Cosmo_00100 Cosmo_00100   Cosmo_00120 Cosmo_00120

Cosmo_00110 : CDS information

close this sectionLocation

Organism
StrainDAUFPE 5622
Entry nameCosmomycin
Contig
Start / Stop / Direction9,440 / 10,690 / + [in whole cluster]
9,440 / 10,690 / + [in contig]
Location9440..10690 [in whole cluster]
9440..10690 [in contig]
TypeCDS
Length1,251 bp (416 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative glycosyltransferase helper protein
Product (GenBank)CosT
Gene
Gene (GenBank)cosT
EC number
Keyword
  • L-rhodosamine
Note
Note (GenBank)
  • putative L-rhodosamine transfer; similar to DnrQ/AknT/DesVIII cytochrome P450-like proteins
Reference
ACC
PmId
[16810496] Insights in the glycosylation steps during biosynthesis of the antitumor anthracycline cosmomycin: characterization of two glycosyltransferase genes. (Appl Microbiol Biotechnol. , 2006)
comment
cosmomycinsを含むanthracycline複合体retamycinの産生株Streptomyces olindensisの染色体14kb領域から、cosmomycin生合成に関連した13genesを同定。

cosT: L-rhodosamine transfer

DesVIII, AknTといったglycosyltransferaseと協力するglycosylation必須タンパクに似ている。
よって、CosTは対応するglycosyltransferaseによるcosmomycin aglyconeへのL-rhodosamine移動に必須である、と提唱。
Related Reference
ACC
Q9L4U5
NITE
Acla_00220
PmId
[15911373] AknT is an activating protein for the glycosyltransferase AknS in L-aminodeoxysugar transfer to the aglycone of aclacinomycin A. (Chem Biol. , 2005)
[17685523] Characterization of rhodosaminyl transfer by the AknS/AknT glycosylation complex and its use in reconstituting the biosynthetic pathway of aclacinomycin A. (J Am Chem Soc. , 2007)
comment
Blast 3rd, id45%, 3e-76
Streptomyces galilaeus_aknT
AknT
[DoBISCUIT]glycosyltransferase helper protein

---
[PMID: 15911373](2005)
AknKの実験に基づき代替基質として2-deoxyfucoseを用いたAknS/Tの実験。
AknS単独でも活性はあるが、AknTがないと活性は低い。

---
[PMID: 17685523](2007)
dTDP-L-rhodosamineがAknS/AknTの自然な基質であることを確認。
これまでに代替基質として使用されていたdTDP-2-deoxyfucoseより100倍、dTDP-daunosamineより1000倍速いKcat値を示す。

partner protein AknTは、L-rhodosamine移動のためのAknSの代謝回転速度を200倍に加速する。
AknTの存在はKm値に影響せず、kcatに影響する。AknS:AknTの最適比率は1:3。

aglycone, L-rhodosamine, 2-deoxyfucose, AknS/T, AknKでの産物は2糖型aclacinomycinが主要産物でだったことから、3糖目は2-deoxyfucoseでなく、これまでの提唱通りL- rhodinoseでありそう。

close this sectionSequence

selected fasta
>putative glycosyltransferase helper protein [CosT]
MLTDQTNSELGRHLLTIRGVQFIFGAQGDPFAMILRAGDDDPLDHGARAREQGLHESSIG
AWVTAHRELGARILGDERFSPRHAEEGPQAHPGVDVWDNPLLCHVVPLDDAQLNRDPADY
RALARAAAPLLSAEALAARLPEIEAAYRQALAATGERFDLYRDVAVPAALRVVADVLGLP
EETRQRLPELHAGLATALDAGHCPQQLAAARALFTAVTDLSTELTRLVGSGRATGLAALD
GVSPEDAVAVGTLAVVVGVEVTATAVCNAVRVLLDHREEWERIGREPERAGAAVREALRL
APPLRFESRIAREQAEFDGKTVEEGRRVVVLVDAANRDPEAFADPDAFVPGRADGDRSLT
LLGGSAAPVTEALVRLQTEAAVRVLAELRPGLRTGAEVLHRMRSAVLRGVLRFPVA
selected fasta
>putative glycosyltransferase helper protein [CosT]
ATGCTGACCGATCAGACCAACAGCGAGTTGGGCCGGCATCTGCTGACCATCCGAGGCGTC
CAGTTCATCTTCGGCGCCCAGGGCGACCCCTTCGCCATGATCCTGCGCGCCGGGGACGAC
GACCCCCTCGACCACGGGGCGCGCGCCCGCGAACAGGGCCTGCACGAGAGCAGCATCGGC
GCGTGGGTCACCGCGCACCGCGAGCTCGGCGCCCGGATCCTCGGCGACGAGCGGTTCTCC
CCGCGCCACGCCGAGGAGGGGCCGCAGGCCCACCCGGGTGTCGACGTCTGGGACAACCCC
CTGCTGTGCCACGTCGTCCCCCTCGACGACGCCCAGCTCAACCGCGATCCGGCCGACTAC
CGCGCGCTGGCCCGGGCCGCGGCCCCGCTGCTGTCGGCCGAGGCGCTCGCCGCGCGGCTG
CCGGAGATCGAGGCGGCCTACCGCCAGGCGCTCGCCGCGACCGGCGAACGGTTCGACCTG
TACCGGGACGTCGCCGTCCCGGCCGCGCTGCGCGTGGTGGCCGACGTGCTCGGCCTGCCC
GAGGAGACGCGGCAGCGCCTGCCCGAACTGCACGCGGGTCTCGCCACCGCCCTGGACGCC
GGCCACTGCCCGCAGCAACTGGCGGCCGCCCGGGCGCTGTTCACGGCCGTGACGGACCTC
TCCACCGAGCTGACCCGCCTGGTCGGCAGCGGCCGCGCCACCGGCCTCGCCGCGCTCGAC
GGCGTGTCGCCCGAGGACGCGGTCGCCGTCGGGACGCTCGCCGTCGTGGTGGGCGTCGAG
GTCACCGCGACCGCCGTGTGCAACGCGGTGCGGGTCCTCCTGGACCACCGCGAGGAGTGG
GAGCGGATCGGGCGGGAGCCCGAACGGGCCGGCGCCGCGGTCCGCGAGGCCCTGCGGCTG
GCCCCGCCGCTGCGGTTCGAGAGCCGTATCGCGCGCGAGCAGGCCGAGTTCGACGGCAAG
ACCGTCGAGGAGGGCCGGCGCGTCGTGGTGCTCGTCGACGCGGCCAACCGCGACCCGGAG
GCCTTCGCCGACCCGGACGCCTTCGTCCCCGGCCGCGCCGACGGCGACCGGTCGCTCACC
CTGCTCGGCGGTTCCGCCGCCCCGGTCACCGAGGCGCTGGTCCGCCTCCAGACGGAAGCC
GCGGTGCGCGTCCTCGCCGAGCTGCGGCCCGGCCTGCGCACGGGCGCCGAGGTGCTGCAC
CGCATGCGCTCGGCGGTGCTGCGCGGCGTCCTGCGCTTCCCGGTCGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[292-352] 9.9e-10 PF00067
PF00067   p450
[41-415] 9.29999999999998e-45 G3DSA:1.10.630.10
G3DSA:1.10.630.10   Cyt_P450
[45-415] 1.70000295590053e-38 SSF48264
SSF48264   Cytochrome_P450
SignalP
[1-28] 0.199 Signal
Eukaryota   
TMHMM No significant hit
Cosmo_00100 Cosmo_00100   Cosmo_00120 Cosmo_00120
Page top