Chro_00140 : CDS information

Location

Organism	Streptomyces griseus
Strain	ATCC 13273 (=NBRC 3746)
Entry name	Chromomycin
Contig	AJ578458
Start / Stop / Direction	15,225 / 14,323 / - [in whole cluster] 15,225 / 14,323 / - [in contig]
Location	complement(14323..15225) [in whole cluster] complement(14323..15225) [in contig]
Type	CDS
Length	903 bp (300 aa)

Annotation

Category	4.1 transcriptional regulator
Product	putative transcriptional regulator
Product (GenBank)	transcriptional regulator
Gene
Gene (GenBank)	cmmRI
EC number
Keyword	SARP family
Note
Note (GenBank)
Reference	ACC Q70J84 PmId [15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004) comment aureolic acid type antitumor drug chromomycin A3の生合成gene clusterの同定論文。 CmmRI : Transcriptional activator 配列解析から機能推定。 SARP familyのtranscriptional activatorsに似ている。
Related Reference	ACC Q83X29 PmId [12791134] The large linear plasmid pSLA2-L of Streptomyces rochei has an unusually condensed gene organization for secondary metabolism. (Mol Microbiol. , 2003) [20378964] Regulation of lankamycin biosynthesis in Streptomyces rochei by two SARP genes, srrY and srrZ. (Biosci Biotechnol Biochem. , 2010) comment Blast 13th, id46%, 4e-54 Streptomyces rochei_srrY SARP family regulator SrrY --- [PMID: 12791134](2003) ORF75: SARP family regulatory protein Gene disruptionからLankacidin, Lankamycin産生に不可欠と確認。 conserved DNA-binding motifsのalignment fig.あり。 --- [PMID: 20378964](2010) 上記論文と同じ著者ら。 srrY geneはsrrZ mutantで転写されたが、srrZ geneはsrrY mutantで転写されなかった。 SrrY proteinは特異的にsrrZの promoter regionに結合することを確認。 srrZの相補はsrrY mutantにおいてlankamycin産生を回復した。 --->SARP gene srrYはpositive mannerでsecond SARP gene srrZの発現を直接的に制御する。
Related Reference	ACC P25047 NITE Adria_00210 PmId [1729206] Regulation of secondary metabolism in Streptomyces spp. and overproduction of daunorubicin in Streptomyces peucetius. (J Bacteriol. , 1992) [7868593] Functional characterization and transcriptional analysis of the dnrR1 locus, which controls daunorubicin biosynthesis in Streptomyces peucetius. (J Bacteriol. , 1995) [8971703] Purification and characterization of the DNA-binding protein DnrI, a transcriptional factor of daunorubicin biosynthesis in Streptomyces peucetius. (Mol Microbiol. , 1996) [16545946] Feedback regulation of doxorubicin biosynthesis in Streptomyces peucetius. (Res Microbiol. , 2006) [20529134] Modulation of dnrN expression by intracellular levels of DnrO and daunorubicin in Streptomyces peucetius. (FEMS Microbiol Lett. , 2010) [20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010) comment Blast 27th, id45%, 1e-50 Streptomyces peucetius_dnrI Regulatory protein dnrI -- [PMID: 1729206](1992) ATCC 29050 strainでdnrI を不活化したら、RHO産生とdaunorubicin (DNR)産生ができなくなり、DNRへの抵抗性が著しく減った。 --- 同じ著者らの続報。 [PMID: 7868593](1995) dnrI::aphII mutantでは、DNR生合成におけるearly- and late-acting enzymesの両方をコードする転写物が存在しない。よってdnrI は、直接または間接的にDNR生合成遺伝子のすべてを調節している。 dnrI::aphII and dnrN::aphII strainsでresistance genes=drrAB and drrC転写物は消失。よって、dnrI はDNR抵抗性の重大なregulatorであり、dnrNはdnrI を通して間接的にその影響を発現する、と示唆される。 -- [PMID: 8971703](1996) abstract DNA-binding assays. ・dnrG-dpsABCD と dpsEF の間・dnrC gene と dnrDKPSQ の間の遺伝子間領域を含んだDNA断片へ特異的に結合する。 -- [PMID: 16545946](2006) abstract dnrI promoter活性を増やすためにはDnrO and DnrN の両方が必要であった。 -- [PMID: 20529134]や[PMID: 20158521]で、dnrO, dnrN, dnrI, DNRが関与する細胞内DNR濃度維持システムが提唱されている。 dnrI promoterにはDnrNが結合し活性化される。

