Chth_00210 : CDS information

Location

Organism	Streptomyces antibioticus
Strain	DSM 40725
Entry name	Chlorothricin
Contig	DQ116941
Start / Stop / Direction	25,179 / 24,379 / - [in whole cluster] 25,179 / 24,379 / - [in contig]
Location	complement(24379..25179) [in whole cluster] complement(24379..25179) [in contig]
Type	CDS
Length	801 bp (266 aa)

Annotation

Category	1.4 Other
Product	putative type II thioesterase
Product (GenBank)	ChlK
Gene
Gene (GenBank)	chlK
EC number
Keyword	type II thioesterase
Note
Note (GenBank)	type II TE
Reference	ACC Q0R4N3 PmId [16793515] Genetic characterization of the chlorothricin gene cluster as a model for spirotetronate antibiotic biosynthesis. (Chem Biol. , 2006) comment chlorothricin(CHL) gene clusterに関する文献。 chlK(266 aa): typeII TE chlK自体の機能解析は行われておらず、文献中にも特に記載は無い。
Related Reference	ACC Q846W2 NITE Monen_00290 PmId [16984884] Identification of NanE as the thioesterase for polyether chain release in nanchangmycin biosynthesis. (Chem Biol. , 2006) comment 2nd, 63%, 3e-90 Streptomyces cinnamonensis_monAX Thioesterase [Monen_00290]type II thioesterase 対照実験として。 MonAXはdiketide-SNACを加水分解したが、polyether-SNACを加水分解しなかった。よって、polyether鎖の放出には関与しない。
Related Reference	ACC Q7BUF9 NITE Rifam_00540 PmId [19103602] Structure and functional analysis of RifR, the type II thioesterase from the rifamycin biosynthetic pathway. (J Biol Chem. , 2009) comment 19th, 52%, 4e-63 Amycolatopsis mediterranei_rifR RifR [Rifam_00540]type II thioesterase RifRは、acyl-CoAsよりacyl carrier domainのphosphopantetheinyl armからのacyl unitsをhydrolysisをする(Steady-state kinetics)。 helical lidの運動が、RifR active siteへの基質のアクセスを調節する(立体構造解析)。 RifRはCoA基質よりACP基質への反応を好む。これはtype II thioesteraseがcarrier domainsから異常unitsを除くようなhousekeeping functionsをすると考えられていることに一致する。

Sequence

>putative type II thioesterase [ChlK]
MSPTPDATDAWMRRIRPHADASVRLVCFPHAGGSATYYHPLAHSLARIPGAEVLAVQYPG
RQDRRREPFVDSVPLLADHVTAALNPLADRPLVLFGHSMGATLAYEVAHRLRARRPESEP
RWLLVSGRRAPSRFREGTVHLRTDAQLVEELRRVGGTDPAFLDDPELLDTILPVIRNDYR
AIESYRWTPAPPLTCPVTVLVGAQDPQTTLDEAAAWREHTSGPFALRDFPGGHFYLDVHR
QGVADALSQALERVARPMETLGGNGR

>putative type II thioesterase [ChlK]
TTGTCTCCTACTCCCGACGCGACGGATGCGTGGATGCGGCGCATCCGGCCCCACGCGGAC
GCTTCGGTCCGGCTCGTGTGTTTCCCGCACGCGGGTGGATCGGCCACCTACTACCACCCC
CTGGCCCACTCGCTCGCCCGGATACCCGGGGCCGAGGTGCTCGCGGTGCAGTACCCCGGC
CGGCAGGACCGGCGCAGGGAGCCATTCGTCGACAGTGTCCCCCTCCTCGCCGACCATGTC
ACCGCAGCCCTGAACCCGTTGGCCGACCGCCCGCTCGTCCTCTTCGGGCACAGCATGGGC
GCCACCCTCGCCTACGAGGTGGCCCACCGCCTCCGTGCGCGGCGGCCGGAGAGCGAACCG
AGGTGGCTGCTCGTCTCCGGGCGCCGGGCTCCGTCCCGCTTCCGCGAGGGGACGGTGCAT
CTGCGCACCGACGCCCAACTCGTCGAGGAACTGCGCCGCGTCGGCGGCACCGACCCCGCC
TTCCTCGACGACCCGGAGCTCCTGGACACGATCCTCCCGGTCATCCGCAACGACTACCGG
GCGATCGAGAGCTACCGCTGGACGCCGGCGCCACCCCTGACCTGCCCGGTCACCGTCTTG
GTCGGCGCCCAGGACCCGCAGACCACTCTCGACGAGGCGGCCGCGTGGCGCGAACACACC
AGCGGACCCTTCGCGTTGCGGGACTTCCCCGGTGGTCACTTCTACCTGGACGTCCACCGG
CAAGGCGTGGCCGACGCCCTCTCGCAGGCTCTGGAGCGGGTGGCCCGCCCGATGGAAACG
CTTGGAGGCAACGGACGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001031 Thioesterase (Domain) PF00975 Thioesterase
SignalP	No significant hit
TMHMM	No significant hit

Chth_00200 Chth_00220