Chro_00250 Chro_00250   Chro_00270 Chro_00270

Chro_00260 : CDS information

close this sectionLocation

Organism
StrainATCC 13273 (=NBRC 3746)
Entry nameChromomycin
Contig
Start / Stop / Direction28,766 / 29,992 / + [in whole cluster]
28,766 / 29,992 / + [in contig]
Location28766..29992 [in whole cluster]
28766..29992 [in contig]
TypeCDS
Length1,227 bp (408 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose C-3 C-methyltransferase
Product (GenBank)NDP-C-methyltransferase
Gene
Gene (GenBank)cmmC
EC number2.1.1.-
Keyword
  • L-chromose
Note
Note (GenBank)
Reference
ACC
PmId
[15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004)
comment
aureolic acid type antitumor drug chromomycin A3の生合成gene clusterの同定論文。

CmmC: NDP-C-methyltransferase

配列解析から機能推定。
C-3でaxialなC-methyl基をもたらすC-methyltransferaseに似ていることから、4-O-acetyl-L-chromoseの生合成に必要なC-methyltransferaseであると提唱されている。
Related Reference
ACC
P96561
NITE
Chlor_00150
PmId
[11035791] Deoxysugars in glycopeptide antibiotics: enzymatic synthesis of TDP-L-epivancosamine in chloroeremomycin biosynthesis. (Proc Natl Acad Sci U S A. , 2000)
comment
BLAST id63%
Amycolatopsis orientalis
PCZA361.22
ORF14(EvaC)に相当するentry.

--
[PMID: 11035791](2000)
EvaC(ORF14)を含むdTDP-L-epivancosamine合成に関連する5つのタンパクをE.coliで発現、精製。
これらと基質を組み合わせて反応させ、産物のmass spectrometry and NMRによって各酵素活性、dTDP-L-epivancosamine生合成経路を同定している。
EvaC(ORF14)は、dTDP-3-amino-2,3,6-trideoxy-D-threo-hexopyranos-4-uloseのC-3にequatorialなmethyl基を形成する。
C-3 methyl基をaxialにするのは、この後の反応が関連している。

close this sectionSequence

selected fasta
>putative NDP-hexose C-3 C-methyltransferase [NDP-C-methyltransferase]
MSDTLDCRICGGTLHEFFDFGRQPLADAFVDPNHPAQEYFHRLAVAQCGSCTMVQLLEDV
PREHMFRTDYPYLSSGSSAMQDHFRATAGHFLDHELRDLDSFVVEFGSNDGIMLDTVARA
GRRHLGVEPCAGVAEAAAAKGIRVRNAFFEESTAAEIVATEGRANVIYAANTFSHISYVD
SVFRGVDILLDEDGIFVFEDPYMGDIVERTSFDQIYDEHFYFFTVRSVQNMAAFYGFELV
DVEHLPVHGGEIRYTLARSGSRTPSPEVARFLDREKILGLAERPTLESFATNIDLLREGL
RSLLVRCRDEGRSVVGYGATAKSATLTNYCGIGPELVSFIADNTPAKQGTLSPGSHIPVR
SPEAFADPYPDYALLFAWNHAEEIMRKEAEFKRRGGRWILYVPELHIV
selected fasta
>putative NDP-hexose C-3 C-methyltransferase [NDP-C-methyltransferase]
ATGTCTGACACGCTCGACTGCCGGATCTGCGGCGGTACGCTGCACGAGTTCTTCGACTTC
GGACGCCAGCCACTGGCTGACGCGTTCGTCGACCCGAACCACCCCGCCCAGGAGTACTTC
CACCGGCTAGCGGTTGCCCAGTGCGGCTCCTGCACGATGGTCCAGCTCCTGGAGGACGTA
CCGCGCGAGCACATGTTCCGCACCGACTACCCCTACCTCTCCTCGGGGTCCTCGGCCATG
CAGGACCACTTCCGCGCCACCGCCGGGCACTTCCTCGACCACGAACTGCGCGACCTGGAC
TCCTTCGTCGTCGAGTTCGGCAGCAACGACGGCATCATGCTCGACACCGTGGCCCGGGCC
GGCCGCCGCCACCTCGGCGTCGAGCCCTGCGCGGGCGTCGCCGAGGCCGCCGCCGCCAAG
GGCATCCGGGTCCGTAACGCCTTCTTCGAGGAATCCACGGCGGCCGAGATCGTCGCCACC
GAGGGCCGCGCGAACGTCATCTACGCCGCCAACACCTTCTCGCACATCTCCTACGTCGAC
TCCGTCTTCCGCGGGGTGGACATCCTGCTCGACGAGGACGGCATCTTCGTCTTTGAGGAC
CCCTACATGGGGGACATCGTCGAGCGGACGTCGTTCGACCAGATCTACGACGAGCACTTC
TACTTCTTCACCGTGCGGTCGGTGCAGAACATGGCGGCCTTCTACGGATTCGAACTCGTC
GACGTGGAGCACCTGCCCGTGCACGGCGGCGAGATCCGCTACACGCTCGCCCGGTCGGGC
TCACGGACGCCCTCCCCCGAGGTCGCCCGCTTCCTGGACCGGGAGAAGATCCTCGGACTC
GCCGAACGGCCCACGCTGGAGAGCTTCGCCACCAACATCGACCTGCTGCGCGAGGGGCTC
CGCTCGCTGCTGGTGCGCTGCCGTGACGAGGGCAGGAGCGTCGTCGGCTACGGGGCGACC
GCCAAGAGCGCCACGCTCACCAACTACTGCGGCATCGGCCCCGAACTGGTGTCCTTCATC
GCGGACAACACCCCGGCCAAGCAGGGCACACTCAGTCCCGGATCGCACATCCCGGTGCGC
TCGCCCGAGGCGTTCGCCGACCCCTACCCCGACTACGCGCTGCTGTTCGCCTGGAACCAC
GCCGAGGAGATCATGCGCAAGGAGGCCGAGTTCAAGCGCCGCGGCGGCCGGTGGATCCTC
TACGTGCCCGAACTCCACATCGTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR013630 Methyltransferase putative zinc binding domain (Domain)
[7-67] 4.29999999999998e-21 PF08421
PF08421   Methyltransf_13
IPR013691 C-methyltransferase (Domain)
[245-403] 4.80000000000003e-56 PF08484
PF08484   Methyltransf_14
SignalP No significant hit
TMHMM No significant hit
Chro_00250 Chro_00250   Chro_00270 Chro_00270
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