Chro_00040 : CDS information

Location

Organism	Streptomyces griseus
Strain	ATCC 13273 (=NBRC 3746)
Entry name	Chromomycin
Contig	AJ578458
Start / Stop / Direction	2,050 / 3,432 / + [in whole cluster] 2,050 / 3,432 / + [in contig]
Location	2050..3432 [in whole cluster] 2050..3432 [in contig]
Type	CDS
Length	1,383 bp (460 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose 2,3-dehydratase
Product (GenBank)	NDP-2,3-dehydratase
Gene
Gene (GenBank)	cmmV
EC number
Keyword	D-olivose D-oliose L-chromose
Note
Note (GenBank)
Reference	ACC Q70J94 PmId [15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004) comment aureolic acid type antitumor drug chromomycin A3の生合成gene clusterの同定論文。 CmmV: NDP-2,3-dehydratase 配列解析から機能推定。抗生物質生合成におけるC-2 deoxygenation stepに関連したdTDP-2,3-glucose dehydrataseにとても似ている。すべてのchromomycin deoxysugars生合成において、CmmWによる3-reductionと共に働き、C-2 deoxygenationすると提唱されている。
Related Reference	ACC Q9ALN6 NITE Spino_00070 PmId [18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008) comment Blast 28th, id48%, 1e-108 Saccharopolyspora spinosa_spnO Probable NDP-hexose-2,3-dehydratase Forosamine合成の初期段階で NDP-4-keto-6-deoxy-glucoseの2,3位の脱水反応を行う。 dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase_SpnO dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), and either NADH or NADPHと一緒にSpnO and SpnNをインキュベートし、NAD(P)Hの消費量をフォローしてtandem reactionを測定。SpnO or SpnNのどちらかを欠いた場合、消費が見られなかった。 dTDP-3,4-diketo-2,6-dideoxy-D-glucoseは不安定で急速にdTDP and maltolに分解してしまうため、SpnOのみの触媒効率は定量できていない。 -- SpnN はdTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductaseであることが他で報告されている。

Sequence

>putative NDP-hexose 2,3-dehydratase [NDP-2,3-dehydratase]
MAQAATRTARDRAVHRYGPRLAHSAQAGDRAGGIPGWLDERRRSGTFEVRRIPFGEMVKW
RIDPDTGNLVHDSGRFFSIEGLTVRADSGTTWSQPIINQPETGVLGILVKEIDGVLRFLM
QAKMEPGNIGLFQLGPTVQATRSNYTGVHRGRPTPYIEYFVGERRGEVLTDVLQSEQGDS
FLRKRNRNMVVEITEDIEVRDDYRWLTLGEIHQLLAVDNLVNMDARTVLSSLPFPPGDED
AAPHSDTEILSWLTGIKTRHEREVVTSPLARIEDWRRTPAEIARSDGRHLRVLAVSVQAG
NREVTNWTQPLLAAQRQGIAAFIVRRTAGEPQVLVQGLTQPGTFDVAEMAPTVQCVTDGW
HDLPEKPPYLDYVLAVPDDRVHYSALLSEEGGRFYRRDNRYLVVEADETLGGDPPDAYRW
LGFSQLKRFTGYSNHVDVEARTLIACLQSLWGKEPLWARG

>putative NDP-hexose 2,3-dehydratase [NDP-2,3-dehydratase]
ATGGCTCAGGCAGCGACACGGACGGCCCGGGACCGGGCCGTCCACCGGTACGGTCCCCGG
CTGGCCCATTCGGCCCAGGCCGGCGACCGAGCCGGCGGGATACCCGGCTGGCTCGACGAA
CGCCGGCGGTCCGGCACGTTCGAGGTCCGGCGCATCCCCTTCGGCGAGATGGTGAAATGG
CGGATCGACCCGGACACCGGGAACCTGGTGCACGACAGCGGACGCTTCTTCTCGATCGAG
GGCCTGACGGTCCGTGCGGACTCCGGCACCACATGGTCCCAGCCGATCATCAACCAGCCC
GAGACCGGGGTGCTCGGCATCCTCGTCAAGGAGATCGACGGTGTCCTGCGGTTCCTGATG
CAGGCCAAGATGGAGCCCGGGAACATCGGCCTGTTCCAGCTCGGCCCCACCGTCCAGGCC
ACGCGCAGCAACTACACCGGAGTGCACCGGGGGCGGCCCACCCCCTACATCGAGTACTTC
GTGGGGGAGCGGCGCGGCGAGGTGCTCACGGACGTGCTCCAGTCCGAACAGGGCGACAGC
TTCCTGCGCAAGCGCAACCGCAACATGGTCGTGGAGATCACCGAGGACATCGAGGTCCGC
GACGACTACCGCTGGCTCACCCTGGGCGAGATCCATCAGCTCCTCGCGGTGGACAACCTC
GTCAACATGGACGCCCGCACGGTTCTGTCGTCGCTGCCGTTCCCCCCGGGGGACGAGGAC
GCGGCTCCGCACAGCGACACCGAGATCCTGAGCTGGCTCACCGGGATCAAGACCCGCCAC
GAACGGGAGGTCGTCACCTCGCCGCTCGCCCGGATCGAGGACTGGCGGCGCACACCGGCG
GAGATCGCCCGCAGCGACGGCCGCCACCTGCGGGTGCTCGCCGTCTCCGTACAGGCGGGA
AACCGCGAGGTGACCAACTGGACACAGCCGTTGCTGGCCGCCCAGCGCCAGGGCATTGCC
GCATTCATCGTCCGGCGTACCGCGGGCGAGCCGCAGGTCCTCGTCCAGGGACTCACCCAG
CCCGGCACCTTCGACGTCGCCGAGATGGCGCCGACCGTGCAGTGCGTCACGGACGGCTGG
CACGATCTGCCGGAGAAGCCGCCCTACCTGGACTACGTGCTCGCCGTACCCGACGACCGT
GTGCACTACTCGGCGCTGCTGTCCGAGGAGGGCGGCAGGTTCTACCGGCGCGACAACCGC
TATCTCGTCGTCGAGGCCGACGAGACCCTCGGCGGTGACCCGCCCGACGCCTACCGGTGG
CTGGGGTTCTCCCAGCTCAAGCGGTTCACCGGTTACAGCAATCACGTGGACGTGGAGGCG
CGCACCCTGATCGCCTGCCTCCAGTCCCTCTGGGGCAAGGAGCCGCTGTGGGCCCGAGGC
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005212 NDP-hexose 2,3-dehydratase (Domain) PF03559 Hexose_dehydrat
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Chro_00030 Chro_00050