Chro_00090 Chro_00090   Chro_00110 Chro_00110

Chro_00100 : CDS information

close this sectionLocation

Organism
StrainATCC 13273 (=NBRC 3746)
Entry nameChromomycin
Contig
Start / Stop / Direction11,224 / 9,956 / - [in whole cluster]
11,224 / 9,956 / - [in contig]
Locationcomplement(9956..11224) [in whole cluster]
complement(9956..11224) [in contig]
TypeCDS
Length1,269 bp (422 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase beta-ketoacyl synthase subunit
Product (GenBank)ketosynthase
Gene
Gene (GenBank)cmmP
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
Reference
ACC
PmId
[15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004)
comment
aureolic acid type antitumor drug chromomycin A3の生合成gene clusterの同定論文。

CmmP: KS alpha

配列解析から機能推定。
minimal PKSのKS alpha subunitであると提唱されている。
Related Reference
ACC
Q54495
NITE
Nogl_00370
PmId
[8668120] A gene cluster involved in nogalamycin biosynthesis from Streptomyces nogalater: sequence analysis and complementation of early-block mutations in the anthracycline pathway. (Mol Gen Genet. , 1996)
comment
Blast 3rd, id75%, 0.0
Streptomyces nogalater_snoa1
Snoa1
[DoBISCUIT]polyketide synthase beta-ketoacyl synthase subunit

S. nogalaterのanthracycline生合成gene clusterを解析。
11kb領域に11ORFs(snoEDCBA123XYZ)が同定されている。

sno1: KS, AT

aclacinomycin生合成がブロックされたS.galilaeus mutantsの相補によりminimal PKSを確認している。
ACC
Q54173
NITE
Urd_00120
PmId
[7592377] Cloning and characterization of a polyketide synthase gene from Streptomyces fradiae Tu2717, which carries the genes for biosynthesis of the angucycline antibiotic urdamycin A and a gene probably involved in its oxygenation. (J Bacteriol. , 1995)
comment
Blast 4th, id76%, 1e-180
Streptomyces fradiae_urdA
Putative ketoacyl synthase

urdEFABCDを含むcosmid purd8をS.lividans TK24へ導入すると、urdamycin抵抗性を獲得。
urdamycin産生株S.fradiae Tu2717染色体から、urdE-C末+urdFABを含むfragment g領域を削除するとurdamycin産生不可。

AA配列解析で、UrdAはPKS gene clustersのbeta-ketoacyl synthasesに高い類似性あり。
UrdAとUrdBはid36%で似ているが、UrdBにはないactive site cysteineをUrdAは持っている。

TcmK ketoacyl synthase or TcmL chain length factorのどちらかを障害してtetracenomycin C非産生にしたStreptomyces glaucescensのmutantで、urdFABを異種性発現したらどちらのmutantも相補され、tetracenomycin Cを産生できた。

close this sectionPKS/NRPS Module

KS3..375

close this sectionSequence

selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [ketosynthase]
MNRRVVLTGIGVVAPGGTGTKAFWELITSGGTATRPISLFDASPFRSRIAAECDFDPAAH
GLTHKETRRMDRAAQFAAVATDEAVADAGLEFHRTDPARVGVTVGSAVGCTTSLEREYTA
VSDGGRDWLVDHTYAVPHLYDHLVPSSMAAEVAWRVRAEGPASVVSTGCTSGLDAVAHAR
GLILEGSADVMVAGATDAPISPITLACFDAIKATTPRNDDPEHASRPFDATRNGFVLGEG
SAMFVLEEYEHAVRRGARPYAEITGFASRCNAFHMTGLRPDGREMAEAIGRALAEAGARP
DDIDYVNAHGSGTKQNDRHETAAFKAALGEHAYAVPVSSIKSMVGHSLGAIGAIEIAACA
LAVRHGVVPPTANLHEPDPECDLDYVPLRARHRALDRVLTVGSGFGGFQSAMVLAAPEER
AS
selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [ketosynthase]
ATGAACAGGCGTGTCGTCCTCACGGGAATCGGTGTGGTGGCCCCCGGCGGCACGGGCACC
AAGGCGTTCTGGGAGCTGATCACCTCCGGGGGCACGGCGACCCGGCCGATCTCCCTCTTC
GACGCCTCCCCGTTCCGGTCGCGGATCGCCGCCGAGTGCGACTTCGACCCCGCCGCGCAC
GGACTGACCCACAAGGAGACCCGGCGGATGGACCGAGCCGCCCAGTTCGCAGCGGTCGCC
ACCGACGAGGCCGTCGCCGACGCCGGACTGGAGTTCCACCGCACCGACCCCGCACGGGTG
GGAGTCACGGTCGGCAGCGCGGTCGGCTGCACCACCAGCCTGGAACGCGAGTACACCGCG
GTCAGCGACGGCGGCCGGGACTGGCTCGTCGACCACACCTACGCCGTACCCCACCTCTAC
GACCACCTCGTCCCGAGCTCGATGGCCGCCGAAGTGGCCTGGCGGGTACGTGCCGAGGGC
CCCGCCTCGGTCGTCTCGACCGGCTGCACCTCCGGGCTCGACGCGGTCGCCCACGCCCGC
GGCCTGATCCTGGAGGGCAGCGCCGACGTGATGGTGGCCGGCGCGACCGACGCGCCGATC
TCCCCGATCACCCTGGCCTGCTTCGACGCCATCAAGGCGACCACCCCGCGCAACGACGAC
CCGGAGCACGCCTCACGTCCCTTCGACGCCACCCGGAACGGCTTCGTGCTGGGCGAGGGA
TCCGCGATGTTCGTCCTGGAGGAGTACGAGCACGCGGTACGCCGAGGGGCGCGCCCCTAC
GCCGAGATCACCGGCTTCGCCTCCCGCTGCAACGCCTTCCACATGACGGGCCTGCGCCCG
GACGGCAGGGAGATGGCCGAGGCCATCGGGCGGGCGCTCGCCGAGGCGGGGGCCCGCCCC
GACGACATCGACTACGTGAACGCGCACGGCTCGGGCACCAAGCAGAACGACCGGCACGAG
ACGGCCGCCTTCAAGGCCGCGCTCGGCGAGCACGCGTACGCCGTCCCCGTCAGTTCCATC
AAGTCGATGGTGGGGCACTCGCTCGGGGCGATCGGCGCGATCGAGATCGCCGCCTGTGCG
CTGGCCGTCCGGCACGGCGTCGTCCCGCCGACCGCCAATCTGCACGAGCCCGACCCCGAG
TGCGACCTGGACTACGTGCCGCTGCGCGCCCGGCACCGGGCGCTGGACAGGGTGCTGACC
GTGGGCAGCGGATTCGGCGGCTTCCAGAGCGCCATGGTGCTCGCCGCCCCCGAGGAGCGT
GCGTCATGA
KS3..375
KS7..1125

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[3-251] 3.40000000000004e-60 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[260-375] 6.40000000000004e-40 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[2-265] 3.79999999999991e-65 G3DSA:3.40.47.10 [270-418] 4.6e-55 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[2-419] 7.80005155399507e-70 SSF53901
SSF53901   Thiolase-like
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[160-176] PS00606
PS00606   B_KETOACYL_SYNTHASE
SignalP
[1-19] 0.845 Signal
Eukaryota   
TMHMM No significant hit
Chro_00090 Chro_00090   Chro_00110 Chro_00110
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