Chalc_00340 : CDS information

Location

Organism	Streptomyces bikiniensis
Strain	NRRL 2737
Entry name	Chalcomycin
Contig	AY509120
Start / Stop / Direction	75,081 / 73,651 / - [in whole cluster] 75,081 / 73,651 / - [in contig]
Location	complement(73651..75081) [in whole cluster] complement(73651..75081) [in contig]
Type	CDS
Length	1,431 bp (476 aa)

Annotation

Category	5.1 general function
Product	putative xanthine permease
Product (GenBank)	putative permease
Gene
Gene (GenBank)	ORF9
EC number
Keyword
Note	It is supposed that this ORF is not involved in chalcomycin biosynthesis.
Note (GenBank)	similar to SC6214, SC6212 from Streptomyces coelicolor; SAV2016, SAV1508, Streptomyces avermitilis, xanthine/uracil permease homology family member
Reference	ACC Q5SFB7 PmId [15561847] Chalcomycin biosynthesis gene cluster from Streptomyces bikiniensis: novel features of an unusual ketolide produced through expression of the chm polyketide synthase in Streptomyces fradiae. (Antimicrob Agents Chemother. , 2004) comment Chalcomycin biosynthesis gene clusterに関する文献。 ORF9(476a.a.): Permease
Related Reference	ACC P42086 PmId [9098051] Xanthine metabolism in Bacillus subtilis: characterization of the xpt-pbuX operon and evidence for purine- and nitrogen-controlled expression of genes involved in xanthine salvage and catabolism. (J Bacteriol. , 1997) comment 395th(P42086) 41%, 7e-84 B.subtilis_pbuX Xanthine permease Bacillus subtilisのXanthine metabolismについて。 Uptake rate of [14C]xanthineも見ている。pbuXの欠損株では取り込みが起こらなくなる。

Sequence

>putative xanthine permease [putative permease]
MPESRPAPEPTKTEDGTDARPDTPARPGDTHPVDQMLPPLKLFSAGLQHVAAMYAGVVAP
PLVVGIGVGLSTADIAFLMSASLFTSGIATLLQTLGFWKVGARLPFVNGVSFAGVAPMLA
IAKAEGPDDALPVIYGAVIVAGVFGFLLAPFFCKLIRFFPPVVTGTVITLIGVSLLPVAF
NWAQGGNAQAPDYGSLTYIGLATATLLITVVLRRVLTGFLKQISILLGLVAGTLLSLPLG
VADFSAVGDADVIGLPTPFHFGAPQFAAAAIISMCIVMLVSMTESTADVLALGEIVERPA
DEKTLAAALRADGLGTALSPLFNGFAASAFAQNVGLVAITKVRSRFVVAAAGGILLLLGL
CPLLASVVALIPQPVLGGVGIALFGTVAASGIQTLAGAALERGDNVLIVAISLGAGIIPI
AAPDFYHAFPEGARIVLDSGISTGCVVAVLLNLAFNHLGRRTDPAPETLPAPAAHH

>putative xanthine permease [putative permease]
ATGCCGGAATCCCGCCCCGCCCCGGAACCGACGAAGACCGAGGACGGAACCGACGCTCGT
CCCGACACCCCCGCAAGACCGGGCGACACCCATCCGGTCGACCAGATGCTGCCCCCGCTG
AAGCTCTTCAGCGCCGGTCTGCAGCACGTCGCCGCCATGTACGCCGGCGTCGTCGCGCCG
CCGCTGGTCGTCGGCATCGGCGTCGGCCTGTCCACCGCGGACATCGCGTTCCTCATGAGC
GCCAGCCTGTTCACCTCGGGCATCGCCACGCTCCTGCAGACGCTCGGCTTCTGGAAGGTC
GGAGCACGGCTCCCGTTCGTCAACGGCGTCTCCTTCGCCGGCGTCGCACCGATGCTCGCG
ATAGCGAAGGCCGAAGGCCCCGACGACGCGCTGCCCGTCATCTACGGCGCCGTGATCGTC
GCCGGCGTCTTCGGATTCCTCCTCGCCCCCTTCTTCTGCAAACTGATCCGCTTCTTCCCG
CCCGTGGTCACGGGCACGGTCATCACCCTCATCGGTGTCTCCCTGCTGCCCGTCGCGTTC
AACTGGGCCCAGGGCGGCAACGCGCAGGCACCGGACTACGGCAGCCTCACGTACATCGGC
CTCGCCACCGCCACCCTGCTGATCACCGTGGTCCTGCGCCGCGTCCTGACGGGCTTCCTC
AAGCAGATCTCCATCCTGCTGGGCCTCGTCGCCGGCACGCTCCTCTCGCTGCCGCTGGGC
GTCGCCGACTTCAGCGCCGTAGGCGACGCCGACGTCATCGGACTCCCCACCCCGTTCCAC
TTCGGCGCTCCGCAGTTCGCGGCCGCGGCGATCATCTCCATGTGCATCGTGATGCTCGTG
TCGATGACCGAGTCCACCGCCGACGTCCTCGCCCTCGGTGAGATCGTCGAGCGGCCGGCC
GACGAGAAGACCCTGGCCGCCGCGCTCCGCGCCGACGGCCTCGGCACCGCCCTCAGCCCG
CTCTTCAACGGCTTCGCCGCCAGCGCCTTCGCCCAGAACGTCGGCCTCGTCGCGATCACC
AAGGTCCGCAGCCGCTTCGTCGTAGCCGCGGCCGGCGGCATCCTCCTCCTGCTCGGCCTC
TGCCCGCTGCTCGCCTCCGTCGTCGCGCTCATCCCCCAGCCCGTCCTCGGCGGAGTGGGC
ATCGCCCTCTTCGGCACCGTCGCCGCCAGCGGCATCCAGACCCTCGCCGGAGCCGCCCTC
GAACGCGGCGACAACGTCCTCATCGTCGCCATCTCCCTGGGCGCCGGCATCATCCCCATC
GCCGCCCCGGACTTCTACCACGCCTTCCCCGAAGGCGCCCGCATCGTCCTCGACTCAGGC
ATCAGCACCGGATGTGTGGTCGCCGTCCTCCTCAACCTCGCCTTCAACCACCTCGGCCGC
AGGACCGACCCCGCTCCGGAGACGCTGCCCGCGCCTGCCGCACACCACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006042 Xanthine/uracil permease (Family) TIGR00801 Ncs2 PS01116 XANTH_URACIL_PERMASE IPR006043 Xanthine/uracil/vitamin C permease (Family) PF00860 Xan_ur_permease IPR017588 Xanthine permease (Family) TIGR03173 PbuX
SignalP	No significant hit
TMHMM	Transmembrane 11

Chalc_00330 Chalc_00350