Chro_00360 : CDS information

Location

Organism	Streptomyces griseus
Strain	ATCC 13273 (=NBRC 3746)
Entry name	Chromomycin
Contig	AJ578458
Start / Stop / Direction	40,362 / 41,948 / + [in whole cluster] 40,362 / 41,948 / + [in contig]
Location	40362..41948 [in whole cluster] 40362..41948 [in contig]
Type	CDS
Length	1,587 bp (528 aa)

Annotation

Category	5.1 general function
Product	putative ligase
Product (GenBank)	acyl CoA ligase
Gene
Gene (GenBank)	cmmLII
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q70J62 PmId [15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004) comment aureolic acid type antitumor drug chromomycin A3の生合成gene clusterの同定論文。 CmmLII : Acyl-CoA ligase 配列解析から機能推定。 acyl CoA ligasesに似たタンパクをコードする。 AMP binding domain(Pfam00501)を含む。
Related Reference	ACC P41636 PmId [9008388] Molecular cloning of 4-coumarate:coenzyme A ligase in loblolly pine and the roles of this enzyme in the biosynthesis of lignin in compression wood. (Plant Physiol. , 1997) comment 463rd, id29%, 3e-51 Pinus taeda(テーダマツ)_4CL 4-coumarate--CoA ligase(EC 6.2.1.12) 圧縮ストレスにより木部での発現変化する 4CL activity with 4-coumaric acid: 上昇 with ferulic acid: 変化なし with caffeic acid: 低下

Sequence

>putative ligase [acyl CoA ligase]
MPFDLARVPEVPLDGLLRRAAAAAPDRVAVRTPTGTTTYAELDGRADDCARSLSRAAGGS
GTAVGLVASLDPSFAVAYYGAVRADHVVVLINPHLRGDALAHVLSVAGTTVVVAPPEFAE
RVAQVRGKLPHLRDVLSPDVLVQGRAGPGGRGRDAPGSAPHLDSVACVQFTSGTTGEPKA
VQLTHRNLVVNAAQIASVHGLDGDAVTLNNLPLYHPMHLNAAVYAGATQVLCPSPDPAEA
IEAANRHRATHYYSLPVRLAHLAADPRLPGLRLETVRAVFSGGSALLPAQARTLGTHFGI
PVVQGYGLAETSPLTHGEPPARPRPGSVGPEVPGTECRVVDMDRRTPLPAGRDGEIQVRG
PQLMRGYLGAAGPAVDADGWFSTGDIGHRDQDGYLYLVDRIKDVFKYENWLVSPTEIEQV
LITHPAVRDCAVVDHPEPFSGAVAHAFVVLDEDAALPGPAELAAHVNDQVPYYQQIKYLD
VVERVPRSPNGKILRRELRARAAHTDRADRLHPGAAGRPHSDLIGENP

>putative ligase [acyl CoA ligase]
GTGCCGTTCGACCTCGCGCGGGTGCCGGAGGTGCCCCTGGACGGTCTCCTGCGCCGGGCC
GCCGCGGCGGCGCCCGACCGGGTCGCGGTGCGCACCCCGACGGGGACGACGACGTACGCG
GAACTGGACGGCCGAGCCGACGACTGCGCCAGGTCCTTGAGCCGGGCGGCCGGCGGAAGC
GGCACCGCCGTCGGGCTGGTGGCCTCCCTCGACCCGTCGTTCGCGGTCGCCTACTACGGC
GCCGTGCGCGCGGACCACGTCGTCGTGCTGATCAACCCGCACCTGCGGGGTGACGCCCTC
GCCCACGTCCTCTCCGTCGCAGGGACGACGGTGGTGGTGGCCCCGCCCGAGTTCGCCGAG
CGGGTCGCCCAGGTGCGGGGGAAGCTCCCGCACCTGCGTGACGTCCTCTCCCCGGACGTG
CTCGTCCAGGGCCGCGCGGGGCCCGGCGGAAGGGGCCGCGATGCCCCGGGGAGCGCCCCG
CACCTGGACTCGGTGGCGTGCGTCCAGTTCACCAGCGGGACCACGGGGGAACCGAAGGCC
GTTCAGCTGACACACCGCAACCTGGTGGTCAACGCCGCACAGATCGCCTCCGTCCACGGC
CTGGACGGCGACGCGGTGACCCTCAACAACCTGCCGCTCTACCACCCGATGCACCTCAAC
GCCGCGGTGTACGCGGGAGCGACCCAGGTGCTGTGCCCGTCACCCGATCCGGCGGAGGCG
ATCGAGGCCGCGAACCGCCACCGCGCGACGCACTATTACAGCCTTCCCGTACGGCTGGCG
CACCTGGCGGCCGACCCCCGGCTGCCGGGCCTCCGGCTGGAGACGGTCCGCGCGGTCTTC
TCCGGCGGTTCGGCGCTGCTGCCCGCCCAGGCCCGTACCCTGGGGACGCACTTCGGCATC
CCGGTCGTGCAGGGGTACGGACTGGCCGAGACCTCCCCGCTCACACACGGCGAGCCGCCG
GCCCGGCCCCGGCCCGGCTCCGTGGGCCCTGAGGTGCCCGGCACCGAATGCCGGGTGGTG
GACATGGACCGGCGCACCCCGCTCCCGGCCGGGCGGGACGGCGAGATCCAGGTCCGTGGA
CCGCAGCTCATGCGTGGCTACCTCGGCGCCGCCGGCCCCGCGGTCGACGCCGACGGCTGG
TTCTCCACCGGTGACATCGGCCACCGGGACCAGGACGGCTACCTGTACCTGGTGGACCGG
ATCAAGGACGTCTTCAAGTACGAGAACTGGCTGGTGTCACCCACGGAGATCGAGCAGGTC
CTCATCACGCACCCGGCCGTACGGGACTGCGCGGTGGTCGACCACCCGGAACCTTTCAGC
GGCGCGGTGGCCCACGCCTTCGTCGTCCTCGACGAGGATGCCGCACTGCCCGGTCCGGCG
GAGCTCGCCGCCCATGTGAACGATCAGGTCCCCTACTACCAGCAGATCAAGTACCTCGAC
GTGGTGGAACGCGTCCCCCGTTCCCCGAACGGCAAGATCCTCCGCCGCGAACTGCGCGCA
CGTGCCGCGCACACCGACCGGGCCGACCGCCTGCACCCCGGCGCGGCCGGCCGTCCTCAC
TCCGACCTCATAGGAGAGAACCCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000873 AMP-dependent synthetase/ligase (Domain) PF00501 AMP-binding IPR020845 AMP-binding, conserved site (Conserved_site) PS00455 AMP_BINDING IPR025110 Domain of unknown function DUF4009 (Domain) PF13193 DUF4009
SignalP	No significant hit
TMHMM	No significant hit

Chro_00350 last last