Chalc_00330 : CDS information

Location

Organism	Streptomyces bikiniensis
Strain	NRRL 2737
Entry name	Chalcomycin
Contig	AY509120
Start / Stop / Direction	73,324 / 72,638 / - [in whole cluster] 73,324 / 72,638 / - [in contig]
Location	complement(72638..73324) [in whole cluster] complement(72638..73324) [in contig]
Type	CDS
Length	687 bp (228 aa)

Annotation

Category	5.1 general function
Product	putative two-component system response regulator
Product (GenBank)	putative two-component system response regulator
Gene
Gene (GenBank)	ORF8
EC number
Keyword
Note	It is supposed that this ORF is not involved in meilingmycin biosynthesis.
Note (GenBank)	similar to Streptomyces coelicolor SC3358, and Streptomyces avermitilis SAV4704
Reference	ACC Q5SFB8 PmId [15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004) comment Chalcomycin biosynthesis gene clusterに関する文献。 ORF8(228a.a.): Response regulator
Related Reference	ACC Q9ZEP4 PmId [10411727] A putative two-component signal transduction system regulates sigmaE, a sigma factor required for normal cell wall integrity in Streptomyces coelicolor A3(2). (Mol Microbiol. , 1999) comment 12th(Q9ZEP4) 60%, 3e-68 Streptomyces coelicolor_cseB Transcriptional regulatory protein CseB cseB null mutant作成あり。 CseB/CseC two-component systemはcell envelopeからのシグナルに応答してsigE promoterの活性を調節する。
Family/Domain	FD IPR001789, IPR001867 comment [IPR001789] Signal transduction response regulator, receiver domain (Domain) [IPR001867] Signal transduction response regulator, C-terminal (Domain)

Sequence

>putative two-component system response regulator [putative two-component system response regulator]
MTVLLVEDDEVIRRSVAMSLERYGYRVRVAADGLTGLELFREGRHDLVLLDVMLPGLDGI
GLCRRIRETATDPILMMSARGDALDVVSGLEAGADDYVVKPVDTAVLVARIRSLLRRAAF
VSPAPGPADPATPAGPLLFGDLSLDPAALEVRRGGERIALAPTELRLLLQFAAHPGIVLD
RQTLLREVWDYGWDGDTRVVDLCVQRLRKKIGAERVETVRGFGYKWKR

>putative two-component system response regulator [putative two-component system response regulator]
GTGACCGTCCTGCTGGTCGAGGACGACGAGGTGATCCGGCGGTCCGTCGCGATGTCCCTG
GAGCGCTACGGCTACCGCGTGCGCGTCGCGGCCGACGGACTGACCGGGCTCGAACTCTTC
CGGGAGGGACGGCACGACCTGGTGCTGCTCGACGTGATGCTGCCGGGCCTGGACGGCATC
GGACTGTGCCGGCGCATCCGGGAGACGGCCACGGACCCGATCCTGATGATGTCGGCGCGC
GGTGACGCCCTCGACGTGGTGTCCGGCCTGGAGGCCGGGGCCGACGACTACGTGGTGAAG
CCCGTCGACACCGCCGTCCTGGTGGCGCGCATCCGTTCGCTGCTGCGCCGGGCCGCCTTC
GTGTCCCCCGCCCCCGGACCCGCCGACCCCGCGACGCCGGCCGGCCCGCTGCTCTTCGGC
GACCTCTCCCTCGACCCCGCGGCCCTGGAGGTCCGCCGGGGCGGCGAGCGGATCGCGCTC
GCCCCGACCGAGCTGCGGCTGCTGCTCCAGTTCGCCGCGCACCCCGGGATCGTGCTGGAC
CGTCAGACCCTCCTGCGCGAGGTGTGGGACTACGGCTGGGACGGGGACACCCGGGTCGTC
GACCTGTGCGTGCAGCGGCTGCGCAAGAAGATCGGCGCCGAGCGCGTCGAGACGGTCCGG
GGCTTCGGCTACAAGTGGAAGCGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001789 Signal transduction response regulator, receiver domain (Domain) PF00072 Response_reg PS50110 RESPONSE_REGULATORY SM00448 REC IPR001867 Signal transduction response regulator, C-terminal (Domain) PF00486 Trans_reg_C SM00862 Trans_reg_C IPR011006 CheY-like superfamily (Domain) SSF52172 CheY_like IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd
SignalP	No significant hit
TMHMM	No significant hit

Chalc_00320 Chalc_00340