Chro_00020 Chro_00020   Chro_00040 Chro_00040

Chro_00030 : CDS information

close this sectionLocation

Organism
StrainATCC 13273 (=NBRC 3746)
Entry nameChromomycin
Contig
Start / Stop / Direction1,836 / 1,585 / - [in whole cluster]
1,836 / 1,585 / - [in contig]
Locationcomplement(1585..1836) [in whole cluster]
complement(1585..1836) [in contig]
TypeCDS
Length252 bp (83 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase acyl carrier protein subunit
Product (GenBank)acyl carrier protein
Gene
Gene (GenBank)cmmS
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
Reference
ACC
PmId
[15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004)
comment
aureolic acid type antitumor drug chromomycin A3の生合成gene clusterの同定論文。

CmmS: ACP

配列解析から機能推定。
minimal PKSのACP subunitであると提唱されている。
KS, CLFから8kbp以上離れた下流にある。
Related Reference
ACC
Q56165
NITE
Jado_00100
PmId
[7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994)
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
Blast 5th, id55%, 1e-16
Streptomyces venezuelae_acp(jadC)
Acyl carrier protein

--
[PMID:7881555](1994)
ORF3(=JadC)は配列解析からtype II PKSのACPであると提唱されている。
ORF2-ORF4領域を含んだplasmidの染色体DNAへの組み込みは、jadomycin B産生を激しく抑制したが、成長や胞子色素沈着には影響なし。

--
[PMID: 9224566](1997)
minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、そのplasmid発現株での産物を解析。
jadCもACPとして使用されている。

close this sectionPKS/NRPS Module

ACP3..76

close this sectionSequence

selected fasta
>polyketide synthase acyl carrier protein subunit [acyl carrier protein]
MMTAEDLKRIMREGAGQDEGVDLDGDIADVPFTDLGYDSLALLETVSRVEREFGGPIADE
IVGAAKTPGEFLHLVNLGMSGAS
selected fasta
>polyketide synthase acyl carrier protein subunit [acyl carrier protein]
ATGATGACTGCGGAAGACCTGAAGAGGATCATGCGTGAGGGGGCTGGCCAGGACGAGGGC
GTCGATCTGGACGGTGACATCGCCGACGTGCCCTTCACCGACCTCGGGTACGACTCGCTC
GCCTTGCTGGAGACGGTGAGCAGGGTGGAGCGCGAGTTCGGGGGGCCGATCGCCGACGAG
ATCGTCGGCGCCGCGAAGACACCGGGCGAGTTCCTGCACCTCGTCAACCTGGGCATGTCG
GGGGCGAGCTGA
ACP3..76
ACP7..228

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006162 Phosphopantetheine attachment site (PTM)
[34-49] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[2-75] 4.99999811956429e-12 SSF47336
SSF47336   ACP_like
[3-76] PS50075
PS50075   ACP_DOMAIN
[27-74] 4.5e-07 PF00550
PF00550   PP-binding
[3-75] 7.5e-13 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
SignalP No significant hit
TMHMM No significant hit
Chro_00020 Chro_00020   Chro_00040 Chro_00040
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