Chro_00110 : CDS information

Location

Organism	Streptomyces griseus
Strain	ATCC 13273 (=NBRC 3746)
Entry name	Chromomycin
Contig	AJ578458
Start / Stop / Direction	12,201 / 11,221 / - [in whole cluster] 12,201 / 11,221 / - [in contig]
Location	complement(11221..12201) [in whole cluster] complement(11221..12201) [in contig]
Type	CDS
Length	981 bp (326 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative dTDP-glucose 4,6-dehydratase
Product (GenBank)	NDP-4,6-dehydratase
Gene
Gene (GenBank)	cmmE
EC number	4.2.1.46
Keyword	D-olivose D-oliose L-chromose
Note
Note (GenBank)
Reference	ACC Q70J87 PmId [15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004) comment aureolic acid type antitumor drug chromomycin A3の生合成gene clusterの同定論文。 CmmE: NDP-4,6-dehydratase 配列解析から機能推定。 CmmEはNDP-D-glucose 4,6-dehydratasesに似ている。 chromomycin deoxysugars生合成で共通する2nd stepを触媒すると提唱されている。
Related Reference	ACC Q56173 NITE Grana_00110 PmId [7476861] Identification of Streptomyces violaceoruber Tu22 genes involved in the biosynthesis of granaticin. (Mol Gen Genet. , 1995) [9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998) comment Blast 24th, id65%, 1e-112 Streptomyces violaceoruber_graE(gra-orf17) dTDP-glucose 4,6-dehydratase (EC 4.2.1.46) -- [PMID: 7476861](1995) abstract ORF2はstrEに似ている。 E.coliでORF2を発現させると、dTDP-glucose→dTDP-4-keto-6-deoxy-D-glucoseへの変換を触媒することができた(controlの5倍)。よって、ORF2はdTDP-glucose dehydrataseをコードする。 -- [PMID: 9831526](1998) gra cluster genesのまとめ。renameあり。 ORF2→ORF17 ORF3→ORF16

Sequence

>putative dTDP-glucose 4,6-dehydratase [NDP-4,6-dehydratase]
MRVLVTGGAGFIGSHYVRSLLSGAYAGYERAHVTVLDRLTYAGSTANLPMTHPRLTLVRG
DVCDRRPLLDLLPEHDAVVHFAAESHVDRSIHDASLFARTNVVGTQNLLDCCVRTGVRKV
VHVSTDEVYGSIDEGSWTEDHPLEPNSPYAASKAGADLMARAYGRTHGLDVSVTRCSNNY
GPHQHPEKLLPHFITRLLDGLQVPLYGDGRHVREWIHVDDHCRAVQLVLDKGAPGAAYNI
AGVARSNEDMTRDLLRLCGADWSRVRRVEDRKGHDRRYAVDDSLIRDTLGFTPRIPLEAG
LAGLVDWYRAHRAWWQPLTRTAQEAR

>putative dTDP-glucose 4,6-dehydratase [NDP-4,6-dehydratase]
ATGAGGGTCCTCGTCACCGGCGGCGCCGGTTTCATCGGGTCGCACTATGTGCGCAGCCTG
CTGTCCGGCGCCTACGCCGGGTACGAGCGAGCCCACGTCACCGTGCTGGACAGACTCACC
TACGCGGGCAGCACCGCCAACCTGCCGATGACCCACCCCCGGCTGACCCTGGTCCGGGGC
GACGTCTGCGACCGGCGACCGCTGCTCGATCTGCTGCCGGAACACGACGCGGTGGTGCAC
TTCGCGGCCGAGTCGCACGTGGACCGCTCCATCCACGACGCCTCCCTCTTCGCCCGTACG
AACGTGGTCGGCACCCAGAACCTGCTCGACTGCTGCGTGCGCACCGGTGTGCGGAAGGTG
GTGCACGTGTCGACCGACGAGGTCTACGGCTCCATCGACGAGGGCTCCTGGACGGAGGAC
CATCCGCTGGAACCCAACTCCCCCTACGCCGCCTCGAAGGCGGGGGCGGACCTCATGGCC
CGCGCCTACGGGCGCACCCACGGGCTCGACGTCTCGGTCACCCGCTGCTCCAACAACTAC
GGCCCCCACCAGCACCCGGAGAAGCTGCTCCCGCACTTCATCACCCGGCTCCTGGACGGG
CTCCAGGTGCCGCTGTACGGGGACGGCCGCCACGTACGCGAATGGATCCACGTGGACGAC
CACTGCCGCGCCGTCCAGCTCGTCCTGGACAAGGGAGCGCCGGGCGCGGCCTACAACATC
GCCGGCGTCGCGCGGTCCAACGAGGACATGACCCGCGACCTGCTGCGCCTGTGCGGCGCC
GACTGGAGCCGGGTGCGCAGGGTCGAGGACCGCAAGGGCCACGACCGGCGGTACGCGGTG
GACGACTCCCTGATCCGCGACACGCTCGGCTTCACACCCCGTATCCCGCTCGAAGCCGGA
CTGGCCGGCCTCGTCGACTGGTACCGCGCCCACCGGGCGTGGTGGCAACCGCTCACCCGC
ACCGCCCAGGAGGCCAGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR005888 dTDP-glucose 4,6-dehydratase (Family) TIGR01181 DTDP_gluc_dehyt IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site) PS00061 ADH_SHORT
SignalP	Eukaryota
TMHMM	No significant hit

Chro_00100 Chro_00120