Chalc_00320 : CDS information

Location

Organism	Streptomyces bikiniensis
Strain	NRRL 2737
Entry name	Chalcomycin
Contig	AY509120
Start / Stop / Direction	72,542 / 71,382 / - [in whole cluster] 72,542 / 71,382 / - [in contig]
Location	complement(71382..72542) [in whole cluster] complement(71382..72542) [in contig]
Type	CDS
Length	1,161 bp (386 aa)

Annotation

Category	5.1 general function
Product	putative two-component system histidine kinase
Product (GenBank)	sensory histidine kinase
Gene
Gene (GenBank)	ORF7
EC number
Keyword
Note	It is supposed that this ORF is not involved in chalcomycin biosynthesis.
Note (GenBank)	similar to Streptomyces coelicolor SC5304, SC3359 and Streptomyces avermitilis SAV2950 and SAV4703
Reference	ACC Q5SFB9 PmId [15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004) comment Chalcomycin biosynthesis gene clusterに関する文献。 ORF7(386a.a.): Sensory histidine kinase
Related Reference	ACC Q9ZEP3 PmId [10411727] A putative two-component signal transduction system regulates sigmaE, a sigma factor required for normal cell wall integrity in Streptomyces coelicolor A3(2). (Mol Microbiol. , 1999) comment 20th(Q9ZEP3) 54%, 8e-59 Streptomyces coelicolor_cseC Sensor protein CseC(EC 2.7.13.3) cseB null mutant作成あり。 CseB/CseC two-component systemはcell envelopeからのシグナルに応答してsigE promoterの活性を調節する。
Family/Domain	FD IPR005467, IPR003661 comment [IPR005467] Signal transduction histidine kinase, core (Domain) [IPR003661] Signal transduction histidine kinase, subgroup 1, dimerisation/phosphoacceptor domain (Domain)

Sequence

>putative two-component system histidine kinase [sensory histidine kinase]
MALTVGVLVHDSVLRRSLHEGAGRAHVALATALEDADAAGEEGPRVSPEELPEALLRQIE
HGGEATLYEDGPPAPVFRAARREGGKLYAVEVDMTADLLTRQALDRHMWKYSLLTLGVVV
PATALATELPARRLRRVARTARRITAGDLDARTGTVRGGDEVAEISAVVDSMADSLQQRI
DTEQRFTADVAHELRTPLMGLVTSAELLPEGEVTDMVQSRVRVLRDLVEDLLEVSRLDAG
AETAQQGPVDLGALVRDSVARTGLAAQVTRQGAAVVESDPRRLDRIVSNLVVNAHRHGAG
RVEVTVAGRTVTVRDHGPGFPADLLSHGPQRFRTGSAERGHGHGLGLTIASGQARVIGAT
LAFANARDGGAVATLSLPEDGQTSEA

>putative two-component system histidine kinase [sensory histidine kinase]
ATGGCGCTGACCGTCGGAGTGCTGGTGCACGACTCGGTGCTGCGGCGCTCCCTGCACGAG
GGCGCGGGCAGGGCCCACGTGGCGCTGGCAACGGCCTTGGAGGACGCCGACGCAGCAGGT
GAGGAAGGGCCCCGGGTGTCCCCCGAGGAACTGCCCGAGGCCCTGCTGAGGCAGATCGAG
CACGGCGGCGAGGCCACCCTCTACGAGGACGGGCCCCCGGCCCCCGTCTTCCGGGCGGCC
CGCCGGGAGGGCGGGAAGCTGTACGCGGTCGAGGTCGACATGACCGCCGACCTGCTGACC
CGGCAGGCCCTGGACCGTCACATGTGGAAGTACTCGCTGCTCACCCTGGGCGTCGTCGTC
CCCGCCACGGCCCTGGCGACGGAACTCCCCGCCCGGCGGCTGCGCCGGGTCGCCCGCACG
GCCCGGCGCATCACGGCGGGCGACCTCGACGCCCGGACGGGAACGGTCCGCGGAGGCGAC
GAGGTCGCCGAGATCTCGGCCGTGGTCGACTCGATGGCCGACAGCCTCCAGCAGCGGATC
GACACCGAGCAGCGCTTCACCGCCGACGTCGCACACGAGCTGCGCACCCCGCTGATGGGC
CTGGTCACCTCGGCGGAACTGCTGCCCGAGGGCGAGGTGACGGACATGGTCCAGAGCCGG
GTACGGGTGCTGCGCGACCTGGTGGAGGACCTCCTGGAGGTCTCCCGGCTCGACGCGGGC
GCCGAGACGGCCCAGCAGGGACCGGTCGACCTGGGCGCCCTCGTCCGGGACTCGGTGGCG
CGGACGGGCCTCGCGGCGCAGGTGACGCGACAGGGGGCGGCAGTGGTCGAGTCGGACCCC
CGGCGCCTGGACCGCATCGTCTCCAACCTCGTCGTCAACGCCCACCGGCACGGCGCGGGT
CGGGTGGAGGTCACGGTCGCCGGCCGGACCGTGACCGTCCGGGACCACGGCCCCGGCTTC
CCCGCCGACCTGCTGTCCCACGGCCCCCAGCGGTTCCGCACGGGCTCGGCCGAACGGGGG
CACGGGCACGGCCTTGGCCTGACGATCGCGTCCGGCCAGGCGAGGGTGATCGGCGCGACC
CTGGCCTTCGCCAACGCCCGGGACGGAGGAGCGGTCGCGACCCTGAGCCTGCCCGAGGAC
GGGCAGACGTCCGAGGCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003594 ATPase-like, ATP-binding domain (Domain) G3DSA:3.30.565.10 ATP_bd_ATPase PF02518 HATPase_c SM00387 HATPase_c SSF55874 ATP_bd_ATPase IPR003660 HAMP linker domain (Domain) PF00672 HAMP PS50885 HAMP SM00304 HAMP IPR003661 Signal transduction histidine kinase, subgroup 1, dimerisation/phosphoacceptor domain (Domain) SM00388 HisKA PF00512 HisKA IPR005467 Signal transduction histidine kinase, core (Domain) PS50109 HIS_KIN IPR009082 Signal transduction histidine kinase, homodimeric (Domain) SSF47384 His_kin_homodim
SignalP	No significant hit
TMHMM	No significant hit

Chalc_00310 Chalc_00330