Chth_00220 : CDS information

Location

Organism	Streptomyces antibioticus
Strain	DSM 40725
Entry name	Chlorothricin
Contig	DQ116941
Start / Stop / Direction	27,064 / 26,081 / - [in whole cluster] 27,064 / 26,081 / - [in contig]
Location	complement(26081..27064) [in whole cluster] complement(26081..27064) [in contig]
Type	CDS
Length	984 bp (327 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative dTDP-glucose 4,6-dehydratase
Product (GenBank)	ChlC2
Gene
Gene (GenBank)	chlC2
EC number	4.2.1.46
Keyword	D-olivose
Note
Note (GenBank)	dDNP-glucose-4,6-dehydratase
Reference	ACC Q0R4N2 PmId [16793515] Genetic characterization of the chlorothricin gene cluster as a model for spirotetronate antibiotic biosynthesis. (Chem Biol. , 2006) comment chlorothricin(CHL) gene clusterに関する文献。 chlC2(327 aa): dNDP-glucose-4,6-dehydratase chlC2自体の機能実験は行われていないが、相同性よりNGDH familyで、dNDP-4-keto-6-deoxyhexoseの形成に関与すると予測している。deoxysugar olivoseの生成に関与。
Related Reference	ACC Q9ZGH3 NITE Pikro_00130 PmId [9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998) [14570895] High resolution X-ray structure of dTDP-glucose 4,6-dehydratase from Streptomyces venezuelae. (J Biol Chem. , 2004) comment 46th, 64%, 1e-109 Streptomyces venezuelae_desIV [Pikro_00130]dTDP-glucose 4,6-dehydratase(EC 4.2.1.46) -- [PMID: 9770448] DesIV: dTDP-glucose 4,6-dehydratase desI, desII, desIII, desIV, desV, desVI, and desVIII はdeoxysugar生合成を担う。 --- [PMID: 14570895] DesIVの反応産物dTDP-4-keto-6-deoxy-D-glucoseを測定することで酵素活性をみて、activeなDesIV proteinのみを結晶化に使用している。
	ACC Q56173 NITE Grana_00110 PmId [7476861] Identification of Streptomyces violaceoruber Tu22 genes involved in the biosynthesis of granaticin. (Mol Gen Genet. , 1995) [9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998) comment 55th, 64%, 1e-108 Streptomyces violaceoruber_graE(gra-orf17) dTDP-glucose 4,6-dehydratase (EC 4.2.1.46) E.coliでORF2を発現させると、dTDP-glucose→dTDP-4-keto-6-deoxy-D-glucoseへの変換を触媒することができた(controlの5倍)。よって、ORF2はdTDP-glucose dehydrataseをコードする。 [PMID: 9831526]でrename ORF2→ORF17
	ACC Q194R3 NITE Mith_00070 PmId [9150235] Cloning and insertional inactivation of Streptomyces argillaceus genes involved in the earliest steps of biosynthesis of the sugar moieties of the antitumor polyketide mithramycin. (J Bacteriol. , 1997) comment 60th, 64%, 1e-107 Streptomyces argillaceus_mtmE dTDP-glucose 4,6-dehydratase Streptomyces argillaceusにおけるantitumor polyketide mithramycinのsugar moieties biosynthesis に関与するgenesについての文献。 mtmD, mtmEの二つの遺伝子をクローニングして、それぞれをE. coliで発現させて、それぞれがglucose-1-phosphate:dTTP thymidylyl transferaseとdTDP-D-glucose 4,6-dehydrataseであることを確認している。

Sequence

>putative dTDP-glucose 4,6-dehydratase [ChlC2]
MRYFVTGGAGFIGGHYVRALLAGAYPGTAGCEVTVLDKLTYAAHLDNLPLDHDRLTFVRG
DICDAGLLADLLPGHDAVVHFAAESHVDRSLADGTPFVTTNVLGTQILLDACARTGVPRF
VHVSTDEVYGSLRHGSWTEDTLLEPNSPYAASKAASDLMARAYWRTSDVAVSITRCSNNY
GTHQFIEKVVPLFVTNLLDGLDVPLYGDGHHVREWLHVEDHCRALHLVLTEGRAGEIYNI
GAGDGLTNRELTQRLLTLCGADWSRVREVPDRTAHDERYSVDTAKIREELGFTPRIGLDE
GLADVVAWYRDNRAWWEPLRERAALPA

>putative dTDP-glucose 4,6-dehydratase [ChlC2]
ATGAGGTACTTCGTCACCGGCGGCGCCGGATTCATCGGCGGCCACTATGTGCGCGCCCTG
CTCGCCGGCGCCTACCCGGGCACCGCCGGCTGCGAGGTCACCGTCCTGGACAAGCTGACC
TACGCGGCCCACCTCGACAACCTCCCCCTGGACCACGACCGGTTGACCTTCGTACGCGGC
GACATCTGCGACGCCGGCCTGCTGGCGGACCTGCTGCCCGGACATGACGCCGTGGTGCAC
TTCGCCGCCGAGTCGCACGTCGACCGCTCCCTCGCCGACGGCACGCCCTTCGTGACGACG
AACGTGCTCGGCACCCAGATCCTCCTCGACGCCTGCGCCCGCACGGGCGTTCCGCGGTTC
GTCCATGTCTCCACCGACGAGGTCTACGGCTCACTGCGGCACGGCTCCTGGACCGAGGAC
ACCCTCCTGGAGCCCAACTCCCCGTACGCGGCCTCGAAGGCGGCGAGCGATCTGATGGCC
CGCGCCTACTGGCGCACCAGCGATGTCGCCGTCTCCATCACACGGTGCTCCAACAACTAC
GGCACCCACCAGTTCATCGAGAAGGTCGTACCGCTGTTCGTGACCAACCTGCTGGACGGC
CTGGACGTGCCGCTGTACGGGGACGGTCACCATGTGCGCGAGTGGCTGCATGTCGAGGAC
CACTGCCGCGCCCTCCACCTGGTGCTCACCGAGGGGCGCGCCGGCGAGATCTACAACATC
GGTGCCGGAGACGGGCTGACCAACCGGGAACTGACGCAGCGTCTGCTCACGCTCTGCGGG
GCGGACTGGAGCCGGGTGCGCGAGGTTCCCGACCGGACGGCCCACGACGAGCGGTACTCG
GTCGACACGGCGAAGATCCGCGAGGAGCTGGGCTTCACACCGAGGATCGGGCTGGACGAG
GGACTGGCGGACGTCGTGGCCTGGTACCGCGACAACCGCGCGTGGTGGGAGCCGCTGCGC
GAGCGGGCGGCCCTGCCCGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR005888 dTDP-glucose 4,6-dehydratase (Family) TIGR01181 DTDP_gluc_dehyt IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	Eukaryota
TMHMM	No significant hit

Chth_00210 Chth_00230