Chalc_00300 Chalc_00300   Chalc_00320 Chalc_00320

Chalc_00310 : CDS information

close this sectionLocation

Organism
StrainNRRL 2737
Entry nameChalcomycin
Contig
Start / Stop / Direction71,146 / 70,193 / - [in whole cluster]
71,146 / 70,193 / - [in contig]
Locationcomplement(70193..71146) [in whole cluster]
complement(70193..71146) [in contig]
TypeCDS
Length954 bp (317 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative S11 family peptidase
Product (GenBank)putative D-alanyl-D-alanine carboxypeptidase
Gene
Gene (GenBank)ORF6
EC number
Keyword
Note
  • It is supposed that this ORF is not involved in meilingmycin biosynthesis.
  • The N-terminal position was modified from original INSDC entry.
Note (GenBank)
  • similar to Streptomyces coelicolor SC4439, and Streptomyces avermitilis SAV3781
Reference
ACC
PmId
[15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004)
comment
Chalcomycin biosynthesis gene clusterに関する文献。

ORF6(231a.a.): D-alanyl-D-alanine carboxypeptidase
ORF6自体の機能実験は無し。
Related Reference
ACC
P33013
PmId
[8955390] dacD, an Escherichia coli gene encoding a novel penicillin-binding protein (PBP6b) with DD-carboxypeptidase activity. (J Bacteriol. , 1996)
comment
126th(P33013) 34%, 7e-15
E.coli_dacD(phsE, yeeC)
D-alanyl-D-alanine carboxypeptidase dacD(EC 3.4.16.4)

クローニングして、酵素を精製、酵素活性、penicillin bindingも確かめている。

close this sectionSequence

selected fasta
>putative S11 family peptidase [putative D-alanyl-D-alanine carboxypeptidase]
MSLPRTPRHRLVGGALSVALLTALGITVASPSGAPPAAGGERLPVSDIGPLPWPQDGQSA
VELEGTGSLGERGERRPVPIASLTKVMTAYVILEEHPLRPDEDGPLIEVDRTAAHEAGVG
GESTVPVRSGERHSQRRLLELLLIPSGNNVARLLARWDSGSQAAFVTKMRRAAVRLGMKD
TVYTGASGIEPTTTSTAADQVRLTRAAMEDPVFRAVVATRETTVPGLGTITNTNPLLDTP
GVLGVKTGSSTPAGGNLLWAYEVRVGGAPRLLVGAVLHQRANTTPAEGLRAAVEAARGLL
TAVRERLAAAGTQGGER
selected fasta
>putative S11 family peptidase [putative D-alanyl-D-alanine carboxypeptidase]
GTGTCCCTGCCCCGCACCCCCCGTCACCGCCTCGTCGGCGGTGCCCTGTCCGTGGCCCTG
CTGACGGCCCTGGGCATCACCGTCGCCTCGCCCTCCGGCGCGCCTCCGGCGGCGGGAGGC
GAGCGACTACCGGTCTCCGACATCGGGCCGCTCCCCTGGCCGCAGGACGGCCAGAGCGCC
GTCGAGCTCGAAGGAACCGGCTCGCTGGGCGAGCGGGGCGAGCGGCGGCCCGTACCGATC
GCGAGCCTGACGAAGGTGATGACCGCCTACGTCATCCTCGAAGAGCATCCGCTGAGGCCC
GACGAGGACGGCCCCCTGATCGAAGTGGACCGGACGGCCGCGCACGAGGCGGGGGTCGGC
GGCGAGTCGACCGTCCCGGTACGGTCCGGCGAACGCCACTCCCAGCGCCGGCTCCTGGAA
CTGCTCCTCATCCCCTCCGGCAACAACGTCGCCCGTCTGCTGGCCCGCTGGGACTCCGGC
AGCCAGGCGGCCTTCGTGACGAAGATGCGGCGCGCCGCCGTACGGCTCGGGATGAAGGAC
ACGGTCTACACGGGGGCGAGCGGCATCGAGCCGACGACCACAAGCACCGCGGCCGACCAG
GTGCGGCTGACGCGGGCCGCGATGGAGGACCCTGTCTTCCGGGCGGTCGTCGCCACCCGC
GAGACCACCGTCCCGGGCCTCGGGACGATCACCAACACCAATCCCCTGCTGGACACCCCG
GGCGTCCTCGGTGTGAAGACGGGCTCCAGCACCCCGGCGGGCGGGAACCTCCTGTGGGCC
TACGAGGTGCGCGTGGGCGGCGCGCCCCGGCTGCTGGTGGGCGCCGTCCTGCACCAGCGG
GCGAACACGACGCCCGCCGAGGGGCTCCGGGCCGCGGTCGAGGCGGCCCGTGGCCTGCTC
ACCGCCGTCCGCGAGCGGCTGGCCGCCGCCGGAACCCAGGGCGGGGAACGATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001967 Peptidase S11, D-alanyl-D-alanine carboxypeptidase A, N-terminal (Domain)
[68-258] 9.1e-31 PF00768
PF00768   Peptidase_S11
IPR012338 Beta-lactamase/transpeptidase-like (Domain)
[70-263] 3.3e-46 G3DSA:3.40.710.10
G3DSA:3.40.710.10   no description
[7-307] 2.2e-40 SSF56601
SSF56601   beta-lactamase/transpeptidase-like
IPR018044 Peptidase S11, D-alanyl-D-alanine carboxypeptidase A (Family)
[81-92] 6.9e-13 PR00725 [138-155] 6.9e-13 PR00725 [165-178] 6.9e-13 PR00725
PR00725   DADACBPTASE1
SignalP
[1-34] 1 Signal
Bacteria, Gram-positive   
[1-39] 0.998 Signal
Bacteria, Gram-negative   
[1-29] 0.962 Signal
Eukaryota   
TMHMM
[12-34] Transmembrane (i-o)
Transmembrane 1   
Chalc_00300 Chalc_00300   Chalc_00320 Chalc_00320
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