Cosmo_00110 Cosmo_00110   Cosmo_00130 Cosmo_00130

Cosmo_00120 : CDS information

close this sectionLocation

Organism
StrainDAUFPE 5622
Entry nameCosmomycin
Contig
Start / Stop / Direction10,725 / 12,005 / + [in whole cluster]
10,725 / 12,005 / + [in contig]
Location10725..12005 [in whole cluster]
10725..12005 [in contig]
TypeCDS
Length1,281 bp (426 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductdTDP-L-rhodosamine glycosyltransferase
Product (GenBank)CosG
Gene
Gene (GenBank)cosG
EC number
Keyword
Note
Note (GenBank)
  • glycosyltransferase
Reference
ACC
PmId
[16810496] Insights in the glycosylation steps during biosynthesis of the antitumor anthracycline cosmomycin: characterization of two glycosyltransferase genes. (Appl Microbiol Biotechnol. , 2006)
comment
cosmomycinsを含むanthracycline複合体retamycinの産生株Streptomyces olindensisの染色体14kb領域から、cosmomycin生合成に関連した13genesを同定。

cosG: Glycosyltransferase

aminodeoxysugarsを移動するglycosyltransferaseに似ているので、cosmomycin aglyconeの7- or/and 10-positionへL-rhodosamineを移動するglycosyltransferaseであると考えられた。

これを検証するために作製されたcosG不活化mutantは、cosmomycinとaglyconeは同じだが、position 7 and 10でL-rhodosamineの代わりにL-rhodinoseを持つ化合物のみを産生。
よってCosGは、L-rhodosamineの移動に関連するglycosyltransferaseである。
Related Reference
ACC
Q9L4U6
NITE
Acla_00210
PmId
[15911373] AknT is an activating protein for the glycosyltransferase AknS in L-aminodeoxysugar transfer to the aglycone of aclacinomycin A. (Chem Biol. , 2005)
[17685523] Characterization of rhodosaminyl transfer by the AknS/AknT glycosylation complex and its use in reconstituting the biosynthetic pathway of aclacinomycin A. (J Am Chem Soc. , 2007)
comment
BLAST id61%
Streptomyces galilaeus_aknS
Putative glycosyl transferase
[DoBISCUIT]dTDP-L-rhodosamine glycosyltransferase

--
[PMID: 15911373](2005)
AknKの実験に基づき代替基質として2-deoxyfucoseを用いたAknS/Tの実験。
AknS単独でも活性はあるが、AknTがないと活性は低い。

--
[PMID: 17685523](2007)
dTDP-L-rhodosamineがAknS/AknTの自然な基質であることを確認。
これまでに代替基質として使用されていたdTDP-2-deoxyfucoseより100倍、dTDP-daunosamineより1000倍速いKcat値を示す。

partner protein AknTは、L-rhodosamine移動のためのAknSの代謝回転速度を200倍に加速する。
AknTの存在はKm値に影響せず、kcatに影響する。AknS:AknTの最適比率は1:3。

aglycone, L-rhodosamine, 2-deoxyfucose, AknS/T, AknKでの産物は2糖型aclacinomycinが主要産物だったことから、3糖目は2-deoxyfucoseでなく、これまでの提唱通りL-rhodinoseでありそう。

