Chro_00120 : CDS information

Location

Organism	Streptomyces griseus
Strain	ATCC 13273 (=NBRC 3746)
Entry name	Chromomycin
Contig	AJ578458
Start / Stop / Direction	13,265 / 12,198 / - [in whole cluster] 13,265 / 12,198 / - [in contig]
Location	complement(12198..13265) [in whole cluster] complement(12198..13265) [in contig]
Type	CDS
Length	1,068 bp (355 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative glucose-1-phosphate thymidylyltransferase putative dTDP-glucose synthase
Product (GenBank)	NDP-glucose synthase
Gene
Gene (GenBank)	cmmD
EC number	2.7.7.24
Keyword	D-olivose D-oliose L-chromose
Note
Note (GenBank)
Reference	ACC Q70J86 PmId [15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004) comment aureolic acid type antitumor drug chromomycin A3の生合成gene clusterの同定論文。 CmmD: NDP-glucose-synthase 配列解析から機能推定。 CmmDはNDP-D-glucose synthasesに似ている。 chromomycin deoxysugars生合成の共通する1st stepを触媒すると提唱されている。
Related Reference	ACC Q2HR55 NITE Sch475_00270 PmId [18051285] Cloning and expression of glucose-1-phosphate thymidylyltransferase gene (schS6) from Streptomyces sp. SCC-2136. (J Microbiol Biotechnol. , 2007) comment Blast 2nd, id58%, 1e-115 Streptomyces sp. SCC 2136_schS6 Putative dNDP-glucose synthetase schS6をE.coliで発現、精製、酵素活性測定。 glucose-1-phosphate thymidylyltransferase活性を示す。 dTTP + alpha-D-glucose-1-phosphate → dTDP-D-glucose + pyrophosphate

Sequence

>putative glucose-1-phosphate thymidylyltransferase [NDP-glucose synthase]
MKALVLAGGMGTRLRPLTHSMPKQLVPLANKPVLCHALEDIARAGVTDVGIIVGDWHKEI
EEAVGDGGRFGLRITYLPQEAPFGLAHCVLVAREFLADDDFLMYLGDNVFADGVHALADA
FRRDRPAAQVAVTAVDSPQEYGVAVTTSDGRVLGLQEKPREPRSDLAVTGAYFFTPEIHD
AIARIRPSTRGEWEITDAIQALIDRGSEVRAVRLSDWWKDTGRVEDILDCNQLLLDHVTT
SVRGSVDAASTVLGPVVVEPGARIIRSHLRGPLIVGAGCVVEDSYVGPYSAVGANCALTD
AGISHSIMLQDATVTDVNGIHRSVIGRSARVGAPVNGTGRHRLVVGDHTELEVVA

>putative glucose-1-phosphate thymidylyltransferase [NDP-glucose synthase]
GTGAAGGCTCTGGTTCTGGCCGGCGGAATGGGGACGCGGCTGCGCCCGCTCACCCACTCC
ATGCCGAAACAGCTGGTCCCCCTCGCGAACAAACCGGTGCTGTGCCACGCCCTGGAGGAC
ATCGCGCGGGCCGGTGTCACGGACGTCGGCATCATCGTGGGGGACTGGCACAAGGAGATC
GAGGAGGCGGTGGGGGACGGCGGGCGCTTCGGCCTGCGGATCACCTACCTGCCCCAGGAG
GCGCCCTTCGGCCTGGCCCACTGCGTACTGGTCGCGCGGGAGTTCCTGGCGGACGACGAC
TTCCTGATGTACCTCGGGGACAACGTCTTCGCCGACGGCGTCCACGCGCTCGCCGACGCC
TTCCGCCGGGACCGGCCGGCCGCCCAGGTCGCCGTGACCGCCGTGGACAGCCCCCAGGAG
TACGGCGTCGCCGTGACCACCTCGGACGGCCGGGTGCTCGGACTCCAGGAGAAGCCGCGC
GAGCCGAGGAGCGACCTCGCCGTGACCGGCGCCTACTTCTTCACCCCGGAGATCCACGAC
GCCATCGCCCGCATCCGGCCCAGCACACGCGGCGAGTGGGAGATCACCGACGCCATCCAG
GCCCTGATCGACCGGGGCAGCGAGGTGCGGGCGGTGCGGCTGTCCGACTGGTGGAAGGAC
ACCGGGCGCGTGGAGGACATCCTCGACTGCAATCAGCTCCTGCTCGACCACGTCACGACG
TCCGTCCGCGGTTCCGTTGACGCGGCGAGCACTGTGCTCGGTCCGGTCGTGGTCGAGCCC
GGCGCCCGCATCATCCGCTCGCACCTGCGCGGACCGCTGATCGTCGGCGCGGGCTGCGTG
GTCGAGGACAGCTATGTCGGCCCGTACTCCGCCGTCGGCGCGAACTGCGCCCTCACCGAC
GCGGGCATCTCCCACTCGATCATGCTGCAGGACGCGACCGTCACCGACGTCAACGGCATC
CACCGCTCGGTCATCGGCCGCTCAGCCCGGGTGGGCGCCCCCGTGAACGGCACGGGACGC
CACCGGCTCGTGGTGGGCGACCACACCGAACTCGAAGTCGTCGCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005835 Nucleotidyl transferase (Domain) PF00483 NTP_transferase IPR005908 Glucose-1-phosphate thymidylyltransferase, long form (Family) TIGR01208 RmlA_long
SignalP	Eukaryota
TMHMM	No significant hit

Chro_00110 Chro_00130