Chro_00140 Chro_00140   Chro_00160 Chro_00160

Chro_00150 : CDS information

close this sectionLocation

Organism
StrainATCC 13273 (=NBRC 3746)
Entry nameChromomycin
Contig
Start / Stop / Direction15,688 / 16,716 / + [in whole cluster]
15,688 / 16,716 / + [in contig]
Location15688..16716 [in whole cluster]
15688..16716 [in contig]
TypeCDS
Length1,029 bp (342 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.2 modification methylation
Productputative C-methyltransferase
Product (GenBank)C-methyltransferase
Gene
Gene (GenBank)cmmMII
EC number2.1.1.-
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004)
comment
aureolic acid type antitumor drug chromomycin A3の生合成gene clusterの同定論文。

CmmMII : C-methyltransferase

配列解析から機能推定。
4環中間体の9-C-methylationを担うMtmMII に似ている。
mithramycinと同じaglyconeを持つchromomycin生合成でも同じ機能を担うと提唱されている。
おそらくtrisaccharideが形成された後に起こる。
Related Reference
ACC
Q194P8
NITE
Mith_00270
PmId
[10652287] Characterization of two polyketide methyltransferases involved in the biosynthesis of the antitumor drug mithramycin by Streptomyces argillaceus. (J Biol Chem. , 2000)
[14660589] MtmMII-mediated C-methylation during biosynthesis of the antitumor drug mithramycin is essential for biological activity and DNA-drug interaction. (J Biol Chem. , 2004)
comment
Blast 2nd, id52%, 3e-91
Streptomyces argillaceus_mtmMII
C-methyltransferase

--
[PMID:10652287](2000)
不活化mutant作成、異種性発現・精製・活性測定により、MtmMI は4-demethylpremithramycinoneの4-O-methylationを、MtmMII は9-demethylpremithramycin A3の9-C-methylationを担うことが実証されている。

--
[PMID:14660589](2004)
MtmMII が担う芳香環methyl化と生物学的活性との関連を調査。

close this sectionSequence

selected fasta
>putative C-methyltransferase [C-methyltransferase]
MTKDHIPTVTRQGLLTMMSAVRRTGILRAGVELRVFDAVAGAPAGPDEVAAFCGTDPRGM
RILLNALAATGLLVKDDDRFSLAPGADSTLVTTHPDYYGDAVRLAASDYEWDAIKRLGDA
VRSGGSVMEVNAETPAYTFWEDFAALGTLNTPPIAELVARHVLPGTKERDEVKVLDIACG
HGLYGYTLAAHDPRARIWSLDWPNVLAVTENNAERLGVRDRAHFIPGDMFEVPLGGPYDV
VVVANVLHHFSTERAKRLLARTADAVRPGGQLVAIAISTGDGPPHLDPVPHLFSALMLAW
TSQGQVHPLSDYEEMVTAAGFAPPKVHGLPDNPLRVLIADRV
selected fasta
>putative C-methyltransferase [C-methyltransferase]
ATGACCAAGGACCACATACCCACCGTCACCCGCCAGGGCCTGCTGACCATGATGTCCGCG
GTACGCAGGACGGGCATCCTCCGTGCCGGGGTCGAACTACGGGTGTTCGACGCCGTGGCC
GGAGCGCCGGCCGGCCCGGACGAGGTCGCCGCCTTCTGCGGAACCGACCCGCGCGGCATG
CGCATCCTGCTCAACGCCCTCGCCGCGACAGGCCTGCTGGTGAAGGACGACGACCGGTTC
TCGCTCGCGCCGGGCGCCGACAGCACCCTGGTCACCACCCACCCTGACTACTACGGCGAC
GCCGTGCGGCTTGCCGCCAGCGACTACGAGTGGGACGCGATCAAGCGCCTCGGCGACGCG
GTGCGCTCGGGCGGTTCCGTGATGGAGGTGAACGCCGAGACACCCGCGTACACGTTCTGG
GAGGACTTCGCCGCGCTGGGCACCCTGAACACCCCGCCGATCGCGGAGCTGGTCGCTCGC
CACGTGCTGCCCGGGACGAAGGAGCGCGACGAGGTGAAGGTGCTCGACATCGCCTGCGGG
CACGGGCTCTACGGGTACACCCTGGCCGCCCACGATCCCCGCGCCCGGATCTGGTCCCTG
GACTGGCCCAACGTCCTGGCCGTCACCGAGAACAACGCGGAGAGGCTCGGGGTGCGCGAC
CGCGCGCACTTCATCCCCGGTGACATGTTCGAGGTACCGCTCGGAGGGCCGTACGACGTG
GTGGTCGTCGCCAACGTCCTGCACCACTTCTCCACCGAGCGGGCGAAGCGACTGCTGGCC
AGGACGGCCGACGCGGTCCGGCCCGGCGGACAGCTCGTCGCCATCGCGATCAGCACCGGC
GACGGGCCGCCGCACCTTGACCCCGTACCCCACCTGTTCTCGGCCCTGATGCTGGCCTGG
ACCTCCCAGGGGCAGGTGCACCCCCTCTCCGACTACGAGGAGATGGTCACCGCCGCCGGT
TTCGCGCCACCGAAGGTCCACGGCCTCCCGGACAACCCGCTGCGCGTGCTCATCGCCGAC
CGTGTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001077 O-methyltransferase, family 2 (Domain)
[79-315] 4.6e-14 PF00891
PF00891   Methyltransf_2
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[8-87] 1.99999999999999e-24 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016461 O-methyltransferase, caffeic acid-type (Family)
[13-341] 0.000170000015610474 PIRSF005739
PIRSF005739   O-mtase
SignalP No significant hit
TMHMM No significant hit
Chro_00140 Chro_00140   Chro_00160 Chro_00160
Page top