close this sectionSequence

selected fasta
>dTDP-L-rhodosamine glycosyltransferase [CosG]
MRVLLTAFAQDAHLNGIVPLAWALRTAGHEVRVAGQPAAVESITRAGLTAVPVGDDHRMD
AVIPTVGPGMLMHHLDRDYLENRPERLSLDFLRASNAMLTATFYAQINNDSMIDALVDYA
RFWQPDLVLWEPFTFAGAVAAKATGAAHARLLAFPDLFANTHLTLRRRQAELPAELRDDP
LEEWLAYTLDRHGAAFDPDVVTGQWSVDQMPPGVRMELGLPTVPTRYVPYNGPGPAVVPD
WLRRPPERRRVCLTLGLTIRDTEFPNAVDVDDVFASLADLDVEVVATLSEKELAQVSRVP
DNTRVVDHVALLALLPSCSAIVHHGGAGTWATAAAYGVPQVALGWMWDAIYRAQRVEELG
AGLHLHSEGLTVEVLRDKLVRVLDDPSFTEGARRLRLAMLSAPSPNEVVPTLEKLTARHR
SVAAAR
selected fasta
>dTDP-L-rhodosamine glycosyltransferase [CosG]
GTGCGTGTACTGCTGACAGCCTTCGCACAGGACGCCCATCTCAACGGCATCGTTCCGCTC
GCCTGGGCGCTGCGGACCGCCGGGCACGAGGTCCGGGTGGCCGGCCAGCCGGCCGCGGTC
GAGAGCATCACCCGGGCCGGTCTGACGGCCGTGCCGGTGGGCGACGACCACCGGATGGAC
GCCGTCATCCCCACCGTCGGTCCCGGGATGCTCATGCACCACCTGGACCGCGACTACCTG
GAGAACCGCCCGGAGCGGCTGAGCCTCGACTTCCTGCGGGCGTCGAACGCGATGCTGACG
GCGACCTTCTACGCGCAGATCAACAACGACTCGATGATCGACGCGCTCGTCGACTACGCC
CGGTTCTGGCAGCCGGACCTGGTGCTCTGGGAGCCCTTCACCTTCGCCGGCGCCGTCGCC
GCCAAGGCGACCGGGGCCGCCCACGCCCGGCTCCTGGCCTTCCCCGACCTGTTCGCGAAC
ACCCACCTGACGCTGCGCCGGCGGCAGGCGGAACTGCCCGCGGAACTGCGCGACGACCCG
CTGGAGGAGTGGCTGGCGTACACCCTCGACCGGCACGGCGCCGCCTTCGACCCCGACGTG
GTCACGGGGCAGTGGTCGGTCGACCAGATGCCCCCCGGCGTGCGGATGGAGCTGGGGCTG
CCGACGGTGCCCACGCGTTACGTGCCGTACAACGGTCCGGGTCCGGCCGTCGTGCCGGAC
TGGCTGCGCCGGCCGCCGGAGCGCCGCCGGGTCTGCCTCACCCTCGGGCTCACCATCCGG
GACACGGAGTTCCCCAACGCCGTCGACGTCGACGACGTCTTCGCCTCGCTGGCCGACCTG
GACGTCGAGGTGGTGGCGACGCTGAGCGAGAAGGAACTCGCCCAGGTGTCGCGGGTGCCG
GACAACACGCGGGTGGTCGACCACGTGGCCCTGCTGGCCCTGCTGCCCAGTTGCTCGGCG
ATCGTCCACCACGGCGGTGCCGGGACGTGGGCGACCGCCGCCGCGTACGGGGTGCCGCAG
GTGGCGCTCGGCTGGATGTGGGACGCGATCTACCGCGCGCAGCGGGTGGAGGAGCTGGGG
GCGGGGCTGCACCTGCACTCCGAGGGCCTGACCGTCGAGGTCCTGCGCGACAAGCTCGTG
CGGGTGCTCGACGACCCCTCGTTCACGGAAGGCGCCCGACGGCTGCGTCTGGCCATGCTG
TCGGCGCCGAGCCCCAACGAAGTGGTGCCCACACTCGAGAAACTGACGGCGCGTCACCGC
TCGGTGGCCGCGGCCCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR004276 Glycosyl transferase, family 28 (Domain)
[3-149] 6.60000000000002e-12 PF03033
PF03033   Glyco_transf_28
IPR010610 Domain of unknown function DUF1205 (Domain)
[207-304] 1.4e-32 PF06722
PF06722   DUF1205
SignalP
[1-23] 0.211 Signal
Bacteria, Gram-positive   
[1-9] 0.327 Signal
Bacteria, Gram-negative   
[1-23] 0.322 Signal
Eukaryota   
TMHMM No significant hit
Cosmo_00110 Cosmo_00110   Cosmo_00130 Cosmo_00130
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