Chalc_00270 Chalc_00270   Chalc_00290 Chalc_00290

Chalc_00280 : CDS information

close this sectionLocation

Organism
StrainNRRL 2737
Entry nameChalcomycin
Contig
Start / Stop / Direction66,194 / 66,940 / + [in whole cluster]
66,194 / 66,940 / + [in contig]
Location66194..66940 [in whole cluster]
66194..66940 [in contig]
TypeCDS
Length747 bp (248 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative oxidoreductase
Product (GenBank)putative post-PKS ketoreductase
Gene
Gene (GenBank)chmU
EC number
Keyword
Note
Note (GenBank)
  • similar to putative 3-oxoacyl-acyl carrier protein reductase family; similar to SimJ2, NovJ, and Pseudomonas putida PP2783
Reference
ACC
PmId
[15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004)
comment
Chalcomycin biosynthesis gene clusterに関する文献。

chmU(248a.a.): 3-ketoreductase

chmU自体の機能実験は行われていないが、chalcomycinのmacrolactoneコンポーネントに2,3-double bondを導入する役割をしていると予測されている。同時に必要とされる2,3-dehydratase候補をコードする遺伝子はchm cluster内に見られない。
Related Reference
ACC
P51831
PmId
[8759840] Bacillus subtilis acyl carrier protein is encoded in a cluster of lipid biosynthesis genes. (J Bacteriol. , 1996)
comment
Blast 81st, id41%, 5e-49
B.subtilis_fabG
3-oxoacyl-[acyl-carrier-protein] reductase FabG(EC 1.1.1.100)

E. coli_fabD mutantを利用して、B.subtilisのfatty acid biosynthetic gene(plsX, fabD, fabG, acpP)をクローニング。

close this sectionSequence

selected fasta
>putative oxidoreductase [putative post-PKS ketoreductase]
MEFEGQVALVTGAGRGIGRATVVRLAEAGCDIALHYNQAKAQAEEVAERIAALGRTVELF
PGDLSRPETGRQLVAAVQQKFDRIDILVNSAGITRDKLLLSMEADDIHQVIATNLVGPMF
LTQAVALTMLRQRSGRIVNISSAAASRPGKGQSNYAASKAGLEAFTRAMAVELGSRGILV
NAVAPGIVKTGLTEALREGAEPELLARQVIGSFAEPEAVAEAVAYLASPRNTHTTGTVLT
VDGGLKMV
selected fasta
>putative oxidoreductase [putative post-PKS ketoreductase]
ATGGAATTCGAAGGTCAGGTCGCGCTCGTCACCGGGGCCGGCAGGGGGATCGGCCGTGCG
ACGGTCGTCCGCCTCGCGGAGGCCGGATGTGACATCGCCCTCCACTACAACCAAGCGAAA
GCGCAGGCCGAGGAAGTCGCCGAGCGCATCGCCGCACTGGGCCGCACGGTCGAACTGTTC
CCGGGCGACCTCTCCCGCCCCGAGACCGGGCGACAGCTCGTGGCCGCGGTGCAGCAGAAG
TTCGACCGGATCGACATCCTGGTGAACAGCGCGGGCATCACACGGGACAAACTCCTGCTG
TCCATGGAGGCGGACGACATCCACCAGGTCATCGCCACCAACCTCGTCGGCCCGATGTTC
CTCACCCAGGCGGTCGCGCTCACCATGCTGCGTCAACGCTCCGGGCGCATCGTCAACATC
TCCTCCGCCGCCGCGAGCAGGCCCGGAAAGGGCCAGTCCAACTACGCCGCGTCCAAGGCC
GGTCTGGAGGCCTTCACCAGGGCCATGGCGGTGGAACTCGGATCCCGCGGAATTCTCGTC
AACGCGGTCGCTCCCGGCATCGTCAAGACCGGCCTGACCGAGGCTCTCCGCGAGGGGGCG
GAGCCCGAACTCCTGGCCCGGCAGGTGATCGGTTCCTTCGCCGAACCCGAGGCGGTGGCG
GAGGCGGTGGCCTACTTGGCGAGCCCGCGCAACACGCACACGACGGGCACGGTCCTCACC
GTCGACGGCGGGCTCAAGATGGTGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[82-93] 7.30000590915208e-14 PR00080 [135-143] 7.30000590915208e-14 PR00080 [155-174] 7.30000590915208e-14 PR00080
PR00080   SDRFAMILY
[7-173] 1.5e-39 PF00106
PF00106   adh_short
IPR002347 Glucose/ribitol dehydrogenase (Family)
[7-24] 1.10000150671643e-42 PR00081 [82-93] 1.10000150671643e-42 PR00081 [129-145] 1.10000150671643e-42 PR00081 [155-174] 1.10000150671643e-42 PR00081 [176-193] 1.10000150671643e-42 PR00081 [209-229] 1.10000150671643e-42 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[2-246] 9.9e-83 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
[142-170] PS00061
PS00061   ADH_SHORT
SignalP No significant hit
TMHMM No significant hit
Chalc_00270 Chalc_00270   Chalc_00290 Chalc_00290
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