKLRADTKEMVEA
RDAFLSAGHYDPVMDALVDLARRTADPAVPGCVVDIGGGTGHYHAGVMEAFPDAQGLLLD
ISKYAVRRAAKAHPRIAAAVTDAWQTLPLRDAAAGMVINTFAPRNGPELHRVLHPRGVLL
VVTPLPDHLREVIGALGLLQVDEGKESRLAEQLAPHFSAVATEELTRTMALDHQALAHLV
GMGPNAWHRDAQRDLETIQRLPAPTRVTLSVRLSAYRLSA

>putative 23S rRNA methyltransferase [putative resistance determinant]
ATGCTCAACAGGATCGTGCGCTACCTCGCCTGCCCGCACTGCGGTGCTTCGCTGGCTCAG
GGCGACCGCGCACTTTTCTGCCCCGCCGGACACTCCTTCGACATCGCGAAGCAGGGTTAT
GTGAATCTGCTTCCCAGGGCGACGAAGCTGCGGGCCGACACCAAGGAAATGGTGGAGGCC
CGGGACGCATTCCTGTCGGCGGGTCACTACGACCCCGTGATGGATGCGCTGGTCGATCTG
GCGCGGCGGACGGCCGATCCGGCCGTGCCCGGCTGTGTGGTCGACATCGGTGGGGGGACG
GGCCACTATCACGCCGGGGTCATGGAAGCGTTCCCCGATGCCCAGGGCCTGCTGCTGGAC
ATCTCCAAGTACGCCGTGCGGCGTGCCGCGAAGGCGCATCCGCGGATCGCGGCCGCGGTG
ACCGACGCCTGGCAGACGCTTCCGCTGCGGGATGCCGCAGCCGGCATGGTGATCAACACG
TTCGCTCCCCGCAACGGTCCTGAACTGCATCGCGTTCTCCATCCCCGTGGCGTTCTCCTG
GTTGTCACTCCTCTGCCCGATCACCTGCGGGAGGTGATCGGCGCCCTCGGCCTGCTGCAG
GTGGACGAAGGCAAGGAGTCGCGTCTCGCGGAGCAGCTCGCTCCGCACTTCTCGGCCGTC
GCCACGGAGGAGTTGACCCGGACCATGGCTCTCGACCACCAGGCTCTGGCGCACCTGGTC
GGCATGGGGCCCAATGCCTGGCACCGTGACGCGCAGCGGGATCTGGAGACGATCCAGCGG
CTGCCGGCGCCGACTCGGGTCACGCTCTCCGTGCGGCTCTCCGCCTACCGGTTGTCGGCG
TAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001077 O-methyltransferase, family 2 (Domain) PF00891 Methyltransf_2 IPR016718 rRNA (guanine-N1-)-methyltransferase A, predicted (Family) PIRSF018249 MyrA_prd
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Chalc_00190 Chalc_00210