Cosmo_00120 Cosmo_00120   Cosmo_00140 Cosmo_00140

Cosmo_00130 : CDS information

close this sectionLocation

Organism
StrainDAUFPE 5622
Entry nameCosmomycin
Contig
Start / Stop / Direction12,028 / 13,011 / + [in whole cluster]
12,028 / 13,011 / + [in contig]
Location12028..13011 [in whole cluster]
12028..13011 [in contig]
TypeCDS
Length984 bp (327 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative dTDP-glucose 4,6-dehydratase
Product (GenBank)CosH
Gene
Gene (GenBank)cosH
EC number4.2.1.46
Keyword
  • L-rhodosamine
  • L-2-deoxyfucose
  • L-rhodinose
Note
Note (GenBank)
  • putative NDP-hexose 4,6 dehydratase
Reference
ACC
PmId
[16810496] Insights in the glycosylation steps during biosynthesis of the antitumor anthracycline cosmomycin: characterization of two glycosyltransferase genes. (Appl Microbiol Biotechnol. , 2006)
comment
cosmomycinsを含むanthracycline複合体retamycinの産生株Streptomyces olindensisの染色体14kb領域から、cosmomycin生合成に関連した13genesを同定。

cosH: NDP-hexose 4,6-dehydratase

cosHについては配列解析のみ。
NDP-deoxysugar生合成に関連。
Related Reference
ACC
Q56173
NITE
Grana_00110
PmId
[7476861] Identification of Streptomyces violaceoruber Tu22 genes involved in the biosynthesis of granaticin. (Mol Gen Genet. , 1995)
[9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998)
comment
Blast 7th, id69%, 1e-124
Streptomyces violaceoruber_graE(gra-orf17)
dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)

--
[PMID: 7476861](1995) abstract
ORF2はstrEに似ている。
E.coliでORF2を発現させると、dTDP-glucose→dTDP-4-keto-6-deoxy-D-glucoseへの変換を触媒することができた(controlの5倍)。
よって、ORF2はdTDP-glucose dehydrataseをコードする。

--
[PMID: 9831526](1998)
gra cluster genesのまとめ。renameあり。
ORF2→ORF17
ORF3→ORF16

close this sectionSequence

selected fasta
>putative dTDP-glucose 4,6-dehydratase [CosH]
MNILVTGAAGFIGSHFVRTLLSGGYPGHEDDRVTVLDKLTYAGTLNNLPAHHPRLTFVHG
DICDTTLLDKVFPGHEAVVHFAAESHVDRSVAGAEAFVRTNVLGTQALLEAALRHGIGVF
VQVSTDETYGSIAEGRWTEDEPLLPNSPYAASKASADLIARSYWRTHGLDVRITRCANNY
GPGQHPEKLVPLFVTRLLDGQPVPLYGDGSNLREWLHVDDHCHAVRLVLDRGRPGEVYNV
GGGTHLTNKEMTGRLLALCGRDWDLVRRVADRKGHDFRYAVDDSKIRRELGYAPRWSLED
GLRETVEWYAAHRDHWDAREEGADEGY
selected fasta
>putative dTDP-glucose 4,6-dehydratase [CosH]
GTGAACATCCTGGTGACCGGTGCGGCCGGCTTCATCGGCTCGCACTTCGTCAGAACGCTG
CTGAGCGGCGGGTACCCCGGGCACGAGGACGACCGGGTGACCGTCCTGGACAAGCTCACC
TACGCGGGGACGCTGAACAACCTGCCGGCGCACCACCCGCGGCTGACCTTCGTGCACGGC
GACATCTGCGACACCACCCTGCTGGACAAGGTGTTCCCGGGCCACGAGGCGGTCGTCCAC
TTCGCCGCGGAGTCGCACGTCGACCGCTCGGTGGCGGGCGCCGAGGCCTTCGTCCGCACC
AACGTGCTCGGCACGCAGGCCCTGCTCGAAGCGGCGCTGCGGCACGGGATCGGCGTCTTC
GTCCAGGTCTCCACGGACGAGACGTACGGTTCCATCGCCGAGGGGCGCTGGACGGAGGAC
GAGCCGCTGCTGCCCAACTCGCCCTACGCGGCGTCCAAGGCGTCGGCCGACCTCATCGCC
CGGTCGTACTGGCGCACCCACGGCCTCGACGTGCGCATCACCCGCTGCGCCAACAACTAC
GGCCCCGGCCAGCACCCCGAGAAGCTGGTCCCGCTGTTCGTCACCCGGCTCCTCGACGGG
CAGCCGGTCCCGCTGTACGGGGACGGCTCGAACCTGCGCGAGTGGCTGCACGTGGACGAC
CACTGCCACGCGGTGCGGCTGGTGCTGGACCGGGGCCGGCCGGGGGAGGTCTACAACGTC
GGCGGCGGCACCCACCTGACCAACAAGGAGATGACCGGCCGCCTCCTCGCACTCTGCGGA
CGCGACTGGGACCTCGTGCGGCGGGTGGCGGACCGCAAGGGGCACGACTTCCGGTACGCC
GTGGACGACTCCAAGATCCGCCGCGAACTGGGCTACGCGCCCCGCTGGTCGCTCGAGGAC
GGGCTGCGCGAGACGGTCGAGTGGTACGCCGCCCACCGTGACCACTGGGACGCCCGGGAG
GAAGGCGCCGACGAGGGGTACTAA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001509 NAD-dependent epimerase/dehydratase (Domain)
[3-241] 2.70000000000002e-68 PF01370
PF01370   Epimerase
IPR005888 dTDP-glucose 4,6-dehydratase (Family)
[2-317] 5.90000000000008e-122 TIGR01181
TIGR01181   DTDP_gluc_dehyt
IPR016040 NAD(P)-binding domain (Domain)
[1-177] 9.80000000000012e-66 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP No significant hit
TMHMM No significant hit
Cosmo_00120 Cosmo_00120   Cosmo_00140 Cosmo_00140
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