Ello_00270 Ello_00270   Ello_00290 Ello_00290

Ello_00280 : CDS information

close this sectionLocation

Organism
StrainTü2353
Entry nameElloramycin
Contig
Start / Stop / Direction27,601 / 28,482 / + [in whole cluster]
2,401 / 3,282 / + [in contig]
Location27601..28482 [in whole cluster]
2401..3282 [in contig]
TypeCDS
Length882 bp (293 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative glucose-1-phosphate thymidylyltransferase
putative dTDP-glucose synthase
Product (GenBank)NDP-D-glucose synthase
Gene
Gene (GenBank)rhaA
EC number2.7.7.24
Keyword
  • L-rhamnose
Note
Note (GenBank)
Reference
ACC
PmId
[18310024] Biosynthesis of elloramycin in Streptomyces olivaceus requires glycosylation by enzymes encoded outside the aglycon cluster. (Microbiology. , 2008)
comment
elloramycin clusterの同定論文。

elloramycinのsugar motiety L-rhamnose生合成に必要なrhaA-D genesは、PKSなどが含まれるclusterとは別のclusterに存在する。両subclustersがelloramycin生合成に必要であることは、rhaB不活化とS.lividansでの異種性plasmid発現により確認されている。

rhaA: NDP-D-glucose synthase

配列解析から、このORFはL-rhamnose生合成におけるNDP-D-glucose synthaseとして機能すると提唱されている。
Related Reference
ACC
P72017
PmId
[9084178] ( , )
[11302803] ( , )
comment
Blast 160th, id61%, 1e-100
Mycobacterium tuberculosis_rmlA(rfbA)
Glucose-1-phosphate thymidylyltransferase(EC 2.7.7.24)

--
[PMID: 9084178](1997)
rfbA gene cloning, E. coli発現タンパクで活性見ている。

--
[PMID: 11302803](2001)
dTTP + Glc-1P => dTDP-rhamnose 合成に関与する、RmlA, B, C, Dについて。
E. coli発現タンパク、RmlA, C, Dで活性みている。

close this sectionSequence

selected fasta
>putative glucose-1-phosphate thymidylyltransferase [NDP-D-glucose synthase]
MRGILLAGGTGSRLWPITRAVSKQLMPVFDKPMIYYPLSTLVMAGIKDILIITGPEERPQ
FERLLGDGSDFGLDIQFATQEHPNGIAEAFVIGEEFIGDEPVALILGDNIFHGVGLGRQL
ADYSKPEGGVVFAHAVSDPTAYGVVDFDENGVARSIEEKPKNPKSRYAVPGLYFYDSRVV
EIAKGLEPSARGELEITDVNKHYLERGELQVSVLDRGTVWLDTGTFQSLVQASEYVRVVE
ERQGMKIGCLEEAAWRAGFIDDEHLRALAQPLTKSGYGDYLLNLLAYEAEKKA
selected fasta
>putative glucose-1-phosphate thymidylyltransferase [NDP-D-glucose synthase]
ATGCGTGGAATTTTGCTTGCCGGTGGTACCGGCTCCCGACTGTGGCCGATCACCCGAGCC
GTGTCGAAGCAGCTGATGCCGGTCTTCGACAAGCCGATGATCTATTACCCCCTGTCCACC
CTCGTGATGGCGGGGATCAAGGACATTCTGATCATCACCGGACCGGAGGAGCGGCCCCAG
TTCGAGCGGCTTCTGGGAGATGGGTCCGATTTCGGACTCGACATCCAGTTCGCCACTCAG
GAGCACCCCAACGGAATTGCCGAGGCTTTCGTCATCGGCGAGGAATTCATCGGTGACGAG
CCCGTCGCCCTGATCCTCGGCGACAACATCTTCCACGGTGTCGGTCTCGGCCGACAGCTC
GCGGACTACAGCAAGCCCGAGGGTGGTGTGGTCTTCGCCCACGCCGTCTCCGACCCCACC
GCCTACGGTGTCGTCGACTTCGACGAGAACGGCGTCGCCCGCTCCATCGAGGAGAAGCCG
AAGAACCCCAAGTCCCGCTACGCGGTGCCGGGTCTCTACTTCTACGACAGCCGGGTCGTC
GAGATCGCCAAGGGCCTGGAGCCCAGCGCCCGCGGCGAGCTGGAGATCACCGACGTCAAC
AAGCACTACCTCGAGCGCGGCGAGCTCCAGGTCTCGGTCCTCGACCGCGGCACCGTCTGG
CTCGACACCGGCACCTTCCAGTCCCTCGTCCAGGCCTCCGAGTACGTCCGCGTGGTCGAG
GAGCGCCAGGGCATGAAGATCGGCTGCCTCGAAGAGGCCGCCTGGCGTGCCGGGTTCATC
GACGACGAGCACCTGCGCGCCCTCGCCCAGCCGCTGACCAAGAGCGGCTACGGCGACTAC
CTGCTCAACCTCCTTGCGTACGAGGCCGAGAAGAAGGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005835 Nucleotidyl transferase (Domain)
[3-235] 6.59999999999996e-63 PF00483
PF00483   NTP_transferase
IPR005907 Glucose-1-phosphate thymidylyltransferase, short form (Family)
[2-285] TIGR01207
TIGR01207   RmlA
SignalP
[1-20] 0.673 Signal
Eukaryota   
TMHMM No significant hit
Ello_00270 Ello_00270   Ello_00290 Ello_00290
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