Chro_00270 : CDS information

Location

Organism	Streptomyces griseus
Strain	ATCC 13273 (=NBRC 3746)
Entry name	Chromomycin
Contig	AJ578458
Start / Stop / Direction	31,035 / 29,965 / - [in whole cluster] 31,035 / 29,965 / - [in contig]
Location	complement(29965..31035) [in whole cluster] complement(29965..31035) [in contig]
Type	CDS
Length	1,071 bp (356 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose C-4 ketoreductase
Product (GenBank)	NDP-4-ketoreductase
Gene
Gene (GenBank)	cmmUIII
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q70J71 PmId [15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004) comment aureolic acid type antitumor drug chromomycin A3の生合成gene clusterの同定論文。 CmmUIII : NDP-4-ketoreductase 配列解析から機能推定。 L-oleandrose生合成に関与するAveBIVなど、L-sugars生合成での4-ketoreductasesに似ている。よってCmmUIII は4-O-acetyl-L-chromoseの生合成に関連した4-ketoreductaseであると提唱されている。
Related Reference	ACC Q2PC38 NITE Rubra_00520 PmId [16614891] Functional characterization of ketoreductase (rubN6) and aminotransferase (rubN4) genes in the gene cluster of Streptomyces achromogenes var. rubradiris. (Biotechnol Lett. , 2006) comment Blast 6th, id53%, 7e-58 Streptomyces achromogenes subsp. rubradiris_rubN6 Putative 4-Ketoreductase rubN6 and rubN4をHis-tagged fusion proteinsとしてE.coliで異種性に発現。それぞれのdTDP-glucose 4-ketoreductase and dTDP-glucose 3-aminotransferaseの機能性を、in vitro enzyme assayによって検証。D-rubranitrose生合成に関与。
Related Reference	ACC Q9F834 NITE Megalo_00080 PmId [20418422] TDP-L-megosamine biosynthesis pathway elucidation and megalomicin a production in Escherichia coli. (Appl Environ Microbiol. , 2010) comment Blast 16th, id48%, 1e-50 Micromonospora megalomicea subsp. nigra_megDV TDP-4-keto-6-deoxyhexose 4-ketoreductase 異なる遺伝子セットを含んだオペロンによってE.coliで産生されたdTDP-sugar中間体のLC/MS/MS解析。 MegDVがL-megosamine生合成に特異的に関与する4-ketoreductaseであることを確認。

Sequence

>putative NDP-hexose C-4 ketoreductase [NDP-4-ketoreductase]
MAGEKTEPLVVVLGAAGYLGSAVVAELARRPVRLRRVARPGRLREIPAAVWGDASSQASP
WAADLSRPGAVARAVAGADVIVHLVAHIGEASAWRAAEHDPAAARVNVEPAHDLVAALRA
RRGGGPPPVVLFAGSASQVGRAGVIDGSEPDEPVTVYARQKLAAEQALLAASAEGVLRGV
SLRLPTVYGAGPGPLGNGVVTAMARRAFAGEPLPVWSDGSVERDLLHVTDAARAFAVCAA
PDRAGALAGRHWVVGTGRPVRVADLFTAVARSVAAHTGAPPVPVVSVEPPATATAADVRG
TVVDASAFRTATAGAPGSRCRRRWTSWWRPSPGRGQSDDGPQDRGGVPQTMWSSGT

>putative NDP-hexose C-4 ketoreductase [NDP-4-ketoreductase]
GTGGCAGGGGAGAAGACGGAGCCGCTGGTCGTCGTCCTGGGCGCTGCCGGCTATCTGGGA
TCGGCCGTCGTGGCGGAGCTCGCCCGCCGGCCGGTCCGGCTGCGCCGGGTGGCCCGGCCC
GGCCGGCTGCGCGAGATCCCAGCGGCGGTCTGGGGGGACGCCTCGTCGCAGGCGTCGCCG
TGGGCGGCCGACCTGTCCCGGCCCGGGGCCGTCGCCCGGGCCGTGGCGGGCGCCGACGTG
ATCGTTCATCTCGTCGCGCACATCGGCGAGGCGTCGGCATGGCGGGCCGCCGAACACGAC
CCGGCCGCCGCGCGGGTGAACGTGGAGCCGGCACACGACCTGGTGGCCGCGCTCCGGGCG
AGGCGGGGCGGCGGGCCGCCACCGGTGGTGCTCTTCGCCGGTTCGGCCTCACAGGTGGGG
CGGGCGGGGGTGATCGACGGCAGCGAACCCGACGAGCCCGTAACGGTGTACGCACGCCAG
AAGCTCGCCGCTGAGCAGGCTCTCCTGGCCGCTTCGGCCGAGGGGGTGCTGCGCGGGGTG
TCGCTGCGGCTGCCCACGGTGTACGGGGCGGGGCCCGGGCCGCTCGGCAACGGGGTGGTC
ACCGCGATGGCGCGGCGCGCGTTCGCGGGCGAGCCGCTCCCGGTGTGGAGCGACGGGTCC
GTGGAACGGGACCTGCTGCATGTCACGGACGCGGCCCGCGCGTTCGCGGTGTGCGCGGCC
CCGGACCGGGCCGGCGCGCTGGCCGGACGTCACTGGGTGGTGGGCACCGGCCGGCCGGTG
CGGGTGGCCGATCTGTTCACCGCCGTCGCCCGGAGCGTCGCCGCGCACACCGGCGCGCCG
CCGGTGCCGGTGGTGTCCGTGGAGCCGCCGGCCACGGCGACGGCCGCCGACGTCCGGGGC
ACGGTCGTCGACGCCTCGGCCTTCCGGACCGCCACGGCTGGCGCCCCCGGCAGTCGATGC
CGGAGGCGCTGGACGAGCTGGTGGCGGCCCTCGCCCGGCAGAGGGCAGTCTGACGACGGG
CCCCAGGACCGTGGTGGGGTCCCTCAGACGATGTGGAGTTCGGGCACGTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	Eukaryota Bacteria, Gram-positive
TMHMM	No significant hit

Chro_00260 Chro_00280