Chro_00050 Chro_00050   Chro_00070 Chro_00070

Chro_00060 : CDS information

close this sectionLocation

Organism
StrainATCC 13273 (=NBRC 3746)
Entry nameChromomycin
Contig
Start / Stop / Direction4,438 / 5,193 / + [in whole cluster]
4,438 / 5,193 / + [in contig]
Location4438..5193 [in whole cluster]
4438..5193 [in contig]
TypeCDS
Length756 bp (251 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose C-4 ketoreductase
Product (GenBank)NDP-4-ketoreductase
Gene
Gene (GenBank)cmmUII
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004)
comment
aureolic acid type antitumor drug chromomycin A3の生合成gene clusterの同定論文。

CmmUII : NDP-4-ketoreductase

配列解析から機能推定。
CmmUI と CmmUII はurdamycin D-olivose生合成に関連するUrdRに似ている。
よってchromomycinに含まれるdeoxysugarのうち、D-deoxysugarsのD-olivose and D-oliose生合成に関連すると提唱されている。
D-olivose と D-olioseの違いはこのketoreductaseによってもたらされるであろうC-4 OH基の立体配置。
Related Reference
ACC
Q9L4S5
NITE
Urd_00250
PmId
[11094336] The NDP-sugar co-substrate concentration and the enzyme expression level influence the substrate specificity of glycosyltransferases: cloning and characterization of deoxysugar biosynthetic genes of the urdamycin biosynthetic gene cluster. (Chem Biol. , 2000)
comment
Blast 2nd, id52%, 8e-65
Streptomyces fradiae_urdR
NDP-hexose 4-ketoreductase UrdR

urdamycinは、aglycone + NDP-D-olivose, NDP-L-rhodinose修飾によってA,B,G,M,N,Oと名づけられている。これに含まれるNDP-D-olivose,NDP-L- rhodinose合成系(D-Glc-1-P=>=>)に関与する遺伝子群の実験(gene cloning,mutant作製)。

このurdRは、NDP-D-olivose合成系に関与。
NDP-4-keto-2,6-dideoxy-D-Glc=>NDP-D-olivose
urdR mutantがurdamycin M(D-rhodinose含)蓄積することと相同性より機能推測している。

close this sectionSequence

selected fasta
>putative NDP-hexose C-4 ketoreductase [NDP-4-ketoreductase]
MRIIGHGFLARSIRPLASSHPRVVAFAAGVSSGDGVAEDQFAREAALLYDTLGQCLRDGL
RLVYFSTSSAGMYGPRHGAGREDGPVRPFSSYGRHKLAMEEVIRNSGADHLILRLAYPVG
PGQRPHQFLPSLVAQVRRGRVRVHRGARRDLIDVRHAVSLLKSLLHEDVSRQVVNIASGW
EAAVEDIVGHLEDRTGIVAERHTVDVPAEGPVSTERLHRLVPKAAELGFGPCYYRQVIDS
YLAAVAPSSPA
selected fasta
>putative NDP-hexose C-4 ketoreductase [NDP-4-ketoreductase]
ATGCGCATCATCGGCCACGGTTTCCTGGCGCGCAGCATCCGTCCGCTCGCCAGCTCACAC
CCACGTGTCGTCGCCTTCGCGGCCGGCGTGTCCAGCGGCGACGGCGTCGCCGAGGACCAG
TTCGCACGTGAGGCCGCCCTGTTGTACGACACGCTTGGCCAGTGCCTGCGCGACGGCCTG
CGGCTCGTGTACTTCTCGACCTCGTCCGCCGGGATGTACGGGCCACGGCACGGTGCCGGG
CGGGAGGACGGCCCAGTACGCCCCTTCTCTTCTTACGGCAGACACAAACTGGCCATGGAG
GAGGTCATCAGGAACTCTGGCGCGGACCACCTGATCCTGCGTCTCGCCTATCCGGTCGGC
CCGGGGCAGCGCCCACACCAGTTCCTGCCCTCGCTGGTCGCCCAGGTCCGGCGGGGCCGC
GTCCGGGTCCACCGGGGAGCGCGTCGCGATCTGATCGACGTCCGCCATGCCGTGTCCCTG
CTCAAGAGCCTCCTCCATGAAGACGTCTCGCGGCAGGTGGTCAACATCGCCTCCGGCTGG
GAGGCGGCCGTGGAGGACATCGTCGGCCACCTCGAAGACCGCACCGGCATCGTGGCCGAG
CGCCACACCGTCGACGTACCCGCGGAGGGGCCCGTATCCACCGAGCGGCTGCACAGGCTC
GTCCCGAAGGCGGCGGAGCTGGGCTTCGGACCCTGCTACTACCGCCAGGTCATCGACTCC
TATCTGGCAGCCGTCGCGCCTTCGAGTCCGGCTTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001509 NAD-dependent epimerase/dehydratase (Domain)
[18-177] 8.40000000000001e-14 PF01370
PF01370   Epimerase
IPR016040 NAD(P)-binding domain (Domain)
[12-192] 8.1e-18 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-27] 0.593 Signal
Bacteria, Gram-positive   
[1-35] 0.59 Signal
Eukaryota   
TMHMM No significant hit
Chro_00050 Chro_00050   Chro_00070 Chro_00070
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