Chro_00120 Chro_00120   Chro_00140 Chro_00140

Chro_00130 : CDS information

close this sectionLocation

Organism
StrainATCC 13273 (=NBRC 3746)
Entry nameChromomycin
Contig
Start / Stop / Direction13,459 / 14,211 / + [in whole cluster]
13,459 / 14,211 / + [in contig]
Location13459..14211 [in whole cluster]
13459..14211 [in contig]
TypeCDS
Length753 bp (250 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductD-oliose C-4' O-methyltransferase
Product (GenBank)sugar O-methyltransferase
Gene
Gene (GenBank)cmmMIII
EC number2.1.1.-
Keyword
  • disaccharide 2nd D-oliose
Note
Note (GenBank)
Reference
ACC
PmId
[15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004)
[15255901] Tailoring modification of deoxysugars during biosynthesis of the antitumour drug chromomycin A by Streptomyces griseus ssp. griseus. (Mol Microbiol. , 2004)
comment
[PMID:15112992](2004)
aureolic acid type antitumor drug chromomycin A3の生合成gene clusterの同定論文。

CmmMIII : O-methyltransferase

配列解析から機能推定。
aglyconeに付着された糖をmethyl化するO-methyltransferaseに似ている。
よってCmmMIII は2糖鎖の末端D-olioseの4-O-methylationして4-O-methyl-D-olioseを生成する。

--
[PMID:15255901](2004)
cmmMIII不活化mutantはchromomycin非産生。
すべての糖を含むがacylatoionパターンが異なるmethyl化されていない派生体を蓄積。
このうち2つはcmmMIII を発現したS.albusで、chromomycin A2 and A3へと生物変換された。
Related Reference
ACC
Q9AJU0
NITE
Ello_00200
PmId
[11376004] Deoxysugar methylation during biosynthesis of the antitumor polyketide elloramycin by Streptomyces olivaceus. Characterization of three methyltransferase genes. (J Biol Chem. , 2001)
comment
Blast 6th, id62%, 7e-81
Streptomyces olivaceus_elmMIII
O-methyltransferase III (Rhamnose C4'-methyltransferase)

elloramycin aglycone合成cosmid cos16F4からelmMI, elmMII, and elmMIII を同定。
配列解析、DB比較、各geneの異種性発現、vitroでのmethylating activity測定。

これらの結果から、ElmMIII はaglyconeに付着した後の糖の4'-positionでOH基のメチル化をする。
各種糖基質に対する活性の違いから、C2', C3', C4'の順にメチル化されることもわかっている。

close this sectionSequence

selected fasta
>D-oliose C-4' O-methyltransferase [sugar O-methyltransferase]
MLSRGGELYLELMKRVLANVVYEDAPVGWYGDLDGYDTKARSQGLDLPTTAHTMVGLKRL
ANVQACVERVIRDKVPGDLIETGVWRGGVCILMRAVLEAYGVRDRRVWVADSFEGVPVVH
SASHPLDRDMALHRINGVLKISQEEVRENFSRYGLLDGQVDFLAGWFNETLPGAPVDSLA
VMRLDGDLYESTMDALTHLYPKLSVGGFVIIDDYNIEPCRQAVWDYRSRYGIEEPIEPVD
TCGVHWRRTG
selected fasta
>D-oliose C-4' O-methyltransferase [sugar O-methyltransferase]
ATGCTCTCAAGGGGTGGCGAGCTCTATCTGGAACTGATGAAACGGGTGCTCGCGAACGTG
GTCTACGAGGACGCCCCGGTGGGGTGGTACGGCGACCTCGACGGTTACGACACGAAGGCC
CGCTCGCAGGGACTCGACCTGCCCACGACGGCGCACACGATGGTCGGCCTGAAGCGTTTG
GCGAATGTGCAGGCGTGTGTCGAGCGGGTGATCCGCGACAAGGTCCCCGGCGACCTGATC
GAGACGGGGGTGTGGCGCGGCGGGGTCTGCATCCTGATGCGTGCGGTGCTGGAGGCGTAC
GGGGTGCGGGACCGCCGGGTGTGGGTGGCGGACTCGTTCGAGGGTGTGCCCGTCGTGCAC
TCCGCGAGTCATCCGCTGGACCGGGACATGGCGCTGCACCGCATCAACGGCGTTCTGAAG
ATCTCCCAGGAGGAGGTACGGGAGAACTTCTCCCGCTACGGACTGCTGGACGGCCAGGTC
GATTTCCTGGCGGGGTGGTTCAACGAGACTCTGCCCGGCGCACCCGTTGATTCCCTCGCG
GTGATGCGACTGGACGGGGATCTCTACGAGTCGACGATGGACGCGCTGACCCATCTGTAT
CCGAAGCTGTCGGTGGGCGGGTTCGTCATCATCGACGACTACAACATCGAGCCCTGCCGG
CAGGCGGTGTGGGACTACCGCAGCCGGTACGGCATCGAGGAGCCCATCGAGCCCGTCGAC
ACCTGCGGCGTGCACTGGCGCAGGACCGGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR008884 Macrocin-O-methyltransferase (Family)
[6-249] 3.70000000000004e-124 PF05711
PF05711   TylF
SignalP
[1-18] 0.308 Signal
Eukaryota   
TMHMM No significant hit
Chro_00120 Chro_00120   Chro_00140 Chro_00140
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