Chro_00040 Chro_00040   Chro_00060 Chro_00060

Chro_00050 : CDS information

close this sectionLocation

Organism
StrainATCC 13273 (=NBRC 3746)
Entry nameChromomycin
Contig
Start / Stop / Direction3,417 / 4,427 / + [in whole cluster]
3,417 / 4,427 / + [in contig]
Location3417..4427 [in whole cluster]
3417..4427 [in contig]
TypeCDS
Length1,011 bp (336 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose C-3 ketoreductase
Product (GenBank)NDP-3-ketoreductase
Gene
Gene (GenBank)cmmW
EC number
Keyword
  • D-olivose
  • D-oliose
  • L-chromose
Note
Note (GenBank)
Reference
ACC
PmId
[15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004)
comment
aureolic acid type antitumor drug chromomycin A3の生合成gene clusterの同定論文。

CmmW: NDP-3-ketoreductase

配列解析から機能推定。CmmWは、granaticinのD-olivose生合成においてequatorialなC-3 OH基を与える3-ketoreductaseに似ている。
すべてのchromomycin deoxysugars生合成において、CmmVによる2,3-dehydrationと共に働いてC-2 deoxygenationし、NDP-4-keto-2,6-dideoxy-D-glucoseをもたらすと提唱されている。
Related Reference
ACC
Q9ALN5
NITE
Spino_00080
PmId
[18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008)
comment
Blast 14th, id49%, 5e-75
Saccharopolyspora spinosa_spnN
Probable NDP-hexose-3-ketoreductase

Forosamine合成過程で、3位のケト基の還元に関与する。

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NAD(P)H-dependent dTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductase_SpnN

dTDP-D-glucose, RfbB(dTDP-D-glucose → dTDP-4-keto-6-deoxy-D-glucose), TylX3(SpnNの1つ前の反応を担うSpnOのhomologue), SpnN, and NADPHをインキュベーションしてできた産物がdTDP-4-keto-2,6-dideoxy-D-glucoseであることをHPLC chromatogram、1H NMR spectrumなどで確認している。NADPHはNADHの2倍速く消費されるので、おそらくNADPH-dependentである。

close this sectionSequence

selected fasta
>putative NDP-hexose C-3 ketoreductase [NDP-3-ketoreductase]
MGPRLRVGVLGCASIARRKMLPALASNPSVRLVAVASRTGEKATMFAAPYGCDAVTGYEE
LLQRTDVDAVYIPLPAALRATWIARALEAGKHVLGEKPLTTTAADARDLVALGGRRGLLL
MESFMFLHHSVHRRVTELVAAGRIGEPRSFTAEFTIPPLPADDIRNRPGLGGGALLDLGV
YTVRAAQYFCGPGLSVAGAVLMSDPPVAGAPPATGRGVDRAGAALLTSDRTTAQLTFGIA
HAYQCRYRICGSEGLLSLDRAFTPPPNLRPVVRIEDKDGTEERALEADDHFANIVTAFAG
TALGGGDLTGHGEQILRQADLVERIRRAGAPAPRAA
selected fasta
>putative NDP-hexose C-3 ketoreductase [NDP-3-ketoreductase]
GTGGGCCCGAGGCTGAGGGTCGGCGTACTCGGCTGCGCCTCCATCGCCCGCCGCAAGATG
CTGCCCGCCCTGGCGTCGAACCCGTCGGTCCGACTGGTGGCGGTGGCCAGCCGCACCGGC
GAGAAGGCGACCATGTTCGCGGCTCCCTACGGGTGCGATGCGGTCACGGGCTACGAGGAA
CTGCTGCAGCGCACCGACGTCGACGCCGTGTACATCCCGCTGCCCGCCGCACTGCGGGCC
ACCTGGATCGCGCGCGCTCTGGAAGCCGGCAAACACGTCCTCGGAGAGAAACCCCTCACC
ACGACCGCCGCCGACGCCCGGGACTTGGTGGCCCTCGGCGGACGGCGGGGACTGCTGCTG
ATGGAGAGCTTCATGTTCCTCCACCACTCCGTCCACCGGCGCGTCACCGAACTCGTCGCC
GCCGGACGCATCGGCGAACCACGCTCGTTCACCGCCGAGTTCACCATCCCGCCGCTTCCC
GCCGATGACATCCGCAACCGTCCTGGCCTCGGCGGCGGCGCCCTGCTCGACCTGGGCGTC
TACACCGTCCGGGCGGCGCAGTACTTCTGCGGCCCCGGCCTGTCCGTGGCGGGCGCGGTG
CTCATGAGCGACCCGCCCGTGGCAGGCGCGCCCCCGGCCACCGGCCGCGGGGTCGACCGG
GCGGGGGCGGCGCTGCTGACCTCGGACCGGACCACCGCCCAGCTGACCTTCGGCATAGCC
CACGCCTACCAGTGCCGTTACCGCATCTGCGGCAGCGAGGGCCTCCTCTCACTGGACCGG
GCGTTCACCCCGCCGCCCAACCTGCGGCCCGTGGTGCGCATCGAGGACAAGGACGGCACC
GAGGAACGGGCACTCGAGGCCGACGACCACTTCGCCAACATCGTCACGGCCTTCGCCGGC
ACCGCCCTGGGCGGCGGTGACCTCACCGGGCATGGGGAGCAGATCCTCCGCCAGGCCGAC
CTGGTGGAGCGCATCCGACGGGCGGGTGCTCCCGCCCCCCGCGCCGCATAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000683 Oxidoreductase, N-terminal (Domain)
[5-121] 9.5e-25 PF01408
PF01408   GFO_IDH_MocA
IPR004104 Oxidoreductase, C-terminal (Domain)
[137-249] 1.4e-08 PF02894
PF02894   GFO_IDH_MocA_C
IPR016040 NAD(P)-binding domain (Domain)
[3-146] 1.80000000000002e-43 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-25] 0.509 Signal
Bacteria, Gram-negative   
[1-36] 0.605 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Chro_00040 Chro_00040   Chro_00060 Chro_00060
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