Chro_00010 Chro_00010   Chro_00030 Chro_00030

Chro_00020 : CDS information

close this sectionLocation

Organism
StrainATCC 13273 (=NBRC 3746)
Entry nameChromomycin
Contig
Start / Stop / Direction1,583 / 825 / - [in whole cluster]
1,583 / 825 / - [in contig]
Locationcomplement(825..1583) [in whole cluster]
complement(825..1583) [in contig]
TypeCDS
Length759 bp (252 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative oxidoreductase
Product (GenBank)ketoreductase
Gene
Gene (GenBank)cmmTI
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004)
comment
aureolic acid type antitumor drug chromomycin A3の生合成gene clusterの同定論文。

CmmTI : Ketoreductase

配列解析から機能推定。
chromomycin生合成において割り当てられる役割なし。
MtmTI に似ている。mithramycin生合成におけるその機能ははっきりと確立されていないが、MtmXと共に環化に関係することが提唱されている。
Related Reference
ACC
P95727
PmId
[1597409] Purification and characterization of a novel enoyl coenzyme A reductase from Streptomyces collinus. (J Bacteriol. , 1992)
[8955309] Cloning and characterization of the gene encoding 1-cyclohexenylcarbonyl coenzyme A reductase from Streptomyces collinus. (J Bacteriol. , 1996)
[10973220] Identification of a cyclohexylcarbonyl CoA biosynthetic gene cluster and application in the production of doramectin. (Nat Biotechnol. , 2000)
comment
Blast 46th, id35%, 3e-27
Streptomyces collinus_chcA
1-cyclohexenylcarbonyl CoA reductase

---
[PMID: 1597409](1992)
酵素を分離して活性測定。

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[PMID: 8955309](1996)
E.coliで異種性発現したS. collinus chcAを分離して活性測定。
vitroでcyclohexanecarboxylic acidの形成に関与する3つの還元ステップを触媒する。
S. collinus chcA deletion mutantは、ChcA activityと、cyclohexanecarboxylic acid または ansatrieninを合成する能力を失った。

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[PMID: 10973220](2000) abstract
S. collinusのansatrienin生合成gene clusterにおける5つのputative CHC-CoA生合成遺伝子を同定。

close this sectionSequence

selected fasta
>putative oxidoreductase [ketoreductase]
MRPGGPAEGKVALVAGGTRGTGFAVARTLMRRGCHVFLNYAHDEGGAKEAVTALRGEGLP
GTATALRSDITRPDALPRLLHGIRQHHDRLDFFVHSAVSFHPMDAADPAPEAMATDLTAA
LAPLVYGVRPLVRAMAGGPGRVVVVSSTGAQRVVPRYVGAGIAKAALEAQVRYLAVELAP
AGITVNGVSTAKLDKGRAGDPALMRALAARTPAGRLTTPDDIAAVVALLCSDDARWIHGQ
VITADGGLTLRA
selected fasta
>putative oxidoreductase [ketoreductase]
GTGCGGCCGGGTGGGCCCGCCGAGGGCAAGGTCGCGCTCGTGGCCGGCGGTACCCGGGGC
ACAGGGTTCGCCGTCGCCCGTACGCTGATGCGCCGCGGCTGCCACGTGTTCCTCAACTAC
GCGCACGACGAGGGCGGGGCGAAGGAAGCGGTGACCGCGCTCCGGGGTGAGGGGCTGCCC
GGGACCGCCACGGCGCTCCGGAGCGACATCACCCGGCCCGACGCCCTGCCCCGGCTGCTG
CACGGGATCCGGCAGCACCACGACCGCCTCGACTTCTTCGTGCACAGCGCCGTCTCGTTC
CATCCGATGGACGCGGCGGATCCGGCGCCCGAGGCCATGGCCACGGACCTGACGGCGGCG
CTGGCCCCTCTGGTGTACGGCGTCCGGCCGCTCGTCCGGGCCATGGCGGGCGGGCCAGGG
CGCGTGGTGGTCGTGTCCAGCACCGGCGCCCAGCGCGTGGTCCCCCGTTACGTCGGCGCC
GGGATCGCCAAGGCCGCGCTGGAGGCTCAGGTCCGCTATCTGGCCGTGGAGCTCGCCCCC
GCGGGTATCACCGTCAACGGCGTGTCCACCGCGAAACTCGACAAGGGCCGCGCAGGCGAC
CCCGCCCTGATGCGGGCGCTCGCCGCGCGGACCCCGGCGGGACGGCTGACCACCCCGGAC
GACATAGCCGCCGTGGTGGCGTTGCTCTGCTCCGACGACGCCCGGTGGATCCACGGCCAG
GTGATCACGGCCGACGGCGGTCTGACTCTGCGCGCGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002347 Glucose/ribitol dehydrogenase (Family)
[11-28] 1.39999892049878e-21 PR00081 [134-150] 1.39999892049878e-21 PR00081 [160-179] 1.39999892049878e-21 PR00081 [181-198] 1.39999892049878e-21 PR00081 [212-232] 1.39999892049878e-21 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[8-250] 2.50000000000001e-57 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-41] 0.146 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Chro_00010 Chro_00010   Chro_00030 Chro_00030
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