Sequence

>putative transcriptional regulator [transcriptional regulator]
MTYGYCTERIEFQLLGSLGLRTSDHPNLADELTAPKPRKIIALLVMQANHIVPTTAMARE
LWGDNPPASVQTTLQTYVLQARRLLGRALCLSPADVARRILVTKFGGYQLNVSHGSVDVN
EFARLAAEGQHAFLEGEDERAVRLLTEALDLWSGPPLVDIQAGPLLSAEIRRLEENRLTV
QMQMVDAKLRLALHDQLPGELAGLAAQYPRNENLHAQYMLALYRCGRCPDALDVFRRLRE
RLVDELGLEPSLKIQALHRAMLAADPALDHGAVPTEGNLLLDRFAPTAPGREPWATRRAG

>putative transcriptional regulator [transcriptional regulator]
GTGACCTACGGCTACTGCACGGAACGCATCGAGTTTCAGTTACTTGGTTCACTCGGCTTG
CGGACGTCCGACCACCCCAACCTGGCGGACGAACTCACTGCCCCCAAGCCGCGGAAGATC
ATCGCCTTGCTGGTCATGCAGGCGAACCACATCGTGCCCACGACGGCGATGGCTCGGGAA
CTGTGGGGCGACAACCCGCCCGCCAGCGTTCAGACCACGCTGCAGACCTACGTCCTCCAG
GCCCGCAGACTCCTCGGTCGCGCCCTGTGCCTCTCGCCCGCGGACGTCGCCCGGCGGATC
CTCGTCACCAAGTTCGGCGGTTACCAGCTGAACGTCAGTCACGGCTCCGTCGACGTGAAC
GAGTTCGCCCGGCTGGCCGCCGAGGGCCAGCACGCGTTCCTCGAGGGTGAGGACGAGAGA
GCCGTCCGGTTGCTGACCGAGGCCCTCGACCTGTGGAGCGGACCGCCGCTCGTAGACATC
CAGGCGGGACCGCTGCTGTCCGCGGAGATACGGCGCCTGGAGGAGAACCGGCTCACGGTG
CAGATGCAGATGGTCGACGCCAAGCTCCGTCTCGCCCTGCACGACCAGCTTCCGGGCGAA
CTGGCGGGGCTGGCCGCCCAGTACCCGAGGAACGAGAACCTGCACGCCCAGTACATGCTC
GCGCTGTACCGCTGCGGCCGCTGTCCGGACGCTCTGGACGTGTTCCGGCGGTTGCGCGAG
CGGCTCGTCGACGAGCTGGGCCTGGAGCCCTCGCTCAAGATCCAGGCCCTGCACCGGGCG
ATGCTCGCAGCCGATCCCGCCCTCGACCACGGGGCGGTCCCCACCGAAGGCAACCTGCTG
CTCGACCGGTTCGCCCCCACCGCCCCCGGCCGTGAGCCGTGGGCCACCCGCCGGGCCGGC
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001867 Signal transduction response regulator, C-terminal (Domain) SM00862 Trans_reg_C PF00486 Trans_reg_C IPR005158 Bacterial transcriptional activator domain (Domain) PF03704 BTAD SM01043 BTAD IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR016032 Signal transduction response regulator, C-terminal effector (Domain) SSF46894 Bipartite_resp_reg_C-effector
SignalP	Bacteria, Gram-positive Bacteria, Gram-negative
TMHMM	No significant hit

Chro_00130 Chro_